초록

본 연구는 당대사를 개선할 수 있는 형질전환 벼를 육성하는 연구로서, 형질전환 벼의 고정계통을 선발하였고, 거미 실크피브로인 유전자가 안정적으로 도입되고 발현하여 목적단백질의 생산이 잘 이루어짐. 그리고 형질전환 벼를 식이한 동물실험에서 혈당과 간기능과 관련된 지표가 개선되어 당대사 개선 효과가 인정되었고, 특히 지방세포의 수와 크기의 감소가 뚜렷한 변화로 관찰됨.

(출처 : 요약서 3p)

Abstract ▼

Purpose&Contents
○ Breeding of transgenic rice event for improving glucose metabolism as a medicinal material
- Selection and genetic analysis of transgenic rice(T7 plants)
- Analysis of major components and agricultural characteristics of transgenic rice(T8 plants)
- Molecular character

Purpose&Contents
○ Breeding of transgenic rice event for improving glucose metabolism as a medicinal material
- Selection and genetic analysis of transgenic rice(T7 plants)
- Analysis of major components and agricultural characteristics of transgenic rice(T8 plants)
- Molecular characterization of transgenic rice(T9 plants)

○ Presenting verification data on the treatment effect of type 2 diabetes of transgenic rice for improving glucose metabolism
- Verification of treatment improvement effect and investigation of related molecular mechanisms in naturally occurring diabetes model mice
- Verification of the effect of reducing insulin resistance in high-fat diet diabetes-induced mice and investigation of related molecular mechanisms
- Insulin resistance mechanism of fat and skeletal muscle tissues in high fat diet diabetic mice model
- Investigation of mechanism of signal transduction of fibroin protein in liver and adipose tissue

Results
○ Selection of OsAvMaSP-113 multiple generations(T7, T8, T9 plants) of transgenic rice for improving glucose metabolism, and confirmattion of introduced GluC promoter, 35S promoter, AvMaSp gene, bar gene and nos terminator were used by PCR
○ Target protein content of OsAvMaSp Line 113(T8, T9, T10 seeds), the transgenic rice for improving glucose metabolism, the total protein content was not different from that of the control, Dongjin rice, and in the case of the spider silk protein content, the transgenic rice Only 451ug, 436ug, 446ug/100g were high
○ Backbone DNA (pZP200 vector) was inserted into the host and whether it was not inserted using an antibiotic resistance marker, it was confirmed that it was not inserted into the transgenic rice
○ Analysis of anti-nutritional components such as phytic acid and trypsin inhibitors and general component analysis of transgenic rice, there was no difference from Dongjin rice, the control.
○ Corresponding results of diabetic mice fed transgenic rice are as following: 1) blood glucose levels were reduced after exposure to the transgenic rice; fatty liver appeared to be changed to normal hepatic tissues, 2) Stamp2 or phosphorylated AMPk proteins contents in hepatic tissues were increased, 3) In adipose tissues, IRS1 phosphorylation was increased and AMPk phosphorylation and Stamp2 protein contents were decreased, 4) In skeletal mucscles, AMPk phosphorylation and Stamp2 proteins were reduced after exposed to the transgenic rice, 5) Fibroin protein seems to act hepatocytes directly and thus induced AMPk phosphorylation, 6) Finally, it could be suggested that the biological acition of transgenic rice tested in this study is associated with mechanisms relate to energy metabolism and insulin sensitivity.

Expected Contribution
○ Industrialization through technology transfer/technology implementation, etc., using patent rights obtained based on the experimental results using transgenic rice that expresses the spider fibroin protein developed in this study
○ The research results obtained from conducting this study are small-scale studies and are used as the basis for further studies in the preclinical/clinical stage and the safety test study of transgenic rice
○ Both the dietary form and the form of recombinant protein of transgenic rice have high potential for medical application and industrialization
○ Expected to reduce social costs by KRW 73.5 billion by preventing diabetes with the diet of transgenic rice introduced with the silk fibroin gene
○ Competitive in the international seed market by developing transgenic rice varieties for improving glucose metabolism

(출처 : Summary 6p)

목차 Contents

• 표지 ... 1
• 제 출 문 ... 2
• 보고서 요약서 ... 3
• 국 문 요 약 문 ... 4
• Summary ... 6
• 목차 ... 8
• 제 1 장 연구 개발 과제의 개요 ... 9
• 제1절 연구 개발 목적 ... 9
• 제2절 연구 개발의 필요성 ... 9
• 제3절 연구 개발 범위 ... 11
• 제 2 장 연구 수행 내용 및 결과 ... 12
• 제1절 이론적 접근방법 ... 12
• 제2절 실험적 접근방법 ... 13
• 제3절 연구내용 ... 15
• 제4절 연구결과 ... 22
• 제 3 장 목표달성도 및 관련분야 기여도 ... 60
• 제1절 목표대비 달성도 ... 60
• 제2절 정량적 성과(논문게재, 특허출원, 기타)를 기술 ... 63
• 제 4 장 연구 결과의 활용 계획 ... 64
• 제1절 추가연구의 필요성 ... 64
• 제2절 타 연구에의 응용 ... 64
• 제3절 기업화 추진방안 ... 64
• 제4절 현재 추진중인 추가적인 논문게재, 산업재산권 출원 사항 ... 64
• 제 5 장 연구 개발 결과의 보안 등급 ... 65
• 제 6 장 연구시설·장비종합정보시스템에 등록한 연구시설·장비 현황 ... 66
• 제 7 장 연구개발과제의 대표적 연구실적 ... 67
• 제 8 장 기타사항 ... 68
• 제1절 중요 연구변경사항 ... 68
• 제 9 장 참고문헌 ... 69
• 끝페이지 ... 71