[보고서](초)미세먼지 유도성 염증 발생과 뇌신경세포 기능저하와의 연계기전 및 개선 기작 연구

허호진

보고서 정보

주관연구기관

경상국립대학교

연구책임자

허호진

보고서유형

최종보고서

발행국가

대한민국

언어

한국어

발행년월

2023-06

과제시작연도

2023

주관부처

교육부
Ministry of Education

연구관리전문기관

한국연구재단
National Research Foundation of Korea

등록번호

TRKO202400004984

과제고유번호

1345365853

사업명

이공학학술연구기반구축

DB 구축일자

2024-09-02

키워드

초/미세먼지.염증발생.인지기능.뇌신경세포.호흡기질환.micro/fine dust.inflammation production.cognitive function.brain neuronal cells.respiratory diseases.

표 Effect of PM2.5 exposure on intracellular ROS contents in A549 cells(A) and MC-IXC (B). Cells were treated with the following PM2.5 concentrations: 25, 50, 100 and 200 μg/mL for 4, 12, 24 and 48 h. Result shown are means ± SD (n = 5)
표 Effect of PM2.5 exposure on cell viability in A549 (A) and MC-IXC (B) cells. Cells were treated with the following PM2.5 concentrations: 25, 50, 100 and 200 μg/mL for 4, 12, 24 and 48 h. Result shown are means ± SD (n = 5)
표 Effect of PM2.5 exposure on DNA damage in A549 (A) and MC-IXC (B) cells. Cells were treated with the following PM2.5 concentrations: 25, 50, 100 and 200 μg/mL for 24 h. Result shown are means ± SD (n = 5). Data were statistically considered at p < 0.05, and different small letters represent statistical difference
표 Protein expression of oxidative stress-related signaling moleculars in PM2.5-treated A549 cells. Band image of western blot analysis (A), p-JNK (B) p-Akt (C) and cleaved caspase-3 (D). Result shown are means ± SD (n = 3). Data were statistically considered at p < 0.05, and different small letters represent statistical differences
표 Protein expression of oxidative stress signaling moleculars in PM2.5-treated MC-IXC cells. Band image of western blot analysis (A), p-JNK (B) p-Akt (C) and cleaved caspase-3 (D). Result shown are means ± SD (n = 3). Data were statistically considered at p < 0.05, and different small letters represent statistical differences
표 Protein expression of cell defence signaling moleculars in PM2.5-treated A549 cells. Band image of western blot analysis (A), Nrf-2 (B) and Keap-1 (C). Result shown are means ± SD (n = 3). Data were statistically considered at p < 0.05, and different small letters represent statistical differences
표 Protein expression of cell defence signaling moleculars in PM2.5-treated MC-IXC cells. Band image of western blot analysis (A), Nrf-2 (B) and Keap-1 (C). Result shown are means ± SD (n = 3). Data were statistically considered at p < 0.05, and different small letters represent statistical differences
표 Intracellular ROS contents (A) and cell viability (B) of various extracts (0 and 80% ethanolic extracts and 4 types of enzyme extracts) from 10 species of marine algae on PM2.5-induced cytotoxicity in A549 cells. The results are expressed in mean ± SD (n = 3). ***p < 0.0001, **p < 0.001, *p < 0.001 versus PM2.5-treated group and using ANOVA followed by a Turkey test
표 Intracellular ROS contents (A) and cell viability (B) of various extracts (0 and 80% ethanolic extracts and 4 types of enzyme extracts) from 10 species of marine algae on PM2.5-induced cytotoxicity in MC-IXC cells. The results are expressed in mean ± SD (n = 3). ***p < 0.0001, **p < 0.001, *p < 0.001 versus PM2.5-treated group and using ANOVA followed by a Turkey test
표 Intracellular ROS content of water and 80% ethanolic extract from Ecklonia cava on PM2.5-induced oxidative stress in RPMI2650 (A), A549 (B) and MC-IXC (C) cells. Data shown were statistically considered at p < 0.05, and different small letters in graph represent statistical difference
표 Cell viability of water and 80% ethanolic extract from Ecklonia cava on PM2.5-induced cytotoxicity in RPMI-2650 (A), A549 (B), and MC-IXC (C) cells. Data shown were statistically considered at p < 0.05, and different small letters in graph represent statistical difference
표 Intracellular ROS contents (A), cell viability (B) and NO contents (C) on PM2.5-induced cytotoxicity in BV-2 cells. Cells were treated with the following PM2.5 concentrations: 1, 2.5, 5, 10, 25, 50 and 100 μg/mL for 4, 12, 24 and 48 h. Result shown are means ± SD (n = 5). Data were statistically considered at p < 0.05, and different small letters represent statistical difference
표 Protein expression of inflammation-mediated signaling moleculars in PM2.5-exposed inflammtion. Band image of westeren blot analysis (A) and relative protein expression (B) in BV-2 cells. Result shown are means ± SD (n = 3). Data were statistically considered at p < 0.05, and different small letters represent statistical differences
표 Protein expression of inflammasome-mediated signaling moleculars in PM2.5-induced inflammation. Band image of westeren blot analysis (A) and relative protein expression (B) in BV-2 cells. Result shown are means ± SD (n = 3). Data were statistically considered at p < 0.05, and different small letters represent statistical differences
표 Cytokine contents on PM2.5-induced inflammation in BV-2 cells. Result shown are means ± SD (n = 3). Data were statistically considered at p < 0.05, and different small letters represent statistical differences
표 Protein expression of inflammasome-mediated signaling moleculars in PM2.5-exposed mice. Band image of western blot analysis (A) and relative protein expression (B) in lung tissues, band image of western blot analysis (C) and relative protein expression (D) in brain tissues. Result shown are means ± SD (n = 5). Data were statistically considered at p < 0.05, and different small letters represent statistical differences
표 Cytokine contents in lung tissues (A) and brain tissues (B) in PM2.5-induced inflammatory models. Result shown are means ± SD (n = 5). Data were statistically considered at p < 0.05, and different small letters represent statistical differences
표 Antioxidant biomarker on PM2.5-induced oxidative stress. SOD activity (A), reduced glutathione (B), and MDA contents (C) in lung tissues. The data are presented as means ± standard deviation (n = 7). Statistical comparisons were performed using the SPSS package for Windows (Version 14). Data were statistically considered at p < 0.05, and different small letters represent statistical differences
표 Mitochondrial function on PM2.5-induced mitochondria damage in lung tissues. Mitochondrial ROS contents (A), mitochondrial membrane potential (MMP) (B), ATP levels (C) in isolated mitochondria from lung tissues. Band images of western blot analysis (D), the protein expression level of p-AMPKα (E), p-Akt (F), Bcl-2 (G), and caspase-3 (H) in lung tissues. The data are presented as means ± standard deviation (n = 5). Statistical comparisons were performed using the SPSS package for Windows (Version 14). Data were statistically considered at p < 0.05, and different small letters represent statistical differences
표 Inflammatory mechanism in brain tissues on PM2.5-induced mice. Band images of western blot analysis (A), the expression level of TLR-4 (B), p-JNK (C), NF-κB (D), p-IκB-α (E), and caspase-1 (F) in lung tissues. The data are presented as means ± standard deviation (n = 5). Statistical comparisons were performed using the SPSS package for Windows (Version 14). Data were statistically considered at p < 0.05, and different small letters represent statistical differences
표 The expression level of pro-inflammatory and anti-inflammatory cytokines on PM2.5-induced inflammation in lung tissues. The expression level of IL-1β (A), IL-6 (B), IL-12 (C), IFN-γ (D), MCP-1 (E), TNF-α (F), IL-4 (G), and IL-10 (H) contents in lung tissues. The data are presented as means ± standard deviation (n = 5). Statistical comparisons were performed using the SPSS package for Windows (Version 14). Data were statistically considered at p < 0.05, and different small letters represent statistical differences
표 The expression level of pro-inflammatory and anti-inflammatory cytokines on PM2.5-induced inflammation in blood serum. The expression level of IL-1β (A), IL-6 (B), IL-12 (C), IFN-γ (D), MCP-1 (E), TNF-α (F), IL-4 (G), and IL-10 (H) contents in serum. The data are presented as means ± standard deviation (n = 5). Statistical comparisons were performed using the SPSS package for Windows (Version 14). Data were statistically considered at p < 0.05, and different small letters represent statistical differences
표 Inflammatory mechanism in brain tissues on PM2.5-induced mice. Band images of western blot analysis (A), the expression level of TLR-4 (B), NF-κB (C), p-IκB-α (D), and caspase-1 (E) in brain tissues. The data are presented as means ± standard deviation (n = 4). Statistical comparisons were performed using the SPSS package for Windows (Version 14). Data were statistically considered at p < 0.05, and different small letters represent statistical differences
표 The expression level of pro-inflammatory and anti-inflammatory cytokines on PM2.5-induced inflammation in brain tissues. The expression level of IL-1β (A), IL-6 (B), IL-12 (C), IFN-γ (D), MCP-1 (E), TNF-α (F), IL-4 (G), and IL-10 (H) contents in brain tissues. The data are presented as means ± standard deviation (n = 4). Statistical comparisons were performed using the SPSS package for Windows (Version 14). Data were statistically considered at p < 0.05, and different small letters represent statistical differences
표 Measurement of metabolite changes in lung tissues on PM2.5-exposed mice using UPLC A-TOF/MS system. PLS-DA score scatter (A) and heat maps of changing molecule profiles (B)
표 Behavioral test on PM2.5-induced learning and memory impairment mice. Total arm entries and alternation behavior (a) and the path tracing of each groups (b) in Y-maze test. Step-through latency time in passive avoidance test (c). The data are presented as means ± standard deviation (n = 7). The results were shown with a mean of ± SD (n = 9). Data were statistically considered at p < 0.05, and different small letters represent statistical difference
표 Mitochondrial function on PM2.5-induced mitochondria damage in brain tissues. Mitochondrial ROS contents (A), mitochondrial membrane potential (MMP) (B), ATP levels (C) in isolated mitochondria from brain tissues. Band images of western blot analysis (D), the protein expression level of p-AMPKα (E), p-Akt (F), Bcl-2 (G), and caspase-3 (H) in brain tissues. The data are presented as means ± standard deviation (n = 5). Statistical comparisons were performed using the SPSS package for Windows (Version 14). Data were statistically considered at p < 0.05, and different small letters represent statistical differences
표 Antioxidant biomarker on PM2.5-induced oxidative stress. SOD activity (A), reduced glutathione level (B), and MDA contents (C) in brain tissues. The data are presented as means ± standard deviation (n = 7). Statistical comparisons were performed using the SPSS package for Windows (Version 14). Data were statistically considered at p < 0.05, and different small letters represent statistical differences
표 Variation in cognitive function-related markers by PM2.5 exposure in brain tissues. Band images of western blot analysis (A), the expression level of p-JNK (B), IDE (C), Aβ (D), and p-Tau (E) in brain tissues. The data are presented as means ± standard deviation (n = 5). Statistical comparisons were performed using the SPSS package for Windows (Version 14). Data were statistically considered at p < 0.05, and different small letters represent statistical differences
표 Variation in cholinergic markers by fine dust (PM2.5) exposure in brain tissues. Band images of western blot analysis (A), the expression level of ChAT (B) and AChR-α3 (C). AChE activity (D) and ACh contents (E) in brain tissues. The data are presented as means ± standard deviation (n = 5). Statistical comparisons were performed using the SPSS package for Windows (Version 14). Data were statistically considered at p < 0.05, and different small letters represent statistical differences
표 Variation in metabolites of kynurenine pathway by fine dust (PM2.5) exposure in brain tissues. Tryptophan (A), kynurenine (B), and kynurenic acid (C) levels, kynurenine/tryptophan (D) and kynurenic acid/kynurenine (E) ratio in brain tissues. The data are presented as means ± standard deviation (n = 7). Statistical comparisons were performed using the SPSS package for Windows (Version 14). Data were statistically considered at p < 0.05, and different small letters represent statistical differences
표 Effect of water extract from Ecklonia cava (WEE) on fine dust (PM2.5) exposure mice. Variations in body weight (A), weight gain (B) and food intake (C) for 12 weeks. The results were shown as means ± SD (n = 14). Data were statistically considered at p < 0.05, and different small letters represent statistical difference
표 Effect of water extract from Ecklonia cava (WEE) on fine dust (PM2.5)-induced learning and memory impairment mice. Total arm entries and alternation behavior (A) and the path tracing of each groups (B) in Y-maze test. Step-through latency time in passive avoidance test (C). Escape latency in the hidden-platform training trial (D), time in W zone for probe trial (E) and path tracing in the probe trial (F) in the Morris water maze test. The results were shown as means ± SD (n = 9). Data were statistically considered at p < 0.05, and different small letters represent statistical difference
표 Mitochondrial activity of water extract from Ecklonia cava (WEE) on fine dust (PM2.5)-induced mitochondria damage in lung tissues. Mitochondrial ROS contents(A), mitochondrial membrane potential (MMP)(B), ATP levels (C) in isolated mitochondria from lung tissues, band images of western blot analysis (D), the protein expression level of mitochondria-related apoptosis (p-AMPKα, p-Akt, Bcl-2, mitochondrial cytochrome C and cleaved caspase-3) (E) in lung tissues. The results were shown as means ± SD (n = 5). Data were statistically considered at p < 0.05, and different small letters represent statistical difference
표 Mitochondrial activity of water extract from Ecklonia cava (WEE) on fine dust (PM2.5)-induced mitochondria damage in brain tissues. Mitochondrial ROS contents (A), mitochondrial membrane potential (MMP) (B), ATP levels (C) in isolated mitochondria from brain tissues, band images of western blot analysis (D), the protein expression level of mitochondria-related apoptosis (p-AMPKα, p-Akt, Bcl-2, mitochondrial cytochrome C and cleaved caspase-3) (E) in brain tissues. The results were shown as means ± SD (n = 5). Data were statistically considered at p < 0.05, and different small letters represent statistical difference
표 Antioxidant effect of water extract from Ecklonia cava (WEE) on fine dust (PM2.5)-induced damage in lung and brain tissues. Superoxide dismutase (SOD) contents (A,B), reduced glutathione (GSH) (C,D), and malondialdehyde (MDA) (E,F). The results were shown as means ± SD (n = 5)
표 Anti-inflammatory effect of water extract from Ecklonia cava (WEE) on fine dust (PM2.5)-induced inflammation in lung tissues. Band images of Western blot analysis (A), the expression level of inflammation-mediated molecules (B) and cytokine content (C) in lung tissues. The results were shown as means ± SD (n = 5). Data were statistically considered at p < 0.05, and different small letters represent statistical difference
표 Anti-inflammatory effect of water extract from Ecklonia cava (WEE) on fine dust (PM2.5)-induced inflammation in brain tissues. Band images of Western blot analysis (A), the expression level of inflammation-mediated molecules (B) and cytokine content (C) in brain tissues. The results were shown as means ± SD (n = 5). Data were statistically considered at p < 0.05, and different small letters represent statistical difference
표 Effect of water extract from Ecklonia cava (WEE) on fine dust (PM2.5)-induced cognitive dysfunction. Band images of Western blot analysis (A) and the expression level of Aβ production/tau hyperphosphorylation-mediated molecules (B) in brain tissues. The results were shown as means ± SD (n = 5). Data were statistically considered at p < 0.05, and different small letters represent statistical difference
표 Effect of water extract from Ecklonia cava (WEE) on fine dust (PM2.5)-induced cholinergic dysfunction. AChE activity (A), band images of Western blot analysis (B) and the expression level (C) of cholinergic system signaling molecules and ACh contents (D) in brain tissues. The results were shown as means ± SD (n = 5). Data were statistically considered at p < 0.05, and different small letters represent statistical difference

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