법제유황 강화 동충하초 균사체의 생리활성을 평가하고자 in vitro 분석으로 항산화 활성 및 대식세포(Raw 264.7 cell)에서의 세포독성, NO 생성능에 의한 항염증 활성, 인간유래 섬유성육종암세포(HT 1080 cell)에서의 항암활성에 미치는 영향을 분석하였으...
법제유황 강화 동충하초 균사체의 생리활성을 평가하고자 in vitro 분석으로 항산화 활성 및 대식세포(Raw 264.7 cell)에서의 세포독성, NO 생성능에 의한 항염증 활성, 인간유래 섬유성육종암세포(HT 1080 cell)에서의 항암활성에 미치는 영향을 분석하였으며, in vivo 분석으로 STZ로 유발된 당뇨쥐의 혈당 및 지질대사, 간 기능에 미치는 영향을 조사한 결과 법제유황의 약리적 효능을 가진 동충하초 균사체는 항산화 활성에서 법제유황 무 첨가군에 비해 다소 낮은 활성을 보였으나, 대식세포의 세포독성 및 NO 생성능, 항암활성에서는 법제유황을 첨가한 군에서 유용한 효과가 있음을 확인하였으며, in vivo 실험에서 역시 항당뇨 효과가 있음을 알 수 있었다. 또한 법제유황의 독성에 대한 안전성도 일부 확인하였으며, 향후 지속적인 실험과 면밀한 연구 검토 후 법제유황의 다양한 활용이 가능할 것으로 판단된다.
법제유황 강화 동충하초 균사체의 생리활성을 평가하고자 in vitro 분석으로 항산화 활성 및 대식세포(Raw 264.7 cell)에서의 세포독성, NO 생성능에 의한 항염증 활성, 인간유래 섬유성육종암세포(HT 1080 cell)에서의 항암활성에 미치는 영향을 분석하였으며, in vivo 분석으로 STZ로 유발된 당뇨쥐의 혈당 및 지질대사, 간 기능에 미치는 영향을 조사한 결과 법제유황의 약리적 효능을 가진 동충하초 균사체는 항산화 활성에서 법제유황 무 첨가군에 비해 다소 낮은 활성을 보였으나, 대식세포의 세포독성 및 NO 생성능, 항암활성에서는 법제유황을 첨가한 군에서 유용한 효과가 있음을 확인하였으며, in vivo 실험에서 역시 항당뇨 효과가 있음을 알 수 있었다. 또한 법제유황의 독성에 대한 안전성도 일부 확인하였으며, 향후 지속적인 실험과 면밀한 연구 검토 후 법제유황의 다양한 활용이 가능할 것으로 판단된다.
The purpose of this study was evaluated physiological activity of Cordyceps militaris mycelium with processed sulfur. Proliferation of processed sulfur (PS) with Cordyceps militaris mycelium was increased in dose-dependent manner. In organic sulfur contents of Cordyceps militalis mycelium fortified ...
The purpose of this study was evaluated physiological activity of Cordyceps militaris mycelium with processed sulfur. Proliferation of processed sulfur (PS) with Cordyceps militaris mycelium was increased in dose-dependent manner. In organic sulfur contents of Cordyceps militalis mycelium fortified processed sulfur, CM+PSH (CM+3000 ppm of PS) was significantly higher than other groups. However, CM+PSL (CM+1500 ppm of PS) was almost changed organic sulfur. Content of total polyphenol compounds was similarity to CM, CM+PSL and CM+PSH. The EDA (electron donating ability) and SOD-like activity was increased in dose-dependent manner and the activity of CM were significantly higher than CM+PSL and CM+PSH. We examined cytotoxicity, nitric oxide production of Raw 264.7 cell and inhibition of HT 1080 cell by MTT assay. CM, CM+PSL and CM+PSH do not have any toxic effects in macrophages (Raw 264.7). And CM+PSL and CM+PSH inhibited the production of nitrite in Raw 264.7 cells activated with LPS. The antitumor effects of Cordyceps militaris mycelium with processed sulfur on HT 1080 cell was indicated a significantly inhibition activity. To evaluate of hypo-glycemic effects in vivo, we examined blood glucose levels, serum lipid profile and hepatotoxicity of streptozotocin (STZ) induced diabetic rats. Hypo-glycemic effect of CM, CM+PSL and CM+PSH groups was significantly higher than DC groups. The activities of ALT, AST and ALP in CM+PSH group were lower than DC, PC groups. These results suggested that Cordyceps militaris mycelium with processed sulfur have activities of antioxidant, antitumor and hypo-glycemic effects.
The purpose of this study was evaluated physiological activity of Cordyceps militaris mycelium with processed sulfur. Proliferation of processed sulfur (PS) with Cordyceps militaris mycelium was increased in dose-dependent manner. In organic sulfur contents of Cordyceps militalis mycelium fortified processed sulfur, CM+PSH (CM+3000 ppm of PS) was significantly higher than other groups. However, CM+PSL (CM+1500 ppm of PS) was almost changed organic sulfur. Content of total polyphenol compounds was similarity to CM, CM+PSL and CM+PSH. The EDA (electron donating ability) and SOD-like activity was increased in dose-dependent manner and the activity of CM were significantly higher than CM+PSL and CM+PSH. We examined cytotoxicity, nitric oxide production of Raw 264.7 cell and inhibition of HT 1080 cell by MTT assay. CM, CM+PSL and CM+PSH do not have any toxic effects in macrophages (Raw 264.7). And CM+PSL and CM+PSH inhibited the production of nitrite in Raw 264.7 cells activated with LPS. The antitumor effects of Cordyceps militaris mycelium with processed sulfur on HT 1080 cell was indicated a significantly inhibition activity. To evaluate of hypo-glycemic effects in vivo, we examined blood glucose levels, serum lipid profile and hepatotoxicity of streptozotocin (STZ) induced diabetic rats. Hypo-glycemic effect of CM, CM+PSL and CM+PSH groups was significantly higher than DC groups. The activities of ALT, AST and ALP in CM+PSH group were lower than DC, PC groups. These results suggested that Cordyceps militaris mycelium with processed sulfur have activities of antioxidant, antitumor and hypo-glycemic effects.
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