Many perennial plants have been used in the treatment of abdominal pain, hepatitis, chronic gastroenteritis and coughing since ancient times in korea Recently the biological and pharmacological actions of herb have been studied well such as anti-bacterial and anti-tumor activities. Especially Artemi...
Many perennial plants have been used in the treatment of abdominal pain, hepatitis, chronic gastroenteritis and coughing since ancient times in korea Recently the biological and pharmacological actions of herb have been studied well such as anti-bacterial and anti-tumor activities. Especially Artemisia capillaris and Hovenia dulcis are known to have antibacterial effects. In this experiment, Artemisa capillaris and Hovenia dulcis were used in order to investigate the antibacterial activity, when mixed. And we obtained the distilled water fraction, methanol fraction, ethanol fraction, chloroform fraction and ethyl acetate fraction. The mixed ratio were 9:1, 1:1 and 1:9 (Artemisia capillaris : Hovenia dulcis) We used the paper disc diffusion method for measuring inhibition zone and the tryptic soy broth for gaining growth inhibitory curves. In paper disc diffusion method, the results are as follows; In gram positive S. aureus, ethanol fraction 1:1 at a concentration of 0.5mg, ethyl acetate fraction 1:1 at 1mg showed antibacterial activity appearing 26mm(diameter) inhibition zone. L. monocytogenes, ethanol fraction 9:1 at 0.5mg showed antibacterial activity as 40mm inhibition zone and ethyl acetate fraction 9:1 at 0.5mg showed antibacterial activity as 26mm inhibition zone. In gram negative S. enteritidis, ethyl acetate fraction 9:1 at 0.5mg showed antibacterial activity as 30mm inhibition zone, chloroform fraction 1:1 at 0.5mg have an effect of antibacterial activity as 26mm inhibition zone. E. coli, distilled water fraction 9:1, 1:1, 1:9 at 0.5mg showed antibacterial activity as 14mm inhibition zone. In growth inhibitory curves, the results are as follows; In gram positive S. aureus, ethanol fraction 9:1, 1:9, chloroform fraction 9:1, 1:1 and methanol, ethyl acetate fraction 9:1 showed growth inhibitory activities for 72 hours. L. monocytogenes, ethanol fraction 1:9, and methanol, chloroform, ethyl acetate fraction 9:1 showed growth inhibitory activities for 72 hours. In gram negative S. enteritidis, methanol fraction 1:1, 1:9, ethanol fraction 9:1, 1:9 and chloroform, ethyl acetate fraction 9:1 showed growth inhibitory activities for 72 hours, and in E. coli, ethanol fraction 9:1, 1:1, 1:9, chloroform 9:1, 1:1 and methanol, ethyl acetate fraction 9:1 showed growth inhibitory activities for 72 hours. As results of this study, all fractions of 9:1 ratio have the highest antibacterial activity. And ethanol fraction, ethyl acetate fraction, chloroform and methanol fraction have higher antibacterial activity, respectively.
Many perennial plants have been used in the treatment of abdominal pain, hepatitis, chronic gastroenteritis and coughing since ancient times in korea Recently the biological and pharmacological actions of herb have been studied well such as anti-bacterial and anti-tumor activities. Especially Artemisia capillaris and Hovenia dulcis are known to have antibacterial effects. In this experiment, Artemisa capillaris and Hovenia dulcis were used in order to investigate the antibacterial activity, when mixed. And we obtained the distilled water fraction, methanol fraction, ethanol fraction, chloroform fraction and ethyl acetate fraction. The mixed ratio were 9:1, 1:1 and 1:9 (Artemisia capillaris : Hovenia dulcis) We used the paper disc diffusion method for measuring inhibition zone and the tryptic soy broth for gaining growth inhibitory curves. In paper disc diffusion method, the results are as follows; In gram positive S. aureus, ethanol fraction 1:1 at a concentration of 0.5mg, ethyl acetate fraction 1:1 at 1mg showed antibacterial activity appearing 26mm(diameter) inhibition zone. L. monocytogenes, ethanol fraction 9:1 at 0.5mg showed antibacterial activity as 40mm inhibition zone and ethyl acetate fraction 9:1 at 0.5mg showed antibacterial activity as 26mm inhibition zone. In gram negative S. enteritidis, ethyl acetate fraction 9:1 at 0.5mg showed antibacterial activity as 30mm inhibition zone, chloroform fraction 1:1 at 0.5mg have an effect of antibacterial activity as 26mm inhibition zone. E. coli, distilled water fraction 9:1, 1:1, 1:9 at 0.5mg showed antibacterial activity as 14mm inhibition zone. In growth inhibitory curves, the results are as follows; In gram positive S. aureus, ethanol fraction 9:1, 1:9, chloroform fraction 9:1, 1:1 and methanol, ethyl acetate fraction 9:1 showed growth inhibitory activities for 72 hours. L. monocytogenes, ethanol fraction 1:9, and methanol, chloroform, ethyl acetate fraction 9:1 showed growth inhibitory activities for 72 hours. In gram negative S. enteritidis, methanol fraction 1:1, 1:9, ethanol fraction 9:1, 1:9 and chloroform, ethyl acetate fraction 9:1 showed growth inhibitory activities for 72 hours, and in E. coli, ethanol fraction 9:1, 1:1, 1:9, chloroform 9:1, 1:1 and methanol, ethyl acetate fraction 9:1 showed growth inhibitory activities for 72 hours. As results of this study, all fractions of 9:1 ratio have the highest antibacterial activity. And ethanol fraction, ethyl acetate fraction, chloroform and methanol fraction have higher antibacterial activity, respectively.
주제어
#인진쑥 헛개나무 Artemsia capillaris Hovenia dulcis hepatitis
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