말굽버섯이 당뇨유발 흰쥐의 혈당, 지질대사 및 면역세포에 미치는 영향 (The) Effect of Fomes fomentarius on Blood Glucose, Lipid Metaboliism and Immune Cell in Steptozotocin-Induced Diabetic Rats원문보기
이 연구는 STZ로 당뇨유발한 흰쥐에서 말굽버섯의 영향에 대하여 조사한 것이다. 당뇨는 Sprague-Dawley계 웅성 흰쥐에 streptozotocin(STZ) 300mg/kg을 0.05M citrate buffer(pH4.5)에 녹여 꼬리정맥으로 투여하여 유발되었다. Sprague-Dawley계 웅성 흰쥐는 하나의 정상 대조군과 다섯군의 STZ 당뇨실험군으로 나뉘어졌다. 당뇨실험군은 당뇨 대조군과 말굽버섯 식이 실험관으로 구분하였다. 모든 군은 일반식이로 하였다. 당뇨 실험군에 각각 물 추출물 100mg/kg과 200mg/kg 그리고 멘탄올 추출물 100mg/kg과 200mg/kg을 자유로이 제공하고 2주간 사육하였다. 체중증가량과 식이섭취량을 측정하고 혈당을 측정하였다. 혈청의 ...
이 연구는 STZ로 당뇨유발한 흰쥐에서 말굽버섯의 영향에 대하여 조사한 것이다. 당뇨는 Sprague-Dawley계 웅성 흰쥐에 streptozotocin(STZ) 300mg/kg을 0.05M citrate buffer(pH4.5)에 녹여 꼬리정맥으로 투여하여 유발되었다. Sprague-Dawley계 웅성 흰쥐는 하나의 정상 대조군과 다섯군의 STZ 당뇨실험군으로 나뉘어졌다. 당뇨실험군은 당뇨 대조군과 말굽버섯 식이 실험관으로 구분하였다. 모든 군은 일반식이로 하였다. 당뇨 실험군에 각각 물 추출물 100mg/kg과 200mg/kg 그리고 멘탄올 추출물 100mg/kg과 200mg/kg을 자유로이 제공하고 2주간 사육하였다. 체중증가량과 식이섭취량을 측정하고 혈당을 측정하였다. 혈청의 알부민, 빌리루빈 함량과 r-GTP, creatinine, BUN을 측정하였다. 혈중 지질농도와 항산화 효소와 보체 단백C_(3)와 혈액성상을 측정하고 백혈구를 측정하고 면역세포인 임파구의 subset을 측정하였다. 체중 증가량은 당뇨대조군과 실험군에서 정상군보다 낮았다. 신장의 무게는 정상군보다 당뇨군에서 높게 나타났으나 말굽버섯 급여로 그 무게가 감소하였다. 비장의 무게는 말굽버섯 멘탄올 추출물 급여군에서 증가를 보였다. 정소상체의 지방량은 모든 실험군에서 당뇨대조군 보다 높게 나타났다. BUN은 모든 실험군에서 당뇨 대조군보다 낮게 나타났다. 빌리루빈량은 멘탄올 추출물 100mg/kg과 200mg/kg에서 유의적인 감소를 나타내었고 r-GTP는 물 추출물 200mg/kg과 멘탄올 추출물 100mg/kg과 200mg/kg에서 유의적인 감소를 나타내었다. 혈당은 말굽버섯 급여로 당뇨대조군에 비하여 유의적으로 혈당강하를 나타내었다. 헤모글로빈은 당뇨군에 영향을 주지 못하였다. 림파구 수치는 메탄올 추출물 100mg/kg에서 유의적인 증가를 나타내었다. 중성지방은 물 추출물 200mg/kg, 멘탄올 추출물 100mg/kg과 200mg/kg에서 유의적인 효과를 나타내었다. Glutathione Peroxidase는 모든 실험군에서 당뇨대조군 보다 높게 나타났다. 보체량에서는 C_(3)의 량이 멘탄올 추출물 100mg/kg과 200mg/kg에서 유의적인 증가를 나타내었다. 면역세포에서는 T cell, B cell, T helper cell 및 T suppresor cell 가 말굽버섯 급여로 당뇨 대조군보다 증가하였다.
이 연구는 STZ로 당뇨유발한 흰쥐에서 말굽버섯의 영향에 대하여 조사한 것이다. 당뇨는 Sprague-Dawley계 웅성 흰쥐에 streptozotocin(STZ) 300mg/kg을 0.05M citrate buffer(pH4.5)에 녹여 꼬리정맥으로 투여하여 유발되었다. Sprague-Dawley계 웅성 흰쥐는 하나의 정상 대조군과 다섯군의 STZ 당뇨실험군으로 나뉘어졌다. 당뇨실험군은 당뇨 대조군과 말굽버섯 식이 실험관으로 구분하였다. 모든 군은 일반식이로 하였다. 당뇨 실험군에 각각 물 추출물 100mg/kg과 200mg/kg 그리고 멘탄올 추출물 100mg/kg과 200mg/kg을 자유로이 제공하고 2주간 사육하였다. 체중증가량과 식이섭취량을 측정하고 혈당을 측정하였다. 혈청의 알부민, 빌리루빈 함량과 r-GTP, creatinine, BUN을 측정하였다. 혈중 지질농도와 항산화 효소와 보체 단백C_(3)와 혈액성상을 측정하고 백혈구를 측정하고 면역세포인 임파구의 subset을 측정하였다. 체중 증가량은 당뇨대조군과 실험군에서 정상군보다 낮았다. 신장의 무게는 정상군보다 당뇨군에서 높게 나타났으나 말굽버섯 급여로 그 무게가 감소하였다. 비장의 무게는 말굽버섯 멘탄올 추출물 급여군에서 증가를 보였다. 정소상체의 지방량은 모든 실험군에서 당뇨대조군 보다 높게 나타났다. BUN은 모든 실험군에서 당뇨 대조군보다 낮게 나타났다. 빌리루빈량은 멘탄올 추출물 100mg/kg과 200mg/kg에서 유의적인 감소를 나타내었고 r-GTP는 물 추출물 200mg/kg과 멘탄올 추출물 100mg/kg과 200mg/kg에서 유의적인 감소를 나타내었다. 혈당은 말굽버섯 급여로 당뇨대조군에 비하여 유의적으로 혈당강하를 나타내었다. 헤모글로빈은 당뇨군에 영향을 주지 못하였다. 림파구 수치는 메탄올 추출물 100mg/kg에서 유의적인 증가를 나타내었다. 중성지방은 물 추출물 200mg/kg, 멘탄올 추출물 100mg/kg과 200mg/kg에서 유의적인 효과를 나타내었다. Glutathione Peroxidase는 모든 실험군에서 당뇨대조군 보다 높게 나타났다. 보체량에서는 C_(3)의 량이 멘탄올 추출물 100mg/kg과 200mg/kg에서 유의적인 증가를 나타내었다. 면역세포에서는 T cell, B cell, T helper cell 및 T suppresor cell 가 말굽버섯 급여로 당뇨 대조군보다 증가하였다.
This study was conducted to investigate the effect of Fomes fomentarius water extracts and methanol extraction diabetic relative biomarkers, reactive blood glucose, lipid level, and immune cell in the streptozotocin-induced diabetic rats. Male Sprague-Dawley rats were divided into normal, diabetic c...
This study was conducted to investigate the effect of Fomes fomentarius water extracts and methanol extraction diabetic relative biomarkers, reactive blood glucose, lipid level, and immune cell in the streptozotocin-induced diabetic rats. Male Sprague-Dawley rats were divided into normal, diabetic control(DM), 100mg/kg water extracts of Fomes fomentarius(DFW100), 200mg/kg water extracts of Fomes fomentarius(DFW200), 100mg/kg methanol extracts of Fomes fomentarius(DFM100) and 200mg/kg methanol extracts of Fomes fomentarius(DFM200) group Diabetes was induced by the injection of streutozotocin in citrate buffer (pH 4.5) into the tail vein at a dose of 300mg/kg of body weight. The extrat was supplemented for 2 weeks. Net weight gain and feed intake were significantly decerased in the diabetic rats. Weight increase was observed in 100mg/kg water and 100mg/kg methanol extracts of Fomes fomentarius group. Relative weight of kidney was increased and those of spleen was significantly decreased in the diabetic control group compared with the normal group. Decreased weight of spleen was improved in the water and methanol extracts of Fomes fomentarius group. Lymphocyte level was significantly decreased in the DM group than the normal groups but WBC was not significantly different in any of the group. Blood glucose level was significantly increased in the DM group than the normal groups but blood glucose level was significantly decreased in the water extracts and methanol of Fomes fomentarius group. Serum bilirubin and γ -GTP content were significantly increased in the DM group compared with the normal group. Increased serum bilirubin and γ -GTP content by STZ were significantly decreased in the water and methanol extracts of Fomes fomentarius group. Serum BUN content was significantly increased in the DM group than those of the normal groups but was significantly decreased in the DFW_(100), DFW_(200) and DFM_(200). Serum TG content were significantly increased in the DM group compared with the normal group, but serum TG content was normalized in the methanol of Fomes fomentarius group. The activatives of SOD and catalase were significantly decreased in diabetic rats. Supplementaion of Fomes fomentarius group were highered their activatives in the diabetic rats. Serum C_(3) was significantly decreased in diabetic rats. In the animals supplemented with Fomes fomentarius, serum C_(3) was significantly increased. The DM group showed decreased T-cell, B-cell, T-helper cell and T-suppres cell. T cell, B cell and T helper cell were incerased in the animals supplemented with Fomes fomentarius These results suggest that Fomes fomentarius was effective on the blood glucose, free radical generation enzyme and immun cell in diabetic conditions.
This study was conducted to investigate the effect of Fomes fomentarius water extracts and methanol extraction diabetic relative biomarkers, reactive blood glucose, lipid level, and immune cell in the streptozotocin-induced diabetic rats. Male Sprague-Dawley rats were divided into normal, diabetic control(DM), 100mg/kg water extracts of Fomes fomentarius(DFW100), 200mg/kg water extracts of Fomes fomentarius(DFW200), 100mg/kg methanol extracts of Fomes fomentarius(DFM100) and 200mg/kg methanol extracts of Fomes fomentarius(DFM200) group Diabetes was induced by the injection of streutozotocin in citrate buffer (pH 4.5) into the tail vein at a dose of 300mg/kg of body weight. The extrat was supplemented for 2 weeks. Net weight gain and feed intake were significantly decerased in the diabetic rats. Weight increase was observed in 100mg/kg water and 100mg/kg methanol extracts of Fomes fomentarius group. Relative weight of kidney was increased and those of spleen was significantly decreased in the diabetic control group compared with the normal group. Decreased weight of spleen was improved in the water and methanol extracts of Fomes fomentarius group. Lymphocyte level was significantly decreased in the DM group than the normal groups but WBC was not significantly different in any of the group. Blood glucose level was significantly increased in the DM group than the normal groups but blood glucose level was significantly decreased in the water extracts and methanol of Fomes fomentarius group. Serum bilirubin and γ -GTP content were significantly increased in the DM group compared with the normal group. Increased serum bilirubin and γ -GTP content by STZ were significantly decreased in the water and methanol extracts of Fomes fomentarius group. Serum BUN content was significantly increased in the DM group than those of the normal groups but was significantly decreased in the DFW_(100), DFW_(200) and DFM_(200). Serum TG content were significantly increased in the DM group compared with the normal group, but serum TG content was normalized in the methanol of Fomes fomentarius group. The activatives of SOD and catalase were significantly decreased in diabetic rats. Supplementaion of Fomes fomentarius group were highered their activatives in the diabetic rats. Serum C_(3) was significantly decreased in diabetic rats. In the animals supplemented with Fomes fomentarius, serum C_(3) was significantly increased. The DM group showed decreased T-cell, B-cell, T-helper cell and T-suppres cell. T cell, B cell and T helper cell were incerased in the animals supplemented with Fomes fomentarius These results suggest that Fomes fomentarius was effective on the blood glucose, free radical generation enzyme and immun cell in diabetic conditions.
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