홍삼의 법제유황 처리가 당뇨쥐의 혈중지질 및 대사지표물질에 미치는 영향 Effect of Red Ginseng with processed sulfur extracts on serum lipids concentration and metabolic variables in diabetic rats원문보기
광물성한약재인 유황의 활용방안을 다각화하기 위한 연구의 일환으로 法製하지 않은 生硫黃과 방법을 달리해 法製한 유황을 항당뇨효과가 뛰어나다고 알려진 식물성 약재인 紅蔘과 함께 추출한 후 생리활성과 streptozotocin을 이용해 당뇨를 유발한 쥐의 혈당, 혈중지질, 간독성 등 체내에 미치는 영향을 조사하여 다음과 같은 결과를 얻었다. 동일량의 홍삼에 동일량의 유황을 첨가했음에도 法製한 유황의 첨가가 조사포닌의 함량과 황함량을 높이는 것으로 나타났다. ...
광물성한약재인 유황의 활용방안을 다각화하기 위한 연구의 일환으로 法製하지 않은 生硫黃과 방법을 달리해 法製한 유황을 항당뇨효과가 뛰어나다고 알려진 식물성 약재인 紅蔘과 함께 추출한 후 생리활성과 streptozotocin을 이용해 당뇨를 유발한 쥐의 혈당, 혈중지질, 간독성 등 체내에 미치는 영향을 조사하여 다음과 같은 결과를 얻었다. 동일량의 홍삼에 동일량의 유황을 첨가했음에도 法製한 유황의 첨가가 조사포닌의 함량과 황함량을 높이는 것으로 나타났다. 항산화활성에 대한 실험결과 法製유황의 첨가가 총폴리페놀 화합물의 함량에는 변화를 주지 않았으나 전자공여능과 SOD 유사활성에서는 法製한 유황을 첨가한 추출물이 그렇지 않은 추출물에 비해 우수했다. Streptozotocin으로 유발한 당뇨쥐의 혈당은 GPSⅡ군의 실험기간중의 평균혈당이 DC군보다 18.34% 낮게 나타났으며 혈당변화경향도 안정적이었으나 GS군과 GPSⅠ군은 DC군에 비해선 평균혈당이 낮으나 GPSⅡ군에 비해서는 떨어졌다. 간기능과 신장기능관련 지표물질의 측정결과 AST와 ALP는 DC군이 가장 높게 나타난데 비해 GPSⅡ군이 다른 실험군들에 비해 낮게 나타났고, BUN 또한 GPSⅡ군이 NC군에 비해서는 높으나 다른 당뇨유발군들에 비해 낮게 나타나 당뇨에 의한 손상은 있었으나 法製유황의 內服에 따른 특이적 독성반응은 없는 것으로 나타났다 이상의 결과로 法製硫黃의 홍삼과의 合用이 당뇨유발 동물의 지질과 대사지표물질에 대해 개선효과를 가진다고 판단되며 향후 法製 유황의 內服에 따르는 치료효과와 독성반응에 대한 연구의 기초자료로 삼을 수 있을 것으로 판단된다.
광물성한약재인 유황의 활용방안을 다각화하기 위한 연구의 일환으로 法製하지 않은 生硫黃과 방법을 달리해 法製한 유황을 항당뇨효과가 뛰어나다고 알려진 식물성 약재인 紅蔘과 함께 추출한 후 생리활성과 streptozotocin을 이용해 당뇨를 유발한 쥐의 혈당, 혈중지질, 간독성 등 체내에 미치는 영향을 조사하여 다음과 같은 결과를 얻었다. 동일량의 홍삼에 동일량의 유황을 첨가했음에도 法製한 유황의 첨가가 조사포닌의 함량과 황함량을 높이는 것으로 나타났다. 항산화활성에 대한 실험결과 法製유황의 첨가가 총폴리페놀 화합물의 함량에는 변화를 주지 않았으나 전자공여능과 SOD 유사활성에서는 法製한 유황을 첨가한 추출물이 그렇지 않은 추출물에 비해 우수했다. Streptozotocin으로 유발한 당뇨쥐의 혈당은 GPSⅡ군의 실험기간중의 평균혈당이 DC군보다 18.34% 낮게 나타났으며 혈당변화경향도 안정적이었으나 GS군과 GPSⅠ군은 DC군에 비해선 평균혈당이 낮으나 GPSⅡ군에 비해서는 떨어졌다. 간기능과 신장기능관련 지표물질의 측정결과 AST와 ALP는 DC군이 가장 높게 나타난데 비해 GPSⅡ군이 다른 실험군들에 비해 낮게 나타났고, BUN 또한 GPSⅡ군이 NC군에 비해서는 높으나 다른 당뇨유발군들에 비해 낮게 나타나 당뇨에 의한 손상은 있었으나 法製유황의 內服에 따른 특이적 독성반응은 없는 것으로 나타났다 이상의 결과로 法製硫黃의 홍삼과의 合用이 당뇨유발 동물의 지질과 대사지표물질에 대해 개선효과를 가진다고 판단되며 향후 法製 유황의 內服에 따르는 치료효과와 독성반응에 대한 연구의 기초자료로 삼을 수 있을 것으로 판단된다.
The purpose of this study was expanded application of processed sulfur for internal medicine and evaluation of effects by processed methods in extracts with red ginseng. To evaluate biological activity, we prepared red ginseng with non processed sulfur extracts(GS), red ginseng with processed sulfur...
The purpose of this study was expanded application of processed sulfur for internal medicine and evaluation of effects by processed methods in extracts with red ginseng. To evaluate biological activity, we prepared red ginseng with non processed sulfur extracts(GS), red ginseng with processed sulfurⅠextracts(GPSⅠ) and red ginseng with processed sulfurⅡextracts(GPSⅡ). examined contents of crude saponin, SOD-like activity, contents of total polyphenol compounds, electron donating ability and α-glucosidase inhibitory activity. Contents of crude saponin increased by processed sulfur, and GPSⅡ was shown highest contents in crude saponin and sulfur compared with another groups. Total polyphenol compounds was non-significantly difference in each groups but electron donating ability of GPSⅡ was shown highest activity compared with GS and GPSⅠ, SOD-like activity showed same tendency as electron donating ability at 1 ㎎/㎖concentration. Inhibitory activity of α-glucosidase was approximately same level in acarbose and GPSⅡ, another extracts decreased activity compared with acarbose. To evaluate of hypoglycemic activity in vivo, we examined blood glucose level, serum lipid profile and metabolic variables of streptozotocin(STZ) induced diabetic rat. Experimental group was divided into 5 groups : first, it was divided into normal control group(hereafter NC group) and diabetes-induced group, and diabetes induced groups was divided into diabetic control group(DC group)，treated with 300 mg/kg body weight of GS, GPSⅠ and GPSⅡ extracts groups, respectively. Blood glucose level of GPSⅡ group was decreased 18.34% compared with DC group and maintained stability range in glucose level. but GS and GPSⅠshowed high level compared to GPSⅡ. In serum lipid concentration, also showed lowest level in GPSⅡ. The concentration of total cholesterol was no significantly difference in each groups. The activity of ALT, AST and ALP was shown high level in diabetic induced groups, and lowest level in GPSⅡ, but was not shown specific toxicity by processed sulfur administration. Creatinine was shown non-significantly difference in each groups and GPSⅡ was shown lowest level in BUN. These result expressed red ginseng with processed sulfur have improvement effects on diabetes and internal medicinal application of processed sulfur have no specific toxicity in liver and kidney. and suggested that it might be a basic data about processed sulfur.
The purpose of this study was expanded application of processed sulfur for internal medicine and evaluation of effects by processed methods in extracts with red ginseng. To evaluate biological activity, we prepared red ginseng with non processed sulfur extracts(GS), red ginseng with processed sulfurⅠextracts(GPSⅠ) and red ginseng with processed sulfurⅡextracts(GPSⅡ). examined contents of crude saponin, SOD-like activity, contents of total polyphenol compounds, electron donating ability and α-glucosidase inhibitory activity. Contents of crude saponin increased by processed sulfur, and GPSⅡ was shown highest contents in crude saponin and sulfur compared with another groups. Total polyphenol compounds was non-significantly difference in each groups but electron donating ability of GPSⅡ was shown highest activity compared with GS and GPSⅠ, SOD-like activity showed same tendency as electron donating ability at 1 ㎎/㎖concentration. Inhibitory activity of α-glucosidase was approximately same level in acarbose and GPSⅡ, another extracts decreased activity compared with acarbose. To evaluate of hypoglycemic activity in vivo, we examined blood glucose level, serum lipid profile and metabolic variables of streptozotocin(STZ) induced diabetic rat. Experimental group was divided into 5 groups : first, it was divided into normal control group(hereafter NC group) and diabetes-induced group, and diabetes induced groups was divided into diabetic control group(DC group)，treated with 300 mg/kg body weight of GS, GPSⅠ and GPSⅡ extracts groups, respectively. Blood glucose level of GPSⅡ group was decreased 18.34% compared with DC group and maintained stability range in glucose level. but GS and GPSⅠshowed high level compared to GPSⅡ. In serum lipid concentration, also showed lowest level in GPSⅡ. The concentration of total cholesterol was no significantly difference in each groups. The activity of ALT, AST and ALP was shown high level in diabetic induced groups, and lowest level in GPSⅡ, but was not shown specific toxicity by processed sulfur administration. Creatinine was shown non-significantly difference in each groups and GPSⅡ was shown lowest level in BUN. These result expressed red ginseng with processed sulfur have improvement effects on diabetes and internal medicinal application of processed sulfur have no specific toxicity in liver and kidney. and suggested that it might be a basic data about processed sulfur.
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