This study was conducted to investigate the effect of human-derived urine on the acne symptoms of skin conditions. Two samples of 3-7year-old boy's urine was used in freeze-drying after dialysis : one was dialyzed urine in an instant and the other was dialyzed urine that was aged for three days. The subjects were students in their 20s who have acne symptoms; a group of 10 students were selected for the dialyzed urine group (DU), another group of 10 students for the group of the dialyzed after three days urine (DAU) and other group of 10 students for the control group. The experiment had been conducted for a total of 8 weeks. In the results of analysis, in the case of a control group were all increased or not changed. On the other hand, DU group showed decrease in variation of sebum content (40%),amount of keratin (42%), erythema index (7%), blackhead (15%), amount of microorganism (51%) and acne grade level (68%). Besides,DUA group also showed decrease in variation of sebum content (27%), amount of keratin (40%), erythema index (9%), blackhead (16%), amount of microorganism (53%) and acne grade level (61%). Consequently, judging from the changes in the DU group and the DUA group, it was confirmed that the ingredient of urine is very effective in improving acne skin. The results of this research can be used to demonstrate the potentials of urine applicated cosmetics.