초록

-Ⅳ. 연구개발결과
- 최적화된 심근세포 분화법 확립
- 최적화된 심근세포 분리정제법 확립
- 최적화된 심근세포체 형성 및 숙성법 확립을 위한 기반 기술 마련

(출처 : 요 약 문 3p)

Abstract ▼

Purpose:
Establishment of a standardized process and QA/QC of cardiomyocytes (CMC) differentiation from embryonic stem cells (ESC) and induced pluripotent stem cells(iPS) for evaluation of toxicity / drug efficay
- Development of a CMC differentiation method using embryonic body
- Isolation

Purpose:
Establishment of a standardized process and QA/QC of cardiomyocytes (CMC) differentiation from embryonic stem cells (ESC) and induced pluripotent stem cells(iPS) for evaluation of toxicity / drug efficay
- Development of a CMC differentiation method using embryonic body
- Isolation of differentiated CMC from mixed differentiated cells
- Maturation of CMC cell sheet or patch by electro-mechanical method
- Biological compatibility test of the cell sheet in vivo
- QA/QC for mass production of the ESC/iPS derived CMC

Contents
[1st year]
- Various cytokine treatment to embryonic body from ESC/iPS for efficient differentiation to CMC
- Targeting cytokines and transcription factors for CMC differentiation
- Establishment of a best method for CMC differentiation
[2nd year]
- Evaluation of CMC isolation methods including mitochondria tracker, CMC-specific metabolism, CMC surface marker
- Establishment of a best method for CMC isolation
[3rd year]
- Tissue engineering based CMC cell sheet/patch formation
- Maturation of CMC cell sheet using electrical stimulation
- Maturation of CMC cell sheet using mechanical stimulation
[4th year]
- In vivo transplantation of the matured cell sheet into normal or infarcted heart using nude mice
- Evaluation of the biologic compatibility of the cell sheet in vivo
[5th year]
- Establishment of process for mass production
- Development of criteria for QA/QC of each step
- Standardization of the process using QA/QC

Expected contribution
- Establishment of the best method for CMC differentiation
- Establishment of the best method for CMC isolation
- Establishment of the best method for CMC cell sheet and its maturation
- Standardizaion of process for CMC cell sheet production for toxicity or drug efficacy evaluation
- Establishment of biotechology leading to economic progress
- a step forward to achieve therapeutic application of stem cells to treat heart failure and ischemic heart disease

(출처 : SUMMARY 4p)

목차 Contents

• 표지 ... 1제 출 문 ... 2보고서 요약서 ... 2요 약 문 ... 3SUMMARY ... 4CONTENTS ... 4목차 ... 5제1장 연구개발과제의 개요 ... 6제2장 국내외 기술개발 현황 ... 7제3장 연구개발수행 내용 및 결과 ... 7 제 1절 1차년도 연구개발수행 내용 및 결과 ... 7 제 2절 2차년도 연구개발수행 내용 및 결과 ... 17 제 3절 3차년도 연구개발수행 내용 및 결과 ... 27제4장 목표달성도 및 관련분야에의 기여도 ... 34제5장 연구개발결과의 활용계획 ... 36제6장 연구개발과정에서 수집한 해외과학기술정보 ... 36제7장 연구시설·장비 현황 ... 36제8장 참고문헌 ... 36끝페이지 ... 48