초록

용어보기논문에서 용어와 풀이말을 자동 추출한 결과로, 시범 서비스 중입니다. AI-Helper

토양으로부터 분리한 방선균중의 한 균주인 Streptomyces sp. HP는 자일로스 (Xylose) 배지에서 높은 역가의 효소를 생산하였다. 밀기울을 산으로 가수분해하여 밀기울중의 자일란 (Xylan) 을 보다 효과적인 효소유발제인 자일로스로 만들었다. 산가수분해한 밀기울을 이용하여 배지의 조성과 배양 조건을 최적화하였다. 또한 이 균주로부터 생산된 ...

토양으로부터 분리한 방선균중의 한 균주인 Streptomyces sp. HP는 자일로스 (Xylose) 배지에서 높은 역가의 효소를 생산하였다. 밀기울을 산으로 가수분해하여 밀기울중의 자일란 (Xylan) 을 보다 효과적인 효소유발제인 자일로스로 만들었다. 산가수분해한 밀기울을 이용하여 배지의 조성과 배양 조건을 최적화하였다. 또한 이 균주로부터 생산된 가용성 효소를 정제하여 여러가지의 효소학적 성질을 조사하였다.

Abstract ▼ AI-Helper

Streptomyces sp. HP produced glucose isomerase(EC 5.3.1. 5 D-xylose ketol-isomerase) and the enzyme yield was high when growth in the medium containing xylose or xylan as carbon source. Xylose was more effective inducer than xylan. By acid hydrolysis of wheat bran, about 70\% of xylan in wheat bran ...

Streptomyces sp. HP produced glucose isomerase(EC 5.3.1. 5 D-xylose ketol-isomerase) and the enzyme yield was high when growth in the medium containing xylose or xylan as carbon source. Xylose was more effective inducer than xylan. By acid hydrolysis of wheat bran, about 70\% of xylan in wheat bran was converted to xylose. By using hydrolyzed wheat bran, the optimal composition of medium was determined. After optimization of medium, the enzyme activity of microbial cells grown in hydrolyzed wheat bran medium was increased four times more than that of microbial cells grown in untreated wheat bran medium. Also some enzymatic properties of soluble enzyme were studied. The enzyme was very stable against heat and had an optimum $p^H$ at 8.0. Michaelis - Menten constants of forward and backward reactions were 0.196M and 0.392M respectively.

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