초록

○ 인삼, 차나무 유전물질 안전보존을 위한 DNA stock 시스템 구축
○ DNA profiling을 위한 분자마커 선발 및 분석조건을 확립하여 DNA profiling DB 구축
○ DNA profiling DB를 활용한 인삼, 차나무 유전자원 다양성 분석 수행으로 자원관리 기반 마련

(출처 : 요약서 3p)

Abstract ▼

□ Purpose&Contents
Importance of plant genetic resources (PGR) is increasing as they are breeding materials for developing new varieties and sources of bio-engineering research and product. Establishment of PGR management at molecualr level is needed for defense of benefit sharing and intellectua

□ Purpose&Contents
Importance of plant genetic resources (PGR) is increasing as they are breeding materials for developing new varieties and sources of bio-engineering research and product. Establishment of PGR management at molecualr level is needed for defense of benefit sharing and intellectual property along with Nagoya protocol.
The objectives of this project are to establish conservation system of genetic material and construct DNA profiling database of ginseng and tea germplasm for efficient PGR managemnet.

□ Results
A total of 1,109 national ginseng germplasm conserved in three sub-genebank were collected and DNA stock was constructed with their extracted DNA. Conservation of DNA used two systems: 1) deep-freezer in liquid state and 2) descicator in dried state. A total of 21 SSR markers were selected for the genotyping and the genetic characterization of ginseng germplasm. DNA profiling database was made from allele genotyping of each accession. The profiling DB was used for genetic divesity analysis of whole ginseng germplasm in whichthe genetic differentiation and gene flow were 9.4% (PhiPT = 0.094) and 4.822, respectively. There are no significant differences among collected area and landrace showed also low genetic differentiation (0.034) and high gene flow (14.322), indicating low genetic diversity of ginseng germplasm. A total of 464 national tea germplasm also collected and DNA stock was constructed with the same method used in ginseng germplasm. A total of 21 SSR markers were selected and used for genotyping of tea germplasm. After analysis of genetic diversity, tea germplasm were divided into four groups of which the genetic differentiation was only 1.4%. Phytochemicals such as catechin and caffeine were analyzed for two years and core collection was developed from an incorporation of phytochemicals with genetic analysis result.

□ Expected Contribution
DNA stock and profiling DB are useful for germplasm management and conservation such as evaluation of germplasm redundancy, diversity analysis, and germplasm collection.
Developed core collection of tea germplasm will be used in breeding program and functional material research.

(출처 : SUMMARY 5p)

목차 Contents

• 표지 ... 1
• 제 출 문 ... 2
• 보고서 요약서 ... 3
• 국 문 요 약 문 ... 4
• Summary ... 5
• 목차 ... 6
• 제 1 장 연구 개발 과제의 개요 ... 7
• 제1절 연구 개발 목적 ... 7
• 제2절 연구 개발의 필요성 ... 7
• 제3절 연구 개발 범위 ... 8
• 제 2 장 연구 수행 내용 및 결과 ... 9
• 제1절 인삼 농업유전자원의 효율적 관리를 위한 분자유전학적 연구 ... 9
• 제2절 차나무 농업유전자원의 효율적 관리를 위한 분자유전학적 연구 ... 18
• 제 3 장 목표달성도 및 관련분야 기여도 ... 32
• 제1절 목표대비 달성도 ... 32
• 제2절 정량적 성과(논문게재, 특허출원, 기타)를 기술 ... 32
• 제 4 장 연구 결과의 활용 계획 ... 34
• 제1절 추가연구의 필요성 ... 34
• 제2절 연구개발 결과의 활용방안 ... 34
• 제3절 기대성과 및 파급효과 ... 34
• 제 5 장 연구 개발 결과의 보안 등급 ... 35
• 제 6 장 연구시설·장비종합정보시스템에 등록한 연구시설·장비 현황 ... 36
• 제 7 장 연구개발과제의 대표적 연구실적 ... 37
• 제 8 장 기타사항 ... 38
• 제 9 장 참고문헌 ... 39
• 끝페이지 ... 40