The purpose of this study was to examine influence of Opunitia humifusa and MTS (Microneedle therapy system) in the women’s facial skin. The subjects were divided into experimental group (n=14) and control group (n=14). The experimental group was applied Opunitia humifusa and MTS of on facial’s right and control group was offered no management of on facial’s left. Experimental program was held for 30 minutes each time, once a week for eight weeks. Facial skin condition (moisture, sebum, roughness, pore, wrinkle, pigmentation) were analysed during the experiment. A significant increase of skin moisture was found in experimental group (p<.001). Also significant decrease of skin sebum, roughness, pore, wrinkle and pigmentation was found in experimental group (p<.001). But significant improvement of facial skin was not found in control group (p>.05). These result indicate that MTS could bring to desirable change to skin condition. Therefore these extracts from MTS and Opunitia humifusa might be good material for skin care and cosmetic.