A new sensing system based on the immobilization of luminescent bacteria has been proposed for continuous real-time monitoring of pollutants in water or waste water. Photobacterium phosphoreum was used in order to study a response to toxic substances including HgCl2, CdCl2, MnSO4, ZnSO4, FeK3(CN)6, ...
A new sensing system based on the immobilization of luminescent bacteria has been proposed for continuous real-time monitoring of pollutants in water or waste water. Photobacterium phosphoreum was used in order to study a response to toxic substances including HgCl2, CdCl2, MnSO4, ZnSO4, FeK3(CN)6, As2O3, phenol, SeO2 and CrO3 in view of developing monitoring system for toxic substances. In this study the cell concentration of ≥0.7 of O.D660 were selected for the immobilization of P. phosphoreum to have linearity between cell concentrations and bioluminescence intensity. The concentrations of toxic substance causing 50% reduction(EC50) in bioluminescence intensity were determined with both free and immobilized P. phosphoreum. The bioluminescence responses were examined at various concentrations of toxic substance after 10, 20 and 30 min of exposure. The linear correlation between Gamma values and concentrations of toxic substance was obtained and EC50 was calculated from the linear correlation. The respon se curves demonstrate that P. phosphoreum immobilized on the strontium alginate were very sensitive to nine reference chemicals used. The significant inhibitory concentrations for bioluminescence emission were found to be 0.05mg·L-1 for HgCl2, 25 mg·L-1 for CdCl2, 50mg·L-1 for MnSO4, 12.5mg·L-1 for ZnSO4, 100mg·L-1 for FeK3(CN)6, 50mg·L-1 for As2O3, 125mg·L-1 for phenol, 50mg·L-1 for SeO2 and 40mg·L-1 for CrO3, respectively. The free cell and disc type were shown to be more sensitive to toxic substance than cells mixed with sodium alginate or immobilized on strontium alginate. However, the linear regression curves were derived from the strontium alginate immobilized cells was the useful tool for monitoring of toxic substances due to the stability of bioluminescence.
A new sensing system based on the immobilization of luminescent bacteria has been proposed for continuous real-time monitoring of pollutants in water or waste water. Photobacterium phosphoreum was used in order to study a response to toxic substances including HgCl2, CdCl2, MnSO4, ZnSO4, FeK3(CN)6, As2O3, phenol, SeO2 and CrO3 in view of developing monitoring system for toxic substances. In this study the cell concentration of ≥0.7 of O.D660 were selected for the immobilization of P. phosphoreum to have linearity between cell concentrations and bioluminescence intensity. The concentrations of toxic substance causing 50% reduction(EC50) in bioluminescence intensity were determined with both free and immobilized P. phosphoreum. The bioluminescence responses were examined at various concentrations of toxic substance after 10, 20 and 30 min of exposure. The linear correlation between Gamma values and concentrations of toxic substance was obtained and EC50 was calculated from the linear correlation. The respon se curves demonstrate that P. phosphoreum immobilized on the strontium alginate were very sensitive to nine reference chemicals used. The significant inhibitory concentrations for bioluminescence emission were found to be 0.05mg·L-1 for HgCl2, 25 mg·L-1 for CdCl2, 50mg·L-1 for MnSO4, 12.5mg·L-1 for ZnSO4, 100mg·L-1 for FeK3(CN)6, 50mg·L-1 for As2O3, 125mg·L-1 for phenol, 50mg·L-1 for SeO2 and 40mg·L-1 for CrO3, respectively. The free cell and disc type were shown to be more sensitive to toxic substance than cells mixed with sodium alginate or immobilized on strontium alginate. However, the linear regression curves were derived from the strontium alginate immobilized cells was the useful tool for monitoring of toxic substances due to the stability of bioluminescence.
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