[보고서]화분매개충에 대한 농자재 독성 및 위해성평가 개선연구

전경미

화분매개충에 대한 농자재 독성 및 위해성평가 개선연구
A study on the refinement of risk assessment for pollinating insects 원문보기

보고서 정보
주관연구기관	국립농업과학원 National Institute of Agricultural Sciences
연구책임자	전경미
참여연구자	김보선 , 김아름누리 , 김주영 , 박경훈 , 박연기 , 박홍현 , 백민경 , 유아선 , 윤형주 , 이환 , 문지홍 , 오진아 , 최용수
보고서유형	최종보고서
발행국가	대한민국
언어	한국어
발행년월	2021-02
과제시작연도	2020
주관부처	농촌진흥청 Rural Development Administration(RDA)
과제관리전문기관	농촌진흥청 Rural Development Administration
등록번호	TRKO202100009944
과제고유번호	1395066057
사업명	농업과학기반기술연구(R&D)
DB 구축일자	2021-10-02
키워드	서양뒤영벌.꿀벌 반야외시험.위해성평가.농약.Bumblebees.Honeybee semi-field test.Risk assessment.Pesticides.

초록 ▼

○ 서양뒤영벌 급성 접촉 및 섭식 독성시험법 확립
○ 네오니코티노이드계 농약 4종에 대한 서양뒤영벌 독성평가
○ 토마토 주요사용 농약에 대한 서양뒤영벌 엽상잔류독성(RT₂₅) 영농정보 제공(영농기술·정보기관 제출)
○ 꿀벌 반야외시험법을 활용한 농약 3종 독성평가
- 농약 3종 독성평가(이미다클로프리드 미탁제, 클로르피리포스 수화제, 사이퍼메트린 유제)
○ 꿀벌 반야외시험법 주요 Index 데이터 구축 및 평가기준 제시
- 꿀벌치사개체, 비행활동, 봉군상태, Brood termin

Abstract ▼

□ Purpose&Contents
○ Establishment of acute toxicity tests for bumblebees and assessement of toxcity of pesticides on bumblebees
○ Providing information RT₂₅ of bumblebees for pesticides used on tomato crops
○ Accumulation of main index and providing the evaluation criteria of honeybee semi-field tests

□ Results
○ Establishment of OECD Guidelines of acute contact and oral toxicity tests for bumblebees (Bombus terrestris)
○ Assessment of contact and oral toxicity of four neonicotinoid insecticides to bumblebees (Bombus terrestris)
○ Determination of RT₂₅ of bumblebees for pesticides used on tomato crops
○ A study on the effects of 3 pesticides on honeybee brood under semi-field conditions
- 3 Pesticides (imidacloprid ME, Chlorpyrifos WP, Cypermethrin EC)
- The mortality of the honey bees, flight activity, condition of the colonies, brood termination rate (BTR), brood index (BI),compensation index (CI)
○ Publication of the method guideline of honeybee(Apis Mellifera L.) brood test under semi-field conditions in the agricultural chemical regulation laws of Korea (2019.Mar.)

□ Expected Contribution
○ Technical support for the safe use of pesticides with bumblebees
○ Improvement of pesticide risk assessment scheme for bees in Korea
○ Preemptive response to global pollinator issues related with pesticides
○ Professional manpower training in the field of bee toxicity tests

(출처 : SUMMARY 6p)

목차 Contents

표지 ... 1
제출문 ... 2
보고서 요약서 ... 3
국 문 요 약 문 ... 5
Summary ... 6
목차 ... 7
제 1 장 연구 개발 과제의 개요 ... 8
제 1 절 연구 개발 목적 ... 8
제 2 절 연구 개발의 필요성 ... 8
제 3 절 연구 개발 범위 ... 10
제 2 장 연구 수행 내용 및 결과 ... 11
제 1 절 서양뒤영벌 급성독성시험법 확립 및 농약 독성평가(전경미, ’18-’20) ... 11
제 2 절꿀벌반야외시험법국내데이터구축및평가기준제시연구(전경미, ’18-’20) ... 37
제 3 장 목표달성도 및 관련분야 기여도 ... 84
제 1 절 목표대비 달성도 ... 84
제 2 절 정량적 성과(논문게재, 특허출원, 기타)를 기술 ... 85
제 4 장 연구 결과의 활용 계획 ... 86
제 5 장 연구 개발 결과의 보안 등급 ... 87
제 6 장 연구시설·장비종합정보시스템에 등록한 연구시설·장비 현황 ... 88
제 7 장 연구개발과제의 대표적 연구실적 ... 89
제 8 장 기타사항 ... 90
제 9 장 참고문헌 ... 91
끝페이지 ... 93

표/그림 (92)

표 Survey on the major pesticides used for tomatoes
표 Photographs of trial materials for acute toxicity tests: A, feeding devices ((left) a syringe with a tip), (right) a syringe without a tip); B, a single housing cage; C, a trial setup (the rack is inclined to the syringes for ad libitum feeding)
표 Photographs of trial materials and procedure for acute contact toxicity test: A, multipette M4 pipette; B, bumblebees placed on W-folded papers; C, individual treatment on the dorsal side of the thorax of bumblebees
표 Procedure of acute toxicity tests for bumblebees
표 Sublethal effects and mortality assessments, adapted from OECD (2017a, 2017b)
표 The view of the tomato greenhouse
표 The change of feeding method for the foliage residual toxicty test on bumblebees
표 Final selection of cage and feeding method for the foliage residual toxicty test on bumblebees
표 Pesticide spraying and leaf collection
표 The process of the foliage residual toxicty test on bumblebees
표 The comparison of honeybee and bumblebee toxicity of residues on foliage
표 The selection process of pesticides for the test
표 Top 10 insectides used by tomato growers
표 Experimental temperature and humidity conditions in acute toxicity tests
표 Mean weight of bumblebees in acute contact toxicity test
표 Number of affected bumblebees in acute contact toxicity tests
표 Mean weight of bumblebees in acute oral toxicity tests
표 Number of affected bumble bees in acute oral toxicity tests
표 Mean weight of bumblebees in acute contact toxicity tests
표 Total number of affected bumblebees in acute contact toxicity tests
표 Total number of affected bumblebees in acute oral toxicity tests
표 The acute toxicity (LD50) values of four neonicotinoids to bumblebees
표 Determination of RT25 of bumblebees for pesticides used on tomato crops
표 Total mean weight, temperature and relative humidity during the tests
표 Number of dead bumblebees by sampling time after application of pesticides to the crops
표 Determination of RT25 of bumblebees for pesticides used on tomato crops
표 Total mean weight, temperature and relative humidity during the tests
표 Number of dead bumblebees by sampling time after application of pesticides to the crops
표 Total mean weight of bumblebees, temperature and relative humidity during the tests
표 Mortalities of bumblebees exposed to tomato leaves collected at 4 h and 24 h after application of pesticides to the crops
표 Time scale of the test and assessments made
표 The test schedule for honeybee semi-field test(2018)
표 Evaluation of mortality of honey bees
표 Evaluation of flight activity
표 Expected brood stage at each assessment day for eggs at BFD 0
표 Photobox
표 Schematic representation of the important steps of the larval acute and chronic toxicity test (D=day; RH=relative humidity). Adapted from OECD (2016)
표 Volume and composition of larvae diet
표 Amount of imidacloprid in diet for chronic toxicity test
표 The test schedule for honeybee semi-field test(2019)
표 Honeybee larval repeated exposure toxicity test
표 Schematic representaion of the important steps of the larval repeated exposure test
표 Observations
표 The collection of bees(2 day old)
표 Honeybee chronic oral toxicity test(10 day)
표 Cultivation of Brassica napus(2020)
표 The test schedule for honeybee semi-field test(2020)
표 Flight activity of honey bees during the exposure phase in the treatments
표 Number of bees per colony in the control group
표 Number of bees per colony in diflubenzuron WP 25% group
표 Number of bees per colony in imidacloprid ME 20% group
표 Brood termination rates of the marked cells in the case of eggs at BFD 0
표 Mean brood index and compensation index
표 BTR with different number of observed eggs
표 Toxicological endpoints of imidacloprid at 48 and 72 h after single exposure
표 Toxicological endpoints of imidacloprid at D8, D15, and D22 after repeated exposure
표 Evolution of mortality after oral application of five concentration of imidacloprid from day 4(D4) to day 22(D22)
표 Laval mortality, pupal mortality and adult emergence rate of honeybees exposed to imidacloprid. The comparisons between exposed groups and control were performed with Chi-square test and Fisher′s exact test (*P≤0.05, **P≤0.01 and ***P≤0.001) (n=36 individuals per group from 3 replicates)
표 Flight activity of honey bees during the exposure phase in the treatments
표 Number of bees per colony in the control group
표 Number of bees per colony in diflubenzuron group
표 Number of bees per colony in chlorpyrifos group
표 Brood termination rates of the marked cells in the case of eggs at BFD 0
표 BTR with different number of observed eggs
표 Brood index and compensation index based on 200 eggs
표 Residual amount of pesticide residue detected in rape flowers
표 Compositions of 30 BLAST-matched sequences of 30 DNA sequences from the unexposed bees (a), and 30 BLAST-matched sequences of 30 DNA sequences from the exposed bees (b) at the order level
표 Average copy numbers of 16SrRNA gene per gut out of the unexposed and exposed honeybees to the pesticide of carbaryl
표 Mean temperature and relative humidity
표 Cumulative mortality and adult emergence rate of honey bees
표 Mean deformation rate of honey bees
표 Deformation rate by symptoms
표 Observed symptoms of newly emerged adult bees. (A) Surviving normal (SN); (B) Deformed with short wings (DSW); (C) Deformed with tangled wings (DTW); (D) Abnormal abdomen shape (AAS); (E) Deformed antennae (DA). Scale bar = 1 mm
표 Mean weight and length of honey bees
표 Mortality and food consumption of the honey bee exposed to dimethoate for 10 days
표 Percentage of symptoms observed in dead bees
표 Observed symptoms of the honey bee exposed to dimethoate Extended proboscis (EP) : 주둥이(proboscis)를 길게 내밀고 죽은 경우, Twisted bodies (TB) : 머리와 몸통이 비틀어진 채로 죽은 경우, Sting in release out position and anus with excreta (SE) : 배설물과 벌침이 밖으로 나와 있는 경우,Normal (N) : 주둥이를 길게 내밀지 않고, 몸이 뒤틀리지 않고, 배설한 흔적이 없는 정상적인 경우
표 Data on the climatic conditions during the experimental period
표 Brood termination rates of the marked cells in the case of eggs at BFD 0
표 Brood termination rates by the number of eggs
표 BTR with different number of observed eggs
표 Residual amount of pesticide residue detected in rape flowers
표 Climatic conditions during honeybee semi-field study in 2016
표 Climatic conditions during honeybee semi-field study in 2017
표 Climatic conditions during honeybee semi-field study in 2018
표 Climatic conditions during honeybee semi-field study in 2019
표 Climatic conditions during honeybee semi-field study in 2020
표 Mortality of worker bees during the experimental phase
표 Flight activity of honey bees during the exposure phase in the treatments
표 Brood termination rates of the marked cells in the case of eggs at BFD 0
표 Brood index
표 Brood compensation index

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연합인증

화분매개충에 대한 농자재 독성 및 위해성평가 개선연구
A study on the refinement of risk assessment for pollinating insects 원문보기