![CFM treatment negatively regulates adipogenic and lipogenic genes and proteins expression. CFM treatment exerts potent inhibitory effects on differentiation and fat depositions in adipocytes through regulation of key adipogenic factors and their downstream target. a - transcriptional levels of PPAR-γ, C/EBP-β, C/EBP-α FAS, and ACC in experimental cells; b – the translational level of PPAR-γ, C/EBP-α, SREBP-, FAS, ACC and AdipoQ in the experimental cells; c- signaling pathways (pAMPKα, AMPK, pp38, p38, pErk1/2, Erk and GAPDH) involved in control and experimental cells. Gene levels were determined by SYBR green-based RT-PCR method with targeted primers (10pmol each forward and reverse primers). Proteins were detected by Western blot analysis and chemiluminescence method. The data were showed as the mean ± SEM of three independent experiments (n=3) [*] p< 0.05compared to contr](https://nrms.kisti.re.kr/bitextimages/TRKO202300027993/TRKO202300027993_71_image_1.png "CFM treatment negatively regulates adipogenic and lipogenic genes and proteins expression. CFM treatment exerts potent inhibitory effects on differentiation and fat depositions in adipocytes through regulation of key adipogenic factors and their downstream target. a - transcriptional levels of PPAR-γ, C/EBP-β, C/EBP-α FAS, and ACC in experimental cells; b – the translational level of PPAR-γ, C/EBP-α, SREBP-, FAS, ACC and AdipoQ in the experimental cells; c- signaling pathways (pAMPKα, AMPK, pp38, p38, pErk1/2, Erk and GAPDH) involved in control and experimental cells. Gene levels were determined by SYBR green-based RT-PCR method with targeted primers (10pmol each forward and reverse primers). Proteins were detected by Western blot analysis and chemiluminescence method. The data were showed as the mean ± SEM of three independent experiments (n=3) [*] p< 0.05compared to contr")
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| 주관연구기관 | 국립축산과학원 National Institute of Animal Science |
|---|---|
| 연구책임자 | 이기원 |
| 보고서유형 | 최종보고서 |
| 발행국가 | 대한민국 |
| 언어 | 한국어 |
| 발행년월 | 2022-12 |
| 과제시작연도 | 2022 |
| 주관부처 | 농촌진흥청 Rural Development Administration(RDA) |
| 등록번호 | TRKO202300027993 |
| 과제고유번호 | 1395073291 |
| 사업명 | 축산시험연구 |
| DB 구축일자 | 2023-11-02 |
| 키워드 | 고품질.사료작물.사일리지.혼파재배.작부체계.High quality.Forage Crop.Silage.Mixtures.Cropping system. |
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