To investigate the antioxidant effect of Ginseng Radix (GR) on Leydig cells, I was performing cell viability assay using mouse Leydig cellls, TM3. In addition, the protective effects of GR against hydrogen peroxidant-induced oxidant stress in Leydig cells were examined by measuring cell viability un...
To investigate the antioxidant effect of Ginseng Radix (GR) on Leydig cells, I was performing cell viability assay using mouse Leydig cellls, TM3. In addition, the protective effects of GR against hydrogen peroxidant-induced oxidant stress in Leydig cells were examined by measuring cell viability under H2O2 treatment, testosterone level and the antioxidant enzyme levels including SOD and catalase. The following results were obtained.
1. In order to observe the cell viability of GR on TM3 cells, MTT assay was carried out. As a result, GR had no effect on the survival rate of TM3 cells.
2. By measuring the cytotoxicity induced by hydrogen peroxide on TM3 cells, it was found that GR treatment increased survival rate of TM3 at 1, 5 μg/ml of concentration, as of 82.13, 83.20%, respectively.
3. By measuring testosterone level induced by hydrogen peroxide on TM3 cells, it was found that GR treatment increased testosterone level significantly, compared to that of control.
4. By measuring the SOD activity on Leydig cells, the antioxidant effects of GR were decreased, as of 8.04, 7.34, 7.52, 7.16, 6.81 units.
5. By measuring the SOD activity induced by hydrogen peroxide on Leydig cells, the antioxidant effects of GR were increased, compared to that of control.
6. By measuring the catalase activity on Leydig cells, it was found that GR treatment increased the catalase activity, compared to that of control.
7. The catalase activity was increased depending on the concentration of GR. 10 μg/ml of GR treatment showed the most significant protective effect of catalase activity in Leydig cells against H2O2 induced oxidative stress.
In conclusion, the extract of Ginseng Radix has potent antioxidant activity in Leydig cells and it could be used as an antioxidant to protect male reproductive system against oxidative stress.
To investigate the antioxidant effect of Ginseng Radix (GR) on Leydig cells, I was performing cell viability assay using mouse Leydig cellls, TM3. In addition, the protective effects of GR against hydrogen peroxidant-induced oxidant stress in Leydig cells were examined by measuring cell viability under H2O2 treatment, testosterone level and the antioxidant enzyme levels including SOD and catalase. The following results were obtained.
1. In order to observe the cell viability of GR on TM3 cells, MTT assay was carried out. As a result, GR had no effect on the survival rate of TM3 cells.
2. By measuring the cytotoxicity induced by hydrogen peroxide on TM3 cells, it was found that GR treatment increased survival rate of TM3 at 1, 5 μg/ml of concentration, as of 82.13, 83.20%, respectively.
3. By measuring testosterone level induced by hydrogen peroxide on TM3 cells, it was found that GR treatment increased testosterone level significantly, compared to that of control.
4. By measuring the SOD activity on Leydig cells, the antioxidant effects of GR were decreased, as of 8.04, 7.34, 7.52, 7.16, 6.81 units.
5. By measuring the SOD activity induced by hydrogen peroxide on Leydig cells, the antioxidant effects of GR were increased, compared to that of control.
6. By measuring the catalase activity on Leydig cells, it was found that GR treatment increased the catalase activity, compared to that of control.
7. The catalase activity was increased depending on the concentration of GR. 10 μg/ml of GR treatment showed the most significant protective effect of catalase activity in Leydig cells against H2O2 induced oxidative stress.
In conclusion, the extract of Ginseng Radix has potent antioxidant activity in Leydig cells and it could be used as an antioxidant to protect male reproductive system against oxidative stress.
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