![CX3CR1 ablation results in delayed viral clearance from peripheral lymphoid tissues. (A) Viral burden in peripheral lymphoid during JE progression. (B) Infectious JEV in sera and viral burden in CNS tissues during JE progression. Viral burden in popliteal LNs, spleen, and brain of CX3CR1+/+ and CX3CR1-/- mice was assessed by real-time qRT-PCR at the indicated days after infection with JEV via footpad inoculation. Viral RNA load was expressed by viral RNA copy number per microgram of total RNA. The levels of infectious JEV in sera were determined by focus-forming assay. (C) Confocal imaging for detection of JEV Ag+CX3CR1+CD11c+ DCs in popliteal LNs. Sections of popliteal LNs derived from CX3CR1+/gfp and CX3CR1gfp/gfp mice infected with JEV via footpad inoculation were co-stained for JEV Ags (E and NS1 [PE red)] and DC marker CD11c (APC purple) at 2 dpi. The CX3CR1gfpCD11c+ DCs co-localizing with JEV Ags in lower magnification images (upper pictures) and higher magnification images (lower pictures) are denoted by white arrows. GC is the abbreviation for germinal center. (D) Visualization of JEV in the CNS. Brain sections obtained from JEV-infected CX3CR1+/gfp and CX3CR1gfp/gfp mice were co-stained with JEV Ags (E and NS1 [PE red]) and nuclear stain DAPI (blue) at 5 dpi. Images are representative of sections derived from at least five mice per group. Data show the average ± SEM of levels derived from at least three independent experiments (n=4-5). **p<0.01; ***p<0.001 for CX3CR1+/+ versus CX3CR1-/- mice at the indicated dpi](https://nrms.kisti.re.kr/bitextimages/TRKO202100016735/TRKO202100016735_14_image_2.png "CX3CR1 ablation results in delayed viral clearance from peripheral lymphoid tissues. (A) Viral burden in peripheral lymphoid during JE progression. (B) Infectious JEV in sera and viral burden in CNS tissues during JE progression. Viral burden in popliteal LNs, spleen, and brain of CX3CR1+/+ and CX3CR1-/- mice was assessed by real-time qRT-PCR at the indicated days after infection with JEV via footpad inoculation. Viral RNA load was expressed by viral RNA copy number per microgram of total RNA. The levels of infectious JEV in sera were determined by focus-forming assay. (C) Confocal imaging for detection of JEV Ag+CX3CR1+CD11c+ DCs in popliteal LNs. Sections of popliteal LNs derived from CX3CR1+/gfp and CX3CR1gfp/gfp mice infected with JEV via footpad inoculation were co-stained for JEV Ags (E and NS1 [PE red)] and DC marker CD11c (APC purple) at 2 dpi. The CX3CR1gfpCD11c+ DCs co-localizing with JEV Ags in lower magnification images (upper pictures) and higher magnification images (lower pictures) are denoted by white arrows. GC is the abbreviation for germinal center. (D) Visualization of JEV in the CNS. Brain sections obtained from JEV-infected CX3CR1+/gfp and CX3CR1gfp/gfp mice were co-stained with JEV Ags (E and NS1 [PE red]) and nuclear stain DAPI (blue) at 5 dpi. Images are representative of sections derived from at least five mice per group. Data show the average ± SEM of levels derived from at least three independent experiments (n=4-5). **p<0.01; ***p<0.001 for CX3CR1+/+ versus CX3CR1-/- mice at the indicated dpi")
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| 주관연구기관 | 전북대학교 Chonbuk National University |
|---|---|
| 연구책임자 | 어성국 |
| 보고서유형 | 최종보고서 |
| 발행국가 | 대한민국 |
| 언어 | 한국어 |
| 발행년월 | 2021-03 |
| 과제시작연도 | 2020 |
| 주관부처 | 과학기술정보통신부 Ministry of Science and ICT |
| 연구관리전문기관 | 한국연구재단 National Research Foundation of Korea |
| 등록번호 | TRKO202100016735 |
| 과제고유번호 | 1711113367 |
| 사업명 | 개인기초연구(과기정통부)(R&D) |
| DB 구축일자 | 2022-01-29 |
| 키워드 | 대식세포 분극화.플라비바이러스.macrophage polarization.flavivirus.ROS.cAMP.microRNA. |
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