The Effects of Polygonum Cuspidatum on the Skin functions Jung Hyun-a Dept. of Oriental Medicine Graduate School, Daejeon University (Directed by Prof. Roh Seok-seon, O.M.D., Ph.D.) Abstract Objectives : This study was carried out to investigate the effects of Polygonum cuspidatum extract on severa...
The Effects of Polygonum Cuspidatum on the Skin functions Jung Hyun-a Dept. of Oriental Medicine Graduate School, Daejeon University (Directed by Prof. Roh Seok-seon, O.M.D., Ph.D.) Abstract Objectives : This study was carried out to investigate the effects of Polygonum cuspidatum extract on several skin functions including inflammation and wrinkle formation. Results : In primary screening of 110 species natural extracts, Polygonum cuspidatum, Chebulae Fructus, Chaenomelis Fructus, and Momordica Cochinchinensis was selected as the potent inhibitors for wrinke-related proteases including collagenase, elastase and gelatinase. In addition, Polygonum cuspidatum extract inhibited the expression of MMP-1, MMP-3 in skin fibroblasts. In in vitro anti-oxidant assay using DPPH method, extracts of Chebulae Fructus and Polygonum cuspidatum showed marked radical scavenging activity. In anti-inflammation assay using macrophage cells, extracts of Chebulae Fructus, Chaenomelis Fructus, Momordica Cochinchinensis and Polygonum cuspidatum showed marked inhibition on LPS-induced NO release. In addition, Polygonum cuspidatum extract inhibited the LPS-induced TNF-α, IL-1β,IL-6, COX-2 expression. In anti-allergy assay using mast cells, Polygonum cuspidatum extract inhibited the PMA and A23187-induced expression of TNF-α and IL-8. In pigmentation assay, Polygonum cuspidatum extract did not show an inhibitory potential on tyrosinase activity and melanin synthesis, indicating that it could not be applicable for skin whitening. In fractionation of Polygonum cuspidatum extract, several secondary metabolites including 2-methpxy-stypandron, physcion, emodin and emodin-6-ο-β-D-glucoside were purified. These compounds also showed mild potency against collagenase, elastase and gelatinase. Especially emodin and emodin-6-ο-β-D-glucoside strongly inhibited collagenase, elastase and gelatinase Conclusion : Polygonum cuspidatum extract has the effects of anti-inflammation and and anti-allergy, which may be due to its inhibitory potential on the macrophage activation. Furthermore, Polygonum cuspidatum extract has the anti-wrinkle effects through the inhibitory potential on the collagnease, elastase and gelatinase activities. These results suggest that Polygonum cuspidatum extract could be applicable for improvement of several skin functions. Keywords : Polygonum cuspidatum extract, anti-oxidant, anti-inflammation, anti-allergy, collagnease, elastase, gelatinase.
The Effects of Polygonum Cuspidatum on the Skin functions Jung Hyun-a Dept. of Oriental Medicine Graduate School, Daejeon University (Directed by Prof. Roh Seok-seon, O.M.D., Ph.D.) Abstract Objectives : This study was carried out to investigate the effects of Polygonum cuspidatum extract on several skin functions including inflammation and wrinkle formation. Results : In primary screening of 110 species natural extracts, Polygonum cuspidatum, Chebulae Fructus, Chaenomelis Fructus, and Momordica Cochinchinensis was selected as the potent inhibitors for wrinke-related proteases including collagenase, elastase and gelatinase. In addition, Polygonum cuspidatum extract inhibited the expression of MMP-1, MMP-3 in skin fibroblasts. In in vitro anti-oxidant assay using DPPH method, extracts of Chebulae Fructus and Polygonum cuspidatum showed marked radical scavenging activity. In anti-inflammation assay using macrophage cells, extracts of Chebulae Fructus, Chaenomelis Fructus, Momordica Cochinchinensis and Polygonum cuspidatum showed marked inhibition on LPS-induced NO release. In addition, Polygonum cuspidatum extract inhibited the LPS-induced TNF-α, IL-1β,IL-6, COX-2 expression. In anti-allergy assay using mast cells, Polygonum cuspidatum extract inhibited the PMA and A23187-induced expression of TNF-α and IL-8. In pigmentation assay, Polygonum cuspidatum extract did not show an inhibitory potential on tyrosinase activity and melanin synthesis, indicating that it could not be applicable for skin whitening. In fractionation of Polygonum cuspidatum extract, several secondary metabolites including 2-methpxy-stypandron, physcion, emodin and emodin-6-ο-β-D-glucoside were purified. These compounds also showed mild potency against collagenase, elastase and gelatinase. Especially emodin and emodin-6-ο-β-D-glucoside strongly inhibited collagenase, elastase and gelatinase Conclusion : Polygonum cuspidatum extract has the effects of anti-inflammation and and anti-allergy, which may be due to its inhibitory potential on the macrophage activation. Furthermore, Polygonum cuspidatum extract has the anti-wrinkle effects through the inhibitory potential on the collagnease, elastase and gelatinase activities. These results suggest that Polygonum cuspidatum extract could be applicable for improvement of several skin functions. Keywords : Polygonum cuspidatum extract, anti-oxidant, anti-inflammation, anti-allergy, collagnease, elastase, gelatinase.
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