A fungus, Penicillium sp. PS-113, with the high phosphate-soluvilizing activities was isolated from Korean soil and formulated to a liquid type with various concentrations of Trio(0, 0.001, 0.01, 0.1 and 1.0%, v/v) and soap(0,0.01, 0.1, 1.0 and 3.0%, w/v) as a dispersing agent. The viability was tes...
A fungus, Penicillium sp. PS-113, with the high phosphate-soluvilizing activities was isolated from Korean soil and formulated to a liquid type with various concentrations of Trio(0, 0.001, 0.01, 0.1 and 1.0%, v/v) and soap(0,0.01, 0.1, 1.0 and 3.0%, w/v) as a dispersing agent. The viability was tested on potato dextrose agar(PDA) medium; that is, the number of Penicillium sp. PS-113 conidia stored at 4℃ and 25℃ for 1 year was couned on PDA at the intervals of 2 weeks. As a result, the liquid formulant containing 0.1%(v/v) Trio was remarkably improved in dispersing ability and the viability of the conidia. In the case of a solid pdwder formulation, several factors for the drying process such as drying temperature, overall drying time, and so on, and humidity of a final product are very important for the conidial viability. The viability of conidia depending on the drying oemperature(60,80,100 and 120℃), humidity(10, 15 and 20%), storage temperature(4 and 25℃) and storage period(0, 0.5,1,2,4,6 and 12 months) was tested on PDA medium during the 1 year storage. As a result, the number of Penicillium sp. PS-113 CONIDIA STORED AT 4℃ after drying at 80℃ was higher than 20~ 60 times to compare with the control. But the viability of the conicia stored after drying at more than 100℃ was dramatically decreased in spite of the periods of storage. Another solid formulants with mycellium, shich was cultivated at 30℃ and 160 rpm in PDB medium, harvested from the submerged liquid culture and then fomulated tp powder type including various clay chemicals such as diatomite, ventonite, talc,kaoline and loess, were tested their viability on PDA medium. As results, the mycellium formulants, stored at 4℃ containing bentonite, diatomite and loess were remarkably imroved their viability upto~85 log% survival ratio during 1 yeay storage, but the control had no counts within 2 months. The phosphate-soluvilizing activity of liquid formulant at the 1 year storage was measured in the presence of insoluble phosphate, 0.5%(w/v) rock phosphate. The fungus, even if 1 year storage, stably produced free phosphate tp the culture broth with the concentration of 340 ppm at the 8 days cultivatiion The effect of the viofertilizer formulated to the liquid type on the growth of plants such as soy bean, mung bean, corn, Chinese cabbage and red bean was evaluated in pot for 2 weeks. As a result, when the pots were included the biofertilizer all plant growths were increased because the phospate-soluvilizing microorganism could continuously supply free phosphate, an essential element for pant growth, to the plant. Quantitative comparison to the control, non-treatment, for growth effect showed not only 1.5 times higher in plant height for soy bean but also 3.4 times root length for mung bean.
A fungus, Penicillium sp. PS-113, with the high phosphate-soluvilizing activities was isolated from Korean soil and formulated to a liquid type with various concentrations of Trio(0, 0.001, 0.01, 0.1 and 1.0%, v/v) and soap(0,0.01, 0.1, 1.0 and 3.0%, w/v) as a dispersing agent. The viability was tested on potato dextrose agar(PDA) medium; that is, the number of Penicillium sp. PS-113 conidia stored at 4℃ and 25℃ for 1 year was couned on PDA at the intervals of 2 weeks. As a result, the liquid formulant containing 0.1%(v/v) Trio was remarkably improved in dispersing ability and the viability of the conidia. In the case of a solid pdwder formulation, several factors for the drying process such as drying temperature, overall drying time, and so on, and humidity of a final product are very important for the conidial viability. The viability of conidia depending on the drying oemperature(60,80,100 and 120℃), humidity(10, 15 and 20%), storage temperature(4 and 25℃) and storage period(0, 0.5,1,2,4,6 and 12 months) was tested on PDA medium during the 1 year storage. As a result, the number of Penicillium sp. PS-113 CONIDIA STORED AT 4℃ after drying at 80℃ was higher than 20~ 60 times to compare with the control. But the viability of the conicia stored after drying at more than 100℃ was dramatically decreased in spite of the periods of storage. Another solid formulants with mycellium, shich was cultivated at 30℃ and 160 rpm in PDB medium, harvested from the submerged liquid culture and then fomulated tp powder type including various clay chemicals such as diatomite, ventonite, talc,kaoline and loess, were tested their viability on PDA medium. As results, the mycellium formulants, stored at 4℃ containing bentonite, diatomite and loess were remarkably imroved their viability upto~85 log% survival ratio during 1 yeay storage, but the control had no counts within 2 months. The phosphate-soluvilizing activity of liquid formulant at the 1 year storage was measured in the presence of insoluble phosphate, 0.5%(w/v) rock phosphate. The fungus, even if 1 year storage, stably produced free phosphate tp the culture broth with the concentration of 340 ppm at the 8 days cultivatiion The effect of the viofertilizer formulated to the liquid type on the growth of plants such as soy bean, mung bean, corn, Chinese cabbage and red bean was evaluated in pot for 2 weeks. As a result, when the pots were included the biofertilizer all plant growths were increased because the phospate-soluvilizing microorganism could continuously supply free phosphate, an essential element for pant growth, to the plant. Quantitative comparison to the control, non-treatment, for growth effect showed not only 1.5 times higher in plant height for soy bean but also 3.4 times root length for mung bean.
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