Coenzyme Q_(10)은 체내 세포의 에너지를 만들며 강력한 항산화력이 있는 기능성 소재이나 산소와 접촉 시 항산화력을 잃고 물, 알코올에 거의 용해되지 않으므로 coenzyme Q_(10)을 안정하게 생체 내에 효과적으로 전달 및 흡수 될 수 있는 방법을 필요로 하게 되었다. 따라서 본 연구에서는 coenzyme Q_(10)과 유화제를 용매와 혼합한 후, 초고압균질기의 밸브 형태를 달리하여 coenzyme Q_(10)을 30-100 nm 크기로 나노에멀젼화 하고, 제조된 coenzyme Q_(10) 나노에멀젼의 품질 특성 및 저장 안정성 평가를 하여 장기간 보존해도 침전 또는 부유물을 발생시키는 일이 없고, 내산성, 내염성, 내열성 및 동결에도 우수하고 수용액에 대한 ...
Coenzyme Q_(10)은 체내 세포의 에너지를 만들며 강력한 항산화력이 있는 기능성 소재이나 산소와 접촉 시 항산화력을 잃고 물, 알코올에 거의 용해되지 않으므로 coenzyme Q_(10)을 안정하게 생체 내에 효과적으로 전달 및 흡수 될 수 있는 방법을 필요로 하게 되었다. 따라서 본 연구에서는 coenzyme Q_(10)과 유화제를 용매와 혼합한 후, 초고압균질기의 밸브 형태를 달리하여 coenzyme Q_(10)을 30-100 nm 크기로 나노에멀젼화 하고, 제조된 coenzyme Q_(10) 나노에멀젼의 품질 특성 및 저장 안정성 평가를 하여 장기간 보존해도 침전 또는 부유물을 발생시키는 일이 없고, 내산성, 내염성, 내열성 및 동결에도 우수하고 수용액에 대한 분산성 및 투명성이 뛰어난 coenzyme Q_(10) 나노에멀젼을 제조하는데 목적을 두었다. 연구 결과 A, B 밸브에 비해 C 밸브에서 제조한 coenzyme Q_(10) 나노에멀젼 평균 입자가 40 nm로 가장 작았고, 유화 안정성이 가장 높았는데 이는 C 밸브의 구조상 입자들의 충돌 횟수가 많도록 설계되어 입자 크기에 영향을 미치게 된 것으로 사료되었다. 따라서 coenzyme Q_(10) 나노에멀젼 제조를 위한 초고압균질기의 최적 조건은 150 MPa, C 밸브, 통과 횟수 3회인 것으로 나타났다. 초고압균질기의 최적 조건으로 제조된 coenzyme Q_(10) 나노에멀젼의 저장 안정성을 평가하기 위하여 첫번째, 다양한 pH(2-10)와 열(95℃)처리 및 동결(-20℃)처리 후 입자 크기, 투과도 및 제타 전위를 측정하였다. 두번째, coenzyme Q_(10) 나노에멀젼을 12주 동안 4℃, 25℃, 및 40℃에 각각 저장하면서 그 입자 크기, 투과도 및 coenzyme Q_(10) 함량을 측정하였다. 연구 결과, coenzyme Q_(10) 나노에멀젼을 pH(2-10) 용액에 희석하여 열처리 시 pH 4, 6, 8 및 10 용액은 입자 크기와 투과도 값이 균일한 경향을 나타냈으나 pH 2 용액은 1시간 열처리 했을 때, 입자가 커지기 시작하여 7시간 후에는 유화가 깨지는 현상을 확인하였다(p<0.01). 이는 pH 2 용액이 coenzyme Q_(10) 나노에멀젼의 계면막을 파괴하여 입자끼리 응집하여 유화상태가 불안정한 경향을 나타낸 것이라 사료된다. 또한 coenzyme Q_(10) 나노에멀젼을 냉동실에 넣어 -20℃로 유지하며 1, 3, 5, 10, 15 및 30일 마다 입자 크기를 측정한 결과, 10일의 저장 기간 동안은 동결처리 하지 않은 coenzyme Q_(10) 나노에멀젼의 입자 크기와 균일한 경향을 나타냈으나 15일, 30일의 저장기간 동안 동결처리 한 coenzyme Q_(10) 나노에멀젼의 입자 크기는 약간 커지는 경향을 보였다(p<0.01). 하지만 coenzyme Q_(10) 나노에멀젼은 30일 동안 동결처리 후에도 제타 전위 값이 -39 mV에서 -54 mV 범위에 있었음으로 안정하다고 볼 수 있었다. 저장 온도별 실험 결과 4℃와 25℃에서 저장한 coenzyme Q_(10) 나노에멀젼은 12주 동안 입자 크기가 균일하였으나, 40℃에서는 2주 이후 입자가 커지는 경향을 보였고 4주부터는 입자가 급격히 커지며 용액이 불투명해지고, coenzyme Q_(10) 함량이 감소하는 현상이 일어났다(p<0.01). 이 결과로 보아 coenzyme Q_(10) 나노에멀젼은 40℃에서 온도의 영향을 받아 계면막이 파괴되면서 입자끼리 응집하는 속도가 빨라져 입자 크기가 커지고, 가시광선의 한계파장(120 nm)보다 큰 입자 크기로 인해 투과도가 낮은 경향을 나타낸 것이라 사료되며 유화 입자가 깨지면서 coenzyme Q_(10)이 산화되어 coenzyme Q_(10) 함량이 감소하는 현상이 일어났다고 사료된다. 초고압균질기의 최적 조건(150 MPa, C 밸브, 통과 횟수 3회)으로 제조한 coenzyme Q_(10) 나노에멀젼은 평균 입자 크기가 40 nm로 4℃ 및 25℃에서 12주 동안 보존하여도 침전 또는 부유물을 발생시키지 않았고, pH 2 용액을 제외하고는 pH(4-10)처리와 열(95℃)처리 및 동결(-20℃)처리 시, 분산성 및 투명성이 뛰어났음으로 화장품이나 음료에 적용한다면 coenzyme Q_(10)이 피부 및 생체 내에 효과적으로 전달 및 흡수 될 수 있다고 사료된다.
Coenzyme Q_(10)은 체내 세포의 에너지를 만들며 강력한 항산화력이 있는 기능성 소재이나 산소와 접촉 시 항산화력을 잃고 물, 알코올에 거의 용해되지 않으므로 coenzyme Q_(10)을 안정하게 생체 내에 효과적으로 전달 및 흡수 될 수 있는 방법을 필요로 하게 되었다. 따라서 본 연구에서는 coenzyme Q_(10)과 유화제를 용매와 혼합한 후, 초고압균질기의 밸브 형태를 달리하여 coenzyme Q_(10)을 30-100 nm 크기로 나노에멀젼화 하고, 제조된 coenzyme Q_(10) 나노에멀젼의 품질 특성 및 저장 안정성 평가를 하여 장기간 보존해도 침전 또는 부유물을 발생시키는 일이 없고, 내산성, 내염성, 내열성 및 동결에도 우수하고 수용액에 대한 분산성 및 투명성이 뛰어난 coenzyme Q_(10) 나노에멀젼을 제조하는데 목적을 두었다. 연구 결과 A, B 밸브에 비해 C 밸브에서 제조한 coenzyme Q_(10) 나노에멀젼 평균 입자가 40 nm로 가장 작았고, 유화 안정성이 가장 높았는데 이는 C 밸브의 구조상 입자들의 충돌 횟수가 많도록 설계되어 입자 크기에 영향을 미치게 된 것으로 사료되었다. 따라서 coenzyme Q_(10) 나노에멀젼 제조를 위한 초고압균질기의 최적 조건은 150 MPa, C 밸브, 통과 횟수 3회인 것으로 나타났다. 초고압균질기의 최적 조건으로 제조된 coenzyme Q_(10) 나노에멀젼의 저장 안정성을 평가하기 위하여 첫번째, 다양한 pH(2-10)와 열(95℃)처리 및 동결(-20℃)처리 후 입자 크기, 투과도 및 제타 전위를 측정하였다. 두번째, coenzyme Q_(10) 나노에멀젼을 12주 동안 4℃, 25℃, 및 40℃에 각각 저장하면서 그 입자 크기, 투과도 및 coenzyme Q_(10) 함량을 측정하였다. 연구 결과, coenzyme Q_(10) 나노에멀젼을 pH(2-10) 용액에 희석하여 열처리 시 pH 4, 6, 8 및 10 용액은 입자 크기와 투과도 값이 균일한 경향을 나타냈으나 pH 2 용액은 1시간 열처리 했을 때, 입자가 커지기 시작하여 7시간 후에는 유화가 깨지는 현상을 확인하였다(p<0.01). 이는 pH 2 용액이 coenzyme Q_(10) 나노에멀젼의 계면막을 파괴하여 입자끼리 응집하여 유화상태가 불안정한 경향을 나타낸 것이라 사료된다. 또한 coenzyme Q_(10) 나노에멀젼을 냉동실에 넣어 -20℃로 유지하며 1, 3, 5, 10, 15 및 30일 마다 입자 크기를 측정한 결과, 10일의 저장 기간 동안은 동결처리 하지 않은 coenzyme Q_(10) 나노에멀젼의 입자 크기와 균일한 경향을 나타냈으나 15일, 30일의 저장기간 동안 동결처리 한 coenzyme Q_(10) 나노에멀젼의 입자 크기는 약간 커지는 경향을 보였다(p<0.01). 하지만 coenzyme Q_(10) 나노에멀젼은 30일 동안 동결처리 후에도 제타 전위 값이 -39 mV에서 -54 mV 범위에 있었음으로 안정하다고 볼 수 있었다. 저장 온도별 실험 결과 4℃와 25℃에서 저장한 coenzyme Q_(10) 나노에멀젼은 12주 동안 입자 크기가 균일하였으나, 40℃에서는 2주 이후 입자가 커지는 경향을 보였고 4주부터는 입자가 급격히 커지며 용액이 불투명해지고, coenzyme Q_(10) 함량이 감소하는 현상이 일어났다(p<0.01). 이 결과로 보아 coenzyme Q_(10) 나노에멀젼은 40℃에서 온도의 영향을 받아 계면막이 파괴되면서 입자끼리 응집하는 속도가 빨라져 입자 크기가 커지고, 가시광선의 한계파장(120 nm)보다 큰 입자 크기로 인해 투과도가 낮은 경향을 나타낸 것이라 사료되며 유화 입자가 깨지면서 coenzyme Q_(10)이 산화되어 coenzyme Q_(10) 함량이 감소하는 현상이 일어났다고 사료된다. 초고압균질기의 최적 조건(150 MPa, C 밸브, 통과 횟수 3회)으로 제조한 coenzyme Q_(10) 나노에멀젼은 평균 입자 크기가 40 nm로 4℃ 및 25℃에서 12주 동안 보존하여도 침전 또는 부유물을 발생시키지 않았고, pH 2 용액을 제외하고는 pH(4-10)처리와 열(95℃)처리 및 동결(-20℃)처리 시, 분산성 및 투명성이 뛰어났음으로 화장품이나 음료에 적용한다면 coenzyme Q_(10)이 피부 및 생체 내에 효과적으로 전달 및 흡수 될 수 있다고 사료된다.
Coenzyme Q_(10) is a functional material, which creates energy in the body’s cells and has a very strong anti-oxidative power, but loses its anti-oxidative power when it makes contact with oxygen, and is rarely soluble in water or alcohol. Further studies need to be done to make coenzyme Q_(10) deli...
Coenzyme Q_(10) is a functional material, which creates energy in the body’s cells and has a very strong anti-oxidative power, but loses its anti-oxidative power when it makes contact with oxygen, and is rarely soluble in water or alcohol. Further studies need to be done to make coenzyme Q_(10) delivered and absorbed into the body more effectively and with more stability. Therefore, this paper aims at producing coenzyme Q_(10) nanoemulsion, which will not generate deposits or floating matters when preserved for long periods of time, resists acids, bases, heat, and freezing effectively, and easily spreads in an aqueous solution with a higher degree of transparency. This was treated by mixing coenzyme Q_(10), with an emulsifying agent, a solvent, selecting the conditions for nanoemulsion of coenzyme Q_(10)'s size 30-100 nm by using different valve shapes of a high pressure homogenization, and by evaluating the quality characteristics and the storage stability of the produced coenzyme Q_(10) nanoemulsion. As the experiment results, the coenzyme Q_(10) nanoemulsion, produced in the C valve, had the smallest average particle size of 40 nm, and the highest emulsification stability, compared to those in the A and the B valves. This could be due to the fact that the C valve was structure to brought about more collisions among particles and therefore influenced the particle sizes. Therefore, the best high pressure homogenization condition for coenzyme Q_(10) nanoemulsion production was 150 MPa, C valve, and cycled through homogenization three times. In order to evaluate the storage stability of the coenzyme Q_(10) nanoemulsion produced from the best condition for high pressure homogenization, we first measured particle size, transmittance, and zeta potential after various pH (2-10), heat (95℃) and freezing (-20℃) treatments. Second, coenzyme Q_(10) nanoemulsion was stored at 4℃, 25℃, and at 40℃ respectively for 12 weeks, and its particle sizes, transmittance, and coenzyme Q_(10) contents were measured. The study results showed that when coenzyme Q_(10) nanoemulsion was diluted with a solution of pH (2-10) and processed with heat, the solution of pH 4, 6, 8 and 10 tended to cause even particle sizes and transmittance. However, when the solution of pH 2 was processed with heat for one hour, the particles of coenzyme Q_(10) nanoemulsion began to increase in size, and after seven hours, was proved that its emulsification was broken off (p<0.01), demonstrating that the solution of pH 2 destroyed the interface of the coenzyme Q_(10) nanoemulsion, there by causing its particles to condense among themselves, emulsifying and becoming unstable. In addition, when the coenzyme Q_(10) nanoemulsion was stored kept at -20℃ and measuring particle size every 1, 3, 5, 10, 15 and 30 days, the particle size was the same as that of the coenzyme Q_(10) nanoemulsion that was not frozen for ten days of storage, and the particle size of the coenzyme Q_(10) nanoemulsion, which was frozen for 15 days and for 30 days, had a slight increase (p<0.01). The zeta potential value of the coenzyme Q_(10) nanoemulsion was between -39 mV and -54 mV even after 30 days of freezing treatment, even though it was under emulsification, which proved that it was stable. When the experiment was conducted according to the storage temperature, the particle size of the coenzyme Q_(10) nanoemulsion stored at 4℃ and at 25℃, was even for 12 weeks, but had the tendency to increase at 40℃ after two weeks, and after four weeks, its size rapidly increased, the solution became opaque, and the coenzyme Q_(10) contents decreased(p<0.01). The results proved that at 40℃ the interface of the coenzyme Q_(10) nanoemulsion was destroyed because of the temperature, the cohesion among particles speeding up particle size increasing, and with the bigger size of particles than the critical wavelength of a visible ray (120 nm) caused lower transmittance. Also, it was proved that when the emulsification particles were destroyed, the coenzyme Q_(10) was oxidized, and the coenzyme Q_(10) contents decreasing. The coenzyme Q_(10) nanoemulsion, produced from ideal condition of high pressure homogenization (150 MPa, C valve, three times of cycles), had an average size of 40 nm particle size, generated no deposits or floating matters when preserved at 4℃ and at 25℃ for 12 weeks, and displayed excellent dispersibility and transparency when processed with different pH (4-10), heat (95℃), and freezing (-20℃), with the exception of the pH 2 solution. Therefore, when applied to a cosmetic, or a beverage production, coenzyme Q_(10) will be able to transmitted and absorbed into the skin or the body effectively.
Coenzyme Q_(10) is a functional material, which creates energy in the body’s cells and has a very strong anti-oxidative power, but loses its anti-oxidative power when it makes contact with oxygen, and is rarely soluble in water or alcohol. Further studies need to be done to make coenzyme Q_(10) delivered and absorbed into the body more effectively and with more stability. Therefore, this paper aims at producing coenzyme Q_(10) nanoemulsion, which will not generate deposits or floating matters when preserved for long periods of time, resists acids, bases, heat, and freezing effectively, and easily spreads in an aqueous solution with a higher degree of transparency. This was treated by mixing coenzyme Q_(10), with an emulsifying agent, a solvent, selecting the conditions for nanoemulsion of coenzyme Q_(10)'s size 30-100 nm by using different valve shapes of a high pressure homogenization, and by evaluating the quality characteristics and the storage stability of the produced coenzyme Q_(10) nanoemulsion. As the experiment results, the coenzyme Q_(10) nanoemulsion, produced in the C valve, had the smallest average particle size of 40 nm, and the highest emulsification stability, compared to those in the A and the B valves. This could be due to the fact that the C valve was structure to brought about more collisions among particles and therefore influenced the particle sizes. Therefore, the best high pressure homogenization condition for coenzyme Q_(10) nanoemulsion production was 150 MPa, C valve, and cycled through homogenization three times. In order to evaluate the storage stability of the coenzyme Q_(10) nanoemulsion produced from the best condition for high pressure homogenization, we first measured particle size, transmittance, and zeta potential after various pH (2-10), heat (95℃) and freezing (-20℃) treatments. Second, coenzyme Q_(10) nanoemulsion was stored at 4℃, 25℃, and at 40℃ respectively for 12 weeks, and its particle sizes, transmittance, and coenzyme Q_(10) contents were measured. The study results showed that when coenzyme Q_(10) nanoemulsion was diluted with a solution of pH (2-10) and processed with heat, the solution of pH 4, 6, 8 and 10 tended to cause even particle sizes and transmittance. However, when the solution of pH 2 was processed with heat for one hour, the particles of coenzyme Q_(10) nanoemulsion began to increase in size, and after seven hours, was proved that its emulsification was broken off (p<0.01), demonstrating that the solution of pH 2 destroyed the interface of the coenzyme Q_(10) nanoemulsion, there by causing its particles to condense among themselves, emulsifying and becoming unstable. In addition, when the coenzyme Q_(10) nanoemulsion was stored kept at -20℃ and measuring particle size every 1, 3, 5, 10, 15 and 30 days, the particle size was the same as that of the coenzyme Q_(10) nanoemulsion that was not frozen for ten days of storage, and the particle size of the coenzyme Q_(10) nanoemulsion, which was frozen for 15 days and for 30 days, had a slight increase (p<0.01). The zeta potential value of the coenzyme Q_(10) nanoemulsion was between -39 mV and -54 mV even after 30 days of freezing treatment, even though it was under emulsification, which proved that it was stable. When the experiment was conducted according to the storage temperature, the particle size of the coenzyme Q_(10) nanoemulsion stored at 4℃ and at 25℃, was even for 12 weeks, but had the tendency to increase at 40℃ after two weeks, and after four weeks, its size rapidly increased, the solution became opaque, and the coenzyme Q_(10) contents decreased(p<0.01). The results proved that at 40℃ the interface of the coenzyme Q_(10) nanoemulsion was destroyed because of the temperature, the cohesion among particles speeding up particle size increasing, and with the bigger size of particles than the critical wavelength of a visible ray (120 nm) caused lower transmittance. Also, it was proved that when the emulsification particles were destroyed, the coenzyme Q_(10) was oxidized, and the coenzyme Q_(10) contents decreasing. The coenzyme Q_(10) nanoemulsion, produced from ideal condition of high pressure homogenization (150 MPa, C valve, three times of cycles), had an average size of 40 nm particle size, generated no deposits or floating matters when preserved at 4℃ and at 25℃ for 12 weeks, and displayed excellent dispersibility and transparency when processed with different pH (4-10), heat (95℃), and freezing (-20℃), with the exception of the pH 2 solution. Therefore, when applied to a cosmetic, or a beverage production, coenzyme Q_(10) will be able to transmitted and absorbed into the skin or the body effectively.
AI-Helper
※ AI-Helper는 부적절한 답변을 할 수 있습니다.