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Kafe 바로가기주관연구기관 | 경희대학교 Kyung Hee University |
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연구책임자 | 박영두 |
참여연구자 | 김소정 , 김솔아 , 박지수 , 신윤희 , 이현민 , 전영지 |
보고서유형 | 최종보고서 |
발행국가 | 대한민국 |
언어 | 한국어 |
발행년월 | 2021-02 |
과제시작연도 | 2020 |
주관부처 | 농촌진흥청 Rural Development Administration(RDA) |
등록번호 | TRKO202100009773 |
과제고유번호 | 1395062941 |
사업명 | 차세대바이오그린21(R&D) |
DB 구축일자 | 2021-09-18 |
키워드 | 체세포 영양계변이.재분화개체.형질전환체.메틸롬.전이인자.Somaclonal variation.Regenerated plant.Transgenic plant.Methylome.Transposon. |
- 기 구축한 'CT001' pseudomolecule 보완 후 활용
- 재분화개체 집단의 유전체 변이 통합 분석 및 관련 유전자 기능 분석
- 형질전환체 집단의 유전체 변이 통합 분석 및 관련 유전자 기능 분석
- 재분화개체 및 형질전환체 집단 특이적 MITE 분석
- 선발된 표현형 이상 개체를 이용한 체세포영양계변이 분석
- 여교배를 통한 체세포영양계변이 완화 여부 확인
(출처 : 요약서 3p)
□ Purpose&Contents
○ Investigation of the gene functions and mechanism related to somaclonal variation through transgenic and regenerated Chinese cabbage plants
○ Development of recovery and detection methods for unintended genomic variations in transgenic and regenerated Chinese cabbage plant
□ Purpose&Contents
○ Investigation of the gene functions and mechanism related to somaclonal variation through transgenic and regenerated Chinese cabbage plants
○ Development of recovery and detection methods for unintended genomic variations in transgenic and regenerated Chinese cabbage plants
□ Results
○ DB construction and supplementation of pseudomolecules of inbred line 'CT001' used for tissue culture and transformation of Chinese cabbage
○ Integrative and in-depth analysis of somaclonal variations in regenerated group, progressed generation group and backcrossed group, and analysis of related gene functions
◎ Genome variation analysis
- Selection of 12 specifically generated exonic SNPs and analysis of related gene functions
◎ Epigenetic variation analysis
- Selection of 10 specifically generated DMRs and analysis of related gene functions
- Verification of changes in methylation pattern through methylation specific PCR using RCD1 primer set
○ Integrative and in-depth analysis of somaclonal variations in transgenic group, progressed generation group and backcrossed group, and analysis of related gene functions
◎ Genome variation analysis
- Selection of 485 specifically generated exonic SNPs and analysis of related gene functions
- Verification by HRM analysis using H1 primer
◎ Epigenetic variation analysis
- Selection of 11 specifically generated DMRs and analysis of related gene functions
- Verification of change in methylation pattern through methylation specific PCR using TCD8 primer
- Confirmation of gene expression level according to methylation pattern changes through qRT-PCR
○ Analysis of MITE activity specific to regenerated and transgenic Chinese cabbage plant groups
- Selection of 4 regenerated-specific active MITEs and 5 transgenic-specific active MITEs
- Analysis of the structure, characteristics, changes in activity, and transposition sites of the active MITEs
- Confirmation of MITE transposition activity through Southern hybridization and TAIL PCR
○ Variation analysis of somaclonal variants with late-flowering phenotypic abnormality
◎ Genome variation analysis
- Selection of 5,420 specifically generated SNPs and analysis of related gene functions
◎ Epigenetic variation analysis
- Confirmation of hyper-methylation in 6 genes related to flower development
□ Expected Contribution
○ Obtainment and utilization of genomic and epigenetic information of regenerated and transgenic Chinese cabbage populations
○ Understanding of unintented genomic variation according to somaclonal variation and investigation of mechanism
○ Development of molecular markers for early detection of somaclonal variation occurred during regeneration and transformation
○ Understanding of TE related to somaclonal variation and suggestion of potential use of selected MITEs as mutagens
○ Proposal of relieve and recovery methods for unintentional genomic variations caused by somaclonal variation
(출처 : SUMMARY 5p)
과제명(ProjectTitle) : | - |
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연구책임자(Manager) : | - |
과제기간(DetailSeriesProject) : | - |
총연구비 (DetailSeriesProject) : | - |
키워드(keyword) : | - |
과제수행기간(LeadAgency) : | - |
연구목표(Goal) : | - |
연구내용(Abstract) : | - |
기대효과(Effect) : | - |
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