천연물로부터 새로운 간보호 활성물질을 개발하기 위해 CCl_4로 흰쥐에 간독성을 유발시킨 후 이에 유용하다고 보고된 26종의 천연물의 효능을 각각 검색하였다. 그 결과 박하, 지유, 괴화, 금은화, ...
천연물로부터 새로운 간보호 활성물질을 개발하기 위해 CCl_4로 흰쥐에 간독성을 유발시킨 후 이에 유용하다고 보고된 26종의 천연물의 효능을 각각 검색하였다. 그 결과 박하, 지유, 괴화, 금은화, 시호, 소계, 동과자, 아가리쿠스버섯, 음양곽, 택사, 독활, 방기, 인동, 자근이 간보호 효과가 있었다. 그 중 간 보호 효과가 강력한 금은화를 대상 약재로 선정하여 70% methanol로 추출하고 효능을 검색하였다. 흰쥐에 CCl_4-Olive(3:2)액 1.0mI/kg(CCl_4로서 0.6ml/kg)의 복강내 투여시에 GPT, G0T, AlP, LDH 활성과 간 중량이 대조군에 비하여 유의성있는 증가를 나타내었고 혈청 중 TG양을 유의적으로 감소시켰다. CCl_4 투여동물에 금은화의 MeOH 추출물 500, 1000mg/kg을 각각 경구투여하였을 때 상기의 효소활성이 대조군에 비하여 유의성 있는 저해를 나타내었으며 간장 중량과 혈중 TG양의 감소를 나타내었다. 따라서 유효 물질을 분리하기 위해 n-hexane, chloroform, ethyl acetate 및 butanol로 계통분획하고 이들 분획에 대하여 간 보호 효능을 다시 검색하였다. 금은화 분획물을 2가지 용량으로 혈액 생화학적 분석을 한 결과 EtOAc, BuOH, 물 분획이 GPT, AlP, LDH 활성을 저해하였으며 이 중 BuOH 분획이 가장 강하게 유의적으로 각 효소의 활성을 저해하였다.
천연물로부터 새로운 간보호 활성물질을 개발하기 위해 CCl_4로 흰쥐에 간독성을 유발시킨 후 이에 유용하다고 보고된 26종의 천연물의 효능을 각각 검색하였다. 그 결과 박하, 지유, 괴화, 금은화, 시호, 소계, 동과자, 아가리쿠스버섯, 음양곽, 택사, 독활, 방기, 인동, 자근이 간보호 효과가 있었다. 그 중 간 보호 효과가 강력한 금은화를 대상 약재로 선정하여 70% methanol로 추출하고 효능을 검색하였다. 흰쥐에 CCl_4-Olive(3:2)액 1.0mI/kg(CCl_4로서 0.6ml/kg)의 복강내 투여시에 GPT, G0T, AlP, LDH 활성과 간 중량이 대조군에 비하여 유의성있는 증가를 나타내었고 혈청 중 TG양을 유의적으로 감소시켰다. CCl_4 투여동물에 금은화의 MeOH 추출물 500, 1000mg/kg을 각각 경구투여하였을 때 상기의 효소활성이 대조군에 비하여 유의성 있는 저해를 나타내었으며 간장 중량과 혈중 TG양의 감소를 나타내었다. 따라서 유효 물질을 분리하기 위해 n-hexane, chloroform, ethyl acetate 및 butanol로 계통분획하고 이들 분획에 대하여 간 보호 효능을 다시 검색하였다. 금은화 분획물을 2가지 용량으로 혈액 생화학적 분석을 한 결과 EtOAc, BuOH, 물 분획이 GPT, AlP, LDH 활성을 저해하였으며 이 중 BuOH 분획이 가장 강하게 유의적으로 각 효소의 활성을 저해하였다.
This study was to study the feasibility of development of a new hepatoprotective substance from natural resources. After developing hepatic intoxication with CCl_4 in rat, screening test was performed for twenty six species of medicinal plants. Each extract was prepared using 95% MeOH and administer...
This study was to study the feasibility of development of a new hepatoprotective substance from natural resources. After developing hepatic intoxication with CCl_4 in rat, screening test was performed for twenty six species of medicinal plants. Each extract was prepared using 95% MeOH and administered orally. It was found that Menthae Herba, Sanguisorba Radix, Sophorae Flos, Lonicerae Flos Lonicerae Folium, Bupleuri Radix, Cersii Herba, Benincasa cerifera, Agaricus Blazei, Epimedii Herba, Alismatis Rhizoma, Araliae cordatae Radix, Sinomeni Caulis et Rhizoma and Lithospermi Radix were effective in the protection of hepatotoxicity. Since among them, Lonicerae Flos was most effective, Lonicera extract was prepared with 70% MeOH and investigated in terms of hepatoprotective effect. By administering the CCl_4 : Olive oil (3:2) at 1.0ml/kg (CCl_4 only 0.6ml/kg) body weight was administered i.p. to experimental animals, GPT, GOT, AlP, LDH activities in the serum and liver weight were increased significantly than the intact group. TG content in the serum was decreased. When Lonicerae Flos extract was given orally at a dose of 1.0g/kg body weight, enzyme activities of the above were inhibited significantly. To investigate the effective components in MeOH extract, Lonicera extract was fractionated systematically by n-hexane, chloroform, ethyl acetate and butanol. Among the fractions EtOAc, BuOH and water fractions inhibited the enzyme activity significantly. Especially BuOH fraction was most efficacious in the inhibitory action against those enzyme activities in the rat serum.
This study was to study the feasibility of development of a new hepatoprotective substance from natural resources. After developing hepatic intoxication with CCl_4 in rat, screening test was performed for twenty six species of medicinal plants. Each extract was prepared using 95% MeOH and administered orally. It was found that Menthae Herba, Sanguisorba Radix, Sophorae Flos, Lonicerae Flos Lonicerae Folium, Bupleuri Radix, Cersii Herba, Benincasa cerifera, Agaricus Blazei, Epimedii Herba, Alismatis Rhizoma, Araliae cordatae Radix, Sinomeni Caulis et Rhizoma and Lithospermi Radix were effective in the protection of hepatotoxicity. Since among them, Lonicerae Flos was most effective, Lonicera extract was prepared with 70% MeOH and investigated in terms of hepatoprotective effect. By administering the CCl_4 : Olive oil (3:2) at 1.0ml/kg (CCl_4 only 0.6ml/kg) body weight was administered i.p. to experimental animals, GPT, GOT, AlP, LDH activities in the serum and liver weight were increased significantly than the intact group. TG content in the serum was decreased. When Lonicerae Flos extract was given orally at a dose of 1.0g/kg body weight, enzyme activities of the above were inhibited significantly. To investigate the effective components in MeOH extract, Lonicera extract was fractionated systematically by n-hexane, chloroform, ethyl acetate and butanol. Among the fractions EtOAc, BuOH and water fractions inhibited the enzyme activity significantly. Especially BuOH fraction was most efficacious in the inhibitory action against those enzyme activities in the rat serum.
AI-Helper
※ AI-Helper는 부적절한 답변을 할 수 있습니다.