보고서 정보
주관연구기관 |
중앙대학교 Chung Ang University |
연구책임자 |
김성기
|
참여연구자 |
박연주
,
이예은
|
보고서유형 | 최종보고서 |
발행국가 | 대한민국 |
언어 |
한국어
|
발행년월 | 2021-02 |
과제시작연도 |
2020 |
주관부처 |
농촌진흥청 Rural Development Administration(RDA) |
등록번호 |
TRKO202100009737 |
과제고유번호 |
1395062673 |
사업명 |
차세대바이오그린21(R&D) |
DB 구축일자 |
2021-09-18
|
키워드 |
브라시노스테로이드.식물호르몬.대사유전자 발현조절.함량조절 네트워크.다기능유용작물 개발.Brassinosteroids.Plant hormones.Regulation of metabolic genes.Regulatory network for endogenous hormones.Development of valuable plants.
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초록
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① bes1-k.o. x aba2-1 이중 결핍 돌연변이의 표현형과 ABA 함량 변화 분석을 통해 BES1에 의해 매개되는 종자 크기 결정 신호 전달 과정 확립
② Pst DC3000 감염상황에서 bzr1-1D의 pathogen resistance, 유전자 발현 및 호르몬 함량 변화 분석을 통해 BZR1에 의해 매개되는 SA 생합성 유전자 ICS1의 조절과 SA 신호 전달 인자의 조절 확인
③ BR target CK 대사 유전자 (IPT7) 선별을 완료하고, IPT7의 발현 패턴 및 BR 전사인자와의 상호결합 여부 분석
① bes1-k.o. x aba2-1 이중 결핍 돌연변이의 표현형과 ABA 함량 변화 분석을 통해 BES1에 의해 매개되는 종자 크기 결정 신호 전달 과정 확립
② Pst DC3000 감염상황에서 bzr1-1D의 pathogen resistance, 유전자 발현 및 호르몬 함량 변화 분석을 통해 BZR1에 의해 매개되는 SA 생합성 유전자 ICS1의 조절과 SA 신호 전달 인자의 조절 확인
③ BR target CK 대사 유전자 (IPT7) 선별을 완료하고, IPT7의 발현 패턴 및 BR 전사인자와의 상호결합 여부 분석 완료
④ 결핍 돌연변이 ipt7-1의 확보 및 genotyping 완료
⑤ B. distachyon 내 스테롤 및 BR 함량과 생합성 과정 조사 완료
⑥ 종자 특이적 BL synthase 발현 Brachypodium 형질전환체의 조직별 유전자 발현량, BR 및 1차 대사산물 함량, BL synthase 활성 확인을 통해 형질전환체의 종자에서 일어나는 종자 발달 메커니즘 확립
(출처 : 요약서 3p)
Abstract
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□ Purpose&Contents
The purpose of this study was to identify regulation networks for metabolism and activity of brassinosteroids (BRs), a plant hormone, and to develop a technology for practical use of multi-functional and useful crops. To achieve this goal, 1) Abscisic acid (ABA) biosynthesis an
□ Purpose&Contents
The purpose of this study was to identify regulation networks for metabolism and activity of brassinosteroids (BRs), a plant hormone, and to develop a technology for practical use of multi-functional and useful crops. To achieve this goal, 1) Abscisic acid (ABA) biosynthesis and signaling regulation mechanism by BR, 2) Salicylic acid (SA) biosynthetic gene expression regulation mechanism by BR, 3) Cytokinin (CK) content regulation gene expression regulation molecular mechanism by BR, 4) Development of technology for production of BR signal-based multi-functional useful crops using Brachypodium were investigated.
□ Results
Using bes1-k.o. x aba2-1 homozygous double deficient mutant, it was confirmed that BES1 regulates the gene expression of ABA2. Taken together with the results of previous studies, the expression of ABA2, one of the biosynthetic enzymes of ABA, is down-regulated by BES1, the transcription factor of BR, and the transcription of the negative regulator (ABI5) is reduced. This showed the result that the seed size was larger than that of the wild type by controlling the seed size determining genes (SHB1, MINI3, and IKU1/2). In addition, in bzr1-1D, the mechanism of regulation of SA content and SA signaling under Pst DC3000 infection was confirmed. As a result, under Pst DC3000 infection, for SA content regulation BZR1 directly binds to the promoter of the SA biosynthetic gene ICS1, and for SA signaling regulation BZR1 interacts with TGAs at the protein level and directly binds to the promoter of PR5 independently of NPR1. IPT7 was selected as a candidate gene for BR target CK metabolism. In the promoter of IPT7, the binding of BZR1 was stronger than that of BES1, indicating that BZR1 show greater activity in regulating IPT7 gene expression than BES1. A mutant ipt7-1 which lacks a candidate gene (IPT7) was obtained and confirmed with genotyping. In order to finally introduce the research results into the crops, the monocotyledonous model plant, Brachypodium distachyon, was analyzed for phytosterol composition and endogenous BR contents. In addition, BR biosynthetic enzymes in Brachypodium were identified and their functions were established. As a result, it was confirmed that brassinolide (BL), which is the most active BR, does not exist in Brachypodium, and based on this result, transgenic Brachypodium plants expressing BL synthase seed-specifically were produced. With analysis of transgenic Brachypodium, it was found that BL synthase expression on seed-specifically increased the qualitative and quantitative growth of the seed. By confirming the expression level of the seed development genes in transgenic Brachypodium, the mechanism of seed development caused by the newly synthesized BL in the seed of transgenic Brachypodium was established.
□ Expected Contribution
This study revealed that changes in several physiological phenomena can occur by a single molecule, BR, and changes in seed yield and stress resistance can also be caused. In addition, in order to final application of research outputs to crops, it was shown that Brachypodium can function as a platform plant for introduction to crop by developing monocotyledonous transgenic plants with enhanced BR activity seed-specifically. Taken together, the result of this study is expected to be a useful source technology that can be applied not only to agricultrue and horticulture, but to various fields such as increasing the production of biomass energy.
(출처 : SUMMARY 5p)
목차 Contents
- 표지 ... 1
- 제 출 문 ... 2
- 보고서 요약서 ... 3
- 국 문 요 약 문 ... 4
- Summary ... 5
- 목차 ... 6
- 제 1 장 연구 개발 과제의 개요 ... 7
- 제1절 연구 개발 목적 ... 7
- 제2절 연구 개발의 필요성 ... 7
- 제3절 연구 개발 범위 ... 8
- 제 2 장 연구 수행 내용 및 결과 ... 10
- 제 3 장 목표달성도 및 관련분야 기여도 ... 26
- 제1절 목표대비 달성도 ... 26
- 제2절 정량적 성과(논문게재, 특허출원, 기타)를 기술 ... 27
- 제 4 장 연구 결과의 활용 계획 ... 28
- 제 5 장 연구 개발 결과의 보안 등급 ... 28
- 제 6 장 연구시설·장비종합정보시스템에등록한 연구시설·장비현황 ... 28
- 제 7 장 연구개발과제의 대표적 연구실적 ... 29
- 제 8 장 기타사항 ... 29
- 제 9 장 참고문헌 ... 29
- 끝페이지 ... 30
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