초록

1차년: 야생형 대장균을 C9 지방산에 적응 배양하여, 이에 내성을 갖고 대사 속도가 향상된 균주 확립함.
2차년: 아젤라익산 생산 향상을 위한 유전자 규명 및 효율적인 오메가 산화경로 최적효소 선정함.
3차년: 합성생물학적 기법을 통한 최적 균주 구축 및 발효 최적화를 통한 아젤라익산 생산 증대를 야기함.

(출처 : 요약서 3p)

Abstract ▼

Purpose&Contents
The biochemical industry is a sustainable development technology as an industry that produces and utilizes useful chemical products by replacing existing chemical processes with biological processes. [1, 2]
This project aims to produce artificial microorganisms that can produc

Purpose&Contents
The biochemical industry is a sustainable development technology as an industry that produces and utilizes useful chemical products by replacing existing chemical processes with biological processes. [1, 2]
This project aims to produce artificial microorganisms that can produce medium-chain dicarboxylic acids, a high value-added substance used as a raw material for cosmetics.

For the production of medium-chain dicarboxylic acids, an ω-oxidation pathway was established in Escherichia coli. E. coli was adaptively cultured in nonanoic acid for 30 days to enhance toxicity and intracellular transport to nonanoic acid. The adapted mutant E. coli was selected and the production of azelaic acid was improved. The production can be improved through the establishment of an efficient ω-oxidation pathway by securing new enzymes, improvement of enzymes using protein engineering methods, improvement of substrate transport and production material emission.

Results
1) Establishment of azelaic acid production strain
· Isolation and development of strains with improved metabolic rate and resistance to nonanoic acid by adaptive cultivation
· Confirmation of azelaic acid production from nonanoic acid by introducing the ω-oxidation pathway into the production strain
2) Establishment of ω-oxidation pathway for azelaic acid production
· Acquisition of monooxygenase from various microorganisms (M. aquaeolei, B. megaterium, S. cerevisiae, C. tropicalis, P . putida)
· 1g/L of nonanoic acid is converted to 221 mg/L of azelaic acid, which is 22% conversion rate.

Expected Contribution
1. Through this study, research in metabolic engineering and synthetic biology can be promoted by identifying the activity and substrate specificity of various genes. Promote research in the future biological industry using synthetic biology, such as the use of various genes and the development of artificial microorganisms.
2. The manufacturing technology of a new biological system will not only become the driving force to drive the development of the original technology for the development of parts and materials and system design necessary for the production and synthesis of artificial genomes, the future science, Part material and design technology can be secured.
3. A comprehensive gene control system can be used in the biochemical industry, which aims at eco-friendly mass production of biofuels and biochemical products.

(출처 : Summary 5p)

목차 Contents

• 표지 ... 1
• 제출문 ... 2
• 보고서 요약서 ... 3
• 국문 요약문 ... 4
• Summary ... 5
• 목차 ... 6
• 제 1 장 연구 개발 과제의 개요 ... 7
• 제 1 절 연구 개발 목적 ... 7
• 제 2 절 연구 개발의 필요성 ... 8
• 제 3 절 연구 개발 범위 ... 9
• 제 2 장 연구 수행 내용 및 결과 ... 11
• 제 3 장 목표달성도 및 관련분야 기여도 ... 23
• 제 1 절 목표대비 달성도 ... 23
• 제 2 절 정량적 성과(논문게재, 특허출원, 기타)를 기술 ... 24
• 제 4 장 연구 결과의 활용 계획 ... 25
• 제 5 장 연구 개발 결과의 보안 등급 ... 26
• 제 6 장 연구시설·장비종합정보시스템에등록한 연구시설·장비현황 ... 27
• 제 7 장 연구개발과제의 대표적 연구실적 ... 28
• 제 8 장 기타사항 ... 29
• 제 9 장 참고문헌 ... 31
• 끝페이지 ... 33