미역분말과 fucoxanthin의 보충이 C57BL/6J mouse의 지질대사와 혈당개선에 미치는 영향 Effects of pure fucoxanthin and several types of powdered seaweed on lipid and glucose metabolism in C57BL/6J mouse원문보기
본 연구에서는 자연발생 질환동물 모델로서, 식이 유도성 비만?당뇨 마우스로 널리 사용되어지는 C57BL/6J mouse를 대상으로 fucoxanthin과 다양한 형태의 미역분말의 비만개선 효과 뿐만 아니라, 항당뇨 및 항산화 효과에 걸쳐 다양한 기능을 살펴보고, 건강기능식품소재로서의 응용 가능성에 대하여 검토해 보고자 한다....
본 연구에서는 자연발생 질환동물 모델로서, 식이 유도성 비만?당뇨 마우스로 널리 사용되어지는 C57BL/6J mouse를 대상으로 fucoxanthin과 다양한 형태의 미역분말의 비만개선 효과 뿐만 아니라, 항당뇨 및 항산화 효과에 걸쳐 다양한 기능을 살펴보고, 건강기능식품소재로서의 응용 가능성에 대하여 검토해 보고자 한다.
본 연구에서는 자연발생 질환동물 모델로서, 식이 유도성 비만?당뇨 마우스로 널리 사용되어지는 C57BL/6J mouse를 대상으로 fucoxanthin과 다양한 형태의 미역분말의 비만개선 효과 뿐만 아니라, 항당뇨 및 항산화 효과에 걸쳐 다양한 기능을 살펴보고, 건강기능식품소재로서의 응용 가능성에 대하여 검토해 보고자 한다.
Seaweeds are macroscopic marine algae that form a part of staple diet in Korea and Japan. Fucoxanthin is one of marine carotenoid found in edible brown seaweeds. This study investigated the fucoxanthin and several types of powdereds seaweed regarding blood glucose change and improvement of lipid and...
Seaweeds are macroscopic marine algae that form a part of staple diet in Korea and Japan. Fucoxanthin is one of marine carotenoid found in edible brown seaweeds. This study investigated the fucoxanthin and several types of powdereds seaweed regarding blood glucose change and improvement of lipid and antioxidant metabolism in C57BL/6J mice. After acclimating period for two weeks, five-week-old male C57BL/6J mice were randomly divided into eight groups; normal control(AIN-76 diet), high fat control (total fat 20%), pure fucoxanthin (Fx, 0.02g/100g diet), powered seaweed ethanol extract (EtOH-Sw), enzyme hydrolyzed seaweed powder (Hy-Sw) and ground seaweed powder with 65, 320, 1300 mesh (Gr-Sw). All test substances included in high fat diet are equivalent to 0.02% fucoxanthin. The mice had free access to food and distilled water for nine weeks. The result were as followed; 1. The effect of 0.02% pure fucoxanthin and several types of powdered seaweed with equivalent amount of fucoxanthin on lipid metabolism. The plasma total cholesterol, triglyceride and free fatty acid levels tended to decrease in the experimental groups compared to the high fat control group. Pure Fx and EtOH-Sw supplementation resulted in a significant decrease in the hepatic cholesterol content. In addition the hepatic triglyceride levels were significantly decreased in the experimental groups. The experimental group tended to decrease the hepatic lipogenic enzyme, fatty acid synthase (FAS) activity. The pure Fx and EtOH-Sw supplements remarkably decreased the hepatic β-oxidation activity compared to the high fat control group. Furthermore, 65 and 320 mesh seaweed supplement significantly lowered malic enzyme activities compared to the high fat control group. The phosphatidate phosphohydrolase (PAP) activity was significantly lowered by all supplements given. The hepatic HMG-CoA reductase and ACAT activities were markedly lower in the experimental groups than in the high fat control group. Fecal triglyceride were significantly lower in the pure Fx and Gr-Sw groups than in the high fat control group. 2. The effect of 0.02% pure fucoxanthin and several types of powdered seaweed with equivalent amount of fucoxanthin on glucose metabolism. The 0.02% pure Fx, EtOH-Sw, 320 mesh Gr-Sw supplementation maintained lower blood glucose level compared to the high fat control groups from 6wks over the entired experimental period. However, the plasma insulin and the insulin/glucagon ratio were not different among groups. The plasma adiponectin levels of EtOH-Sw and Hy-Sw groups were significantly higher than those of 0.02% pure Fx and Gr-Sw groups. The glycogen contents were markedly higher in 0.02% pure Fx and 320 mesh Gr-Sw groups than the high fat control group. In analyisis of key enzymes related to hepatic glucose metabolism, glucokinase activity was significantly higher in the 0.02% pure Fx and 320 mesh Gr-Sw groups than in the high fat control group. The hepatic glucose-6-phosphatase (G6Pase) activity was markedly lower in the Hy-Sw and Gr-Sw groups than in the high fat control group, and phosphoenolpyruvate carboxykinase (PEPCK) activity was significantly lower in the experimental groups than in the high fat control group. But the adipocyte PEPCK activities were significantly lower only in the Gr-Sw groups than in the high fat control group. 3. The effect of 0.02% pure fucoxanthin and several types of powdered seaweed with equivalent amount of fucoxanthin on antioxidant metabolism. The experimental groups tended to increase the hepatic catalase (CAT) and glutathione reductase (GR) activities compared to the high fat control group. But the glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) acitivity were not different among groups. The erythrocyte SOD activity was significantly higher in the 0.02% pure Fx, Hy-Sw and 1300 mesh Gr-Sw groups compared to the high fat control group, and the H2O2 levels were markebly lower in the 0.02% pure Fx, EtOH-Sw and 65 mesh Gr-Sw groups than in the high fat control group. Also, thiobarbituric acid reactive substance (TBARS) levels were significantly lower in the experimental groups than in the high fat control group. The hepatic paraoxonase (PON) activity tended to increase the experimental groups compared to the high fat control group, and the plasma PON activity was significantly higher in the experimental groups than in the high fat control group. In conclusion, the data in this study suggest that 0.02% pure fucoxanthin and several types of powdered seaweed with equivalent amount of fucoxanthin improved the hyperglycemia and hyperlipidemia in C57/BL6J mice. First the tested substances were effective to enhance hyperglycemia by decreasing the hepatic glucose output that were mediated via increased glycolysis or decreased gluconeogenesis. Second, these tested substances were beneficial for lowering the hepatic, plasma and fecal lipids by altering the metabolic enzyme activities and fecal lipids extraction. Accordingly, these tested substances including Fx compounds can be used as functional food ingredients for hypolipidemic and blood glucose-lowering action.
Seaweeds are macroscopic marine algae that form a part of staple diet in Korea and Japan. Fucoxanthin is one of marine carotenoid found in edible brown seaweeds. This study investigated the fucoxanthin and several types of powdereds seaweed regarding blood glucose change and improvement of lipid and antioxidant metabolism in C57BL/6J mice. After acclimating period for two weeks, five-week-old male C57BL/6J mice were randomly divided into eight groups; normal control(AIN-76 diet), high fat control (total fat 20%), pure fucoxanthin (Fx, 0.02g/100g diet), powered seaweed ethanol extract (EtOH-Sw), enzyme hydrolyzed seaweed powder (Hy-Sw) and ground seaweed powder with 65, 320, 1300 mesh (Gr-Sw). All test substances included in high fat diet are equivalent to 0.02% fucoxanthin. The mice had free access to food and distilled water for nine weeks. The result were as followed; 1. The effect of 0.02% pure fucoxanthin and several types of powdered seaweed with equivalent amount of fucoxanthin on lipid metabolism. The plasma total cholesterol, triglyceride and free fatty acid levels tended to decrease in the experimental groups compared to the high fat control group. Pure Fx and EtOH-Sw supplementation resulted in a significant decrease in the hepatic cholesterol content. In addition the hepatic triglyceride levels were significantly decreased in the experimental groups. The experimental group tended to decrease the hepatic lipogenic enzyme, fatty acid synthase (FAS) activity. The pure Fx and EtOH-Sw supplements remarkably decreased the hepatic β-oxidation activity compared to the high fat control group. Furthermore, 65 and 320 mesh seaweed supplement significantly lowered malic enzyme activities compared to the high fat control group. The phosphatidate phosphohydrolase (PAP) activity was significantly lowered by all supplements given. The hepatic HMG-CoA reductase and ACAT activities were markedly lower in the experimental groups than in the high fat control group. Fecal triglyceride were significantly lower in the pure Fx and Gr-Sw groups than in the high fat control group. 2. The effect of 0.02% pure fucoxanthin and several types of powdered seaweed with equivalent amount of fucoxanthin on glucose metabolism. The 0.02% pure Fx, EtOH-Sw, 320 mesh Gr-Sw supplementation maintained lower blood glucose level compared to the high fat control groups from 6wks over the entired experimental period. However, the plasma insulin and the insulin/glucagon ratio were not different among groups. The plasma adiponectin levels of EtOH-Sw and Hy-Sw groups were significantly higher than those of 0.02% pure Fx and Gr-Sw groups. The glycogen contents were markedly higher in 0.02% pure Fx and 320 mesh Gr-Sw groups than the high fat control group. In analyisis of key enzymes related to hepatic glucose metabolism, glucokinase activity was significantly higher in the 0.02% pure Fx and 320 mesh Gr-Sw groups than in the high fat control group. The hepatic glucose-6-phosphatase (G6Pase) activity was markedly lower in the Hy-Sw and Gr-Sw groups than in the high fat control group, and phosphoenolpyruvate carboxykinase (PEPCK) activity was significantly lower in the experimental groups than in the high fat control group. But the adipocyte PEPCK activities were significantly lower only in the Gr-Sw groups than in the high fat control group. 3. The effect of 0.02% pure fucoxanthin and several types of powdered seaweed with equivalent amount of fucoxanthin on antioxidant metabolism. The experimental groups tended to increase the hepatic catalase (CAT) and glutathione reductase (GR) activities compared to the high fat control group. But the glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) acitivity were not different among groups. The erythrocyte SOD activity was significantly higher in the 0.02% pure Fx, Hy-Sw and 1300 mesh Gr-Sw groups compared to the high fat control group, and the H2O2 levels were markebly lower in the 0.02% pure Fx, EtOH-Sw and 65 mesh Gr-Sw groups than in the high fat control group. Also, thiobarbituric acid reactive substance (TBARS) levels were significantly lower in the experimental groups than in the high fat control group. The hepatic paraoxonase (PON) activity tended to increase the experimental groups compared to the high fat control group, and the plasma PON activity was significantly higher in the experimental groups than in the high fat control group. In conclusion, the data in this study suggest that 0.02% pure fucoxanthin and several types of powdered seaweed with equivalent amount of fucoxanthin improved the hyperglycemia and hyperlipidemia in C57/BL6J mice. First the tested substances were effective to enhance hyperglycemia by decreasing the hepatic glucose output that were mediated via increased glycolysis or decreased gluconeogenesis. Second, these tested substances were beneficial for lowering the hepatic, plasma and fecal lipids by altering the metabolic enzyme activities and fecal lipids extraction. Accordingly, these tested substances including Fx compounds can be used as functional food ingredients for hypolipidemic and blood glucose-lowering action.
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