1. 오염물질의 농도가 생분해에 미치는 영향을 고찰하기 위해 토양에 디젤유의 농도(v/v%)를 1%, 3%, 5%로 달리 오염시킨 후 생분해 실험을 수행결과 1%의 디젤류 농도에서는 초기 투입 14C-Hexadecane 300,000 cpm중 111,636 cpm 이14CO2 로 분해되어 총 37.2%가 실험기간 중 포집 되었으며, 3%, 5%의 디젤유 농도에서는 각각 38,643 cpm, 26,562 cpm 으로 12.2%, 8.6%로, 디젤류의 농도가 증가함에 따라 그 ...
1. 오염물질의 농도가 생분해에 미치는 영향을 고찰하기 위해 토양에 디젤유의 농도(v/v%)를 1%, 3%, 5%로 달리 오염시킨 후 생분해 실험을 수행결과 1%의 디젤류 농도에서는 초기 투입 14C-Hexadecane 300,000 cpm중 111,636 cpm 이14CO2 로 분해되어 총 37.2%가 실험기간 중 포집 되었으며, 3%, 5%의 디젤유 농도에서는 각각 38,643 cpm, 26,562 cpm 으로 12.2%, 8.6%로, 디젤류의 농도가 증가함에 따라 그 분해능이 점차 감소함을 확인 할 수 있었다. 이런 결과로 볼 때 질소원의 첨가가 미생물의 활성도를 높여 더 많은 수의 미생물이 유지되어 미생물의 생장과 질소원의 공급은 밀접한 관계가 있는 것으로 사료된다. 2. 질소원인 N과 오염된 디젤유와의 질량비(C:N)가 100:1 100:5 100:10이 되도록 1%(v/v)의 디젤류로 오염된 토양에 질소원을 첨가하여 약 50일간의 디젤유의 분해양상을 CO₂ 발생량으로 측정한 결과 CO₂발생량이 약 3일정도 지난 후부터 급격히 증가하여 질소원이 첨가되지 않은 초기 투입 14C-Hexadecnae 300,000cpm 중 111,636cpm 이 14CO₂로 분해되어 총37.2%가 실험기간 중 포집 되었으며, 질소 첨가량이 증가 할수록 14CO₂로 포집된양 이 증가 하여 1mM(C:N=100:1), 5mM(C:N=100:5), 10mM(C:N=100:10) 첨가 하였을 경우 각각 139,030(46.3%), 148,010(49.3%), 148,370(49.4%)로 나타났다 이 결과로 볼 때 미생물의 생장과 질소원의 공급이 밀접한 관계가 있는 것으로 판단되며 유류오염 토양을 복원에 영향을 미치는 인자로는 질소원 중요하여 특히 질소원이 첨가 정도에 따라 유류의 분해능에 영향을 미치는 것으로 최적주입량은 어느 정도 차이가 있으나 본 실험에서는 유류는 저 농도에서 영양물질은 질소원 첨가에서 분해정도가 빠르게 나타났고 Control 실험의 경우 분해정도가 완만 곡선을 보였다. 따라서 유류가 난분해성 물질이기는 하나 질소원을 첨가해 주무로서 더 효과적인 토양 복원시킬 수 있을 것으로 사료된다.
1. 오염물질의 농도가 생분해에 미치는 영향을 고찰하기 위해 토양에 디젤유의 농도(v/v%)를 1%, 3%, 5%로 달리 오염시킨 후 생분해 실험을 수행결과 1%의 디젤류 농도에서는 초기 투입 14C-Hexadecane 300,000 cpm중 111,636 cpm 이14CO2 로 분해되어 총 37.2%가 실험기간 중 포집 되었으며, 3%, 5%의 디젤유 농도에서는 각각 38,643 cpm, 26,562 cpm 으로 12.2%, 8.6%로, 디젤류의 농도가 증가함에 따라 그 분해능이 점차 감소함을 확인 할 수 있었다. 이런 결과로 볼 때 질소원의 첨가가 미생물의 활성도를 높여 더 많은 수의 미생물이 유지되어 미생물의 생장과 질소원의 공급은 밀접한 관계가 있는 것으로 사료된다. 2. 질소원인 N과 오염된 디젤유와의 질량비(C:N)가 100:1 100:5 100:10이 되도록 1%(v/v)의 디젤류로 오염된 토양에 질소원을 첨가하여 약 50일간의 디젤유의 분해양상을 CO₂ 발생량으로 측정한 결과 CO₂발생량이 약 3일정도 지난 후부터 급격히 증가하여 질소원이 첨가되지 않은 초기 투입 14C-Hexadecnae 300,000cpm 중 111,636cpm 이 14CO₂로 분해되어 총37.2%가 실험기간 중 포집 되었으며, 질소 첨가량이 증가 할수록 14CO₂로 포집된양 이 증가 하여 1mM(C:N=100:1), 5mM(C:N=100:5), 10mM(C:N=100:10) 첨가 하였을 경우 각각 139,030(46.3%), 148,010(49.3%), 148,370(49.4%)로 나타났다 이 결과로 볼 때 미생물의 생장과 질소원의 공급이 밀접한 관계가 있는 것으로 판단되며 유류오염 토양을 복원에 영향을 미치는 인자로는 질소원 중요하여 특히 질소원이 첨가 정도에 따라 유류의 분해능에 영향을 미치는 것으로 최적주입량은 어느 정도 차이가 있으나 본 실험에서는 유류는 저 농도에서 영양물질은 질소원 첨가에서 분해정도가 빠르게 나타났고 Control 실험의 경우 분해정도가 완만 곡선을 보였다. 따라서 유류가 난분해성 물질이기는 하나 질소원을 첨가해 주무로서 더 효과적인 토양 복원시킬 수 있을 것으로 사료된다.
In the present study, a biodegradation experiment was carried out to investigate the effect of nutrient addition and initial diesel loading rate on the bioremediation of diesel-contaminated soil. Following conclusions were obtained from the results of this study 1. To examine the effects of the init...
In the present study, a biodegradation experiment was carried out to investigate the effect of nutrient addition and initial diesel loading rate on the bioremediation of diesel-contaminated soil. Following conclusions were obtained from the results of this study 1. To examine the effects of the initial diesel concentration on biodegradation, batch biodegradation experiment were conducted by contaminating soil with diesel oil at concentrations of 1%, 3% and 5% (v/v%). According to the result, at diesel oil concentration of 1%, 111,630 cpm out of initial input 300,000 cpm of 14C-Hexadecane was degraded into 14CO₂ and a total of 37.2% was recovered during 60-d of experiment. At diesel oil concentration of 3% and 5%, 38,640 cpm (12.2%) and 26,560 cpm (8.6%) were degraded, respectively, showing that degradability decreases as the diesel concentration increased. Considering these results, the addition of nitrogen source is needed to increase the microbial activity and to maintain a larger number of microbes because the microbial activity is directly related to the supply of nitrogen source. 2. To increase the microbial activity, a nitrogen source was added to soil contaminated with 1% (v/v) diesel oi. The mass ratio of contaminated diesel oil to nitrogen source (C:N ratio) was maintained at 100:1, 100:5 and 100:10, respectively and the biodegradation of diesel oil was conducted for 50 days and the CO₂. production was monitored over time. The results showed that the production of CO₂ increased rapidly from day 3, and of initial input 300,000cpm of 14C-hexadecnae without the addition of nitrogen source 111,630cpm was degraded into 14CO₂, so a total of 37.2% was recovered during the period of experiment. The quantity recovered in the form of 14CO₂ increased with as added nitrogen concentration increased. At nitrogen loading condition of 1mM(C:N=100:1), 5mM(C:N=100:5) and 10mM(C:N=100:10), the recovered volume (14CO₂) was 139,030(46.3%), 148,010(49.3%) and 148,370(49.4%) respectively. This result shows that the microbial activity is in a close relation with the supply of nitrogen source. Nitrogen source is important as a factor affecting the restoration of oil · contaminated soil and particularly the degradability of oil is affected by the addition of nitrogen resource. Although the optimal input varies to some degree, in our study, the degradation of nutrients was accelerated by the addition of nitrogen source at low oil concentration, and the degradability drew a mild curve in the control.
In the present study, a biodegradation experiment was carried out to investigate the effect of nutrient addition and initial diesel loading rate on the bioremediation of diesel-contaminated soil. Following conclusions were obtained from the results of this study 1. To examine the effects of the initial diesel concentration on biodegradation, batch biodegradation experiment were conducted by contaminating soil with diesel oil at concentrations of 1%, 3% and 5% (v/v%). According to the result, at diesel oil concentration of 1%, 111,630 cpm out of initial input 300,000 cpm of 14C-Hexadecane was degraded into 14CO₂ and a total of 37.2% was recovered during 60-d of experiment. At diesel oil concentration of 3% and 5%, 38,640 cpm (12.2%) and 26,560 cpm (8.6%) were degraded, respectively, showing that degradability decreases as the diesel concentration increased. Considering these results, the addition of nitrogen source is needed to increase the microbial activity and to maintain a larger number of microbes because the microbial activity is directly related to the supply of nitrogen source. 2. To increase the microbial activity, a nitrogen source was added to soil contaminated with 1% (v/v) diesel oi. The mass ratio of contaminated diesel oil to nitrogen source (C:N ratio) was maintained at 100:1, 100:5 and 100:10, respectively and the biodegradation of diesel oil was conducted for 50 days and the CO₂. production was monitored over time. The results showed that the production of CO₂ increased rapidly from day 3, and of initial input 300,000cpm of 14C-hexadecnae without the addition of nitrogen source 111,630cpm was degraded into 14CO₂, so a total of 37.2% was recovered during the period of experiment. The quantity recovered in the form of 14CO₂ increased with as added nitrogen concentration increased. At nitrogen loading condition of 1mM(C:N=100:1), 5mM(C:N=100:5) and 10mM(C:N=100:10), the recovered volume (14CO₂) was 139,030(46.3%), 148,010(49.3%) and 148,370(49.4%) respectively. This result shows that the microbial activity is in a close relation with the supply of nitrogen source. Nitrogen source is important as a factor affecting the restoration of oil · contaminated soil and particularly the degradability of oil is affected by the addition of nitrogen resource. Although the optimal input varies to some degree, in our study, the degradation of nutrients was accelerated by the addition of nitrogen source at low oil concentration, and the degradability drew a mild curve in the control.
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