초록

Abstract ▼

□ 연구개요
The six major Brassica species in the Triangle of U comprise three economically important diploid species B. rapa (AA), B. nigra (BB), and B. oleracea (CC). Raphanus sativus (RR), although not included in the Triangle of U, is also an important crop all over the world, particularly in Kore

□ 연구개요
The six major Brassica species in the Triangle of U comprise three economically important diploid species B. rapa (AA), B. nigra (BB), and B. oleracea (CC). Raphanus sativus (RR), although not included in the Triangle of U, is also an important crop all over the world, particularly in Korea. In fact, several breeding efforts have been conducted to generate stable intergeneric synthetic hybrids between R. sativus and other Brassica species to maximize utility of plant parts and study the effects of intergeneric hybridization. To facilitate interspecies and intergeneric breeding programs, it is important to identify the genomic origin of chromosomes in resulting hybrids or introgression lines. In this project, I wanted to develop chromosome-specific oligonucleotide probes to distinguish individual chromosomes and subgenomes in hybrids between B. rapa and R. sativus.

□ 연구 목표대비 연구결과
The main objectives of this project are to generate high-quality oligonucleotide probes unique to each chromosomes of B. rapa and R. sativus using bioinformatics tools and to validate the chromosome-specificity of these chromosome-specific probes using fluorescence in situ hybridization.
My data show that I fully achieved all objectives presented in this project. I successfully developed chromosome-specific probes that can distinguish the ten and nine chromosomes of B. rapa and R. sativus, respectively. However, experiments to generate data on the practical usefulness of these probes using actual hybrids have not been performed because of the early abortion of the project.

□ 연구발성과의 활용 계획 및 기대효과(연구개발결과의 중요성)
The chromosome-specific oligonucleotide probes developed in this project can be utilized to analyze the subgenomes between new synthetic hybrid lines between the AA and RR allotetraploid genomes, either in the F1 or backcross lines to trace chromosomal introgression. This probes can also be used to analyze chromosomal rearrangements between related synthetic and natural allotetraploids.
Because I have shown the practicality of this method using the AA and RR genomes, this methodology can be expanded to other Brassica genomes such as AA and CC to create a greater arsenal of probes that can be deployed for analyzing species in the U’s Triangle and that of R. sativus. These probes will be a very useful tool to supplement the active breeding research involving these species.

(source : 연구결과 요약문 2p)

목차 Contents

• COVER ... 1
• 연구결과 요약문 ... 2
• 목차 ... 3
• 1. 연구개발과제의 개요 ... 4
• 1.1. Background ... 4
• 1.2. Research objectives ... 4
• 1.3. Necessity of the research ... 4
• 1.4. Status of the technology ... 5
• 1.5. Application and expected effects ... 5
• 1.6. Preliminary research results ... 5
• 2. 연구개발과제의 수행 과정 및 수행 내용 ... 6
• 2.1. Oligoprobe design ... 7
• 2.2. Oligoprobel abeling ... 7
• 2.3. FISH analysis ... 8
• 2.4. Project timeline and yearly research goals and budget ... 8
• 3. 연구개발과제의 수행 결과 및 목표 달성 수준 ... 9
• 1) 정성적 연구개발성과(연구개발결과) ... 9
• 2) 세부 정량적 연구개발성과 ... 11
• 3) 목표 달성 수준 ... 13
• 4) 목표 미달 시 원인 분석 ... 13
• 4. 연구개발성과의 관련 분야에 대한 기여 정도(연구개발결과의 중요성) ... 13
• 5. 연구개발성과의 관리 및 활용 계획 ... 13
• 6. 참고문헌 ... 14
• [붙임1] 세부 정량적 연구개발성과 ... 17
• [붙임2] 연구책임자 대표적 연구실적 및 증빙(요약문 및 사본) ... 19
• End of Page ... 23