비파잎(Eriobotrya japonica Lindl.) 추출물의 여드름균에 대한 항균효과와 항산화 활성 Antimicrobial Effects on Acne and Antioxidant Activities of Extract from Leaves of Eriobotrya japonica Lindl원문보기
비파잎을 건조 후 95% 에탄올과 증류수를 용매로 사용하여 온도별로 추출하고, 추출물의 함유된 폴리페놀과 플라보노이드의 함량을 HPLC로 분석하였으며 여드름균에 대한 항균효과를 측정하였다. 에탄올을 이용한 비파잎의 추출수율이 증류수의 수율보다 높게 나타났으며 polyphenol acid류인 ...
비파잎을 건조 후 95% 에탄올과 증류수를 용매로 사용하여 온도별로 추출하고, 추출물의 함유된 폴리페놀과 플라보노이드의 함량을 HPLC로 분석하였으며 여드름균에 대한 항균효과를 측정하였다. 에탄올을 이용한 비파잎의 추출수율이 증류수의 수율보다 높게 나타났으며 polyphenol acid류인 tannin acid, caffeic acid, 3,5-dihydroxybenzoic acid가 95% 에탄올 및 증류수 추출물에서 모두 분석되었다. 프리라디칼 소거효과는 60℃에탄올, 99℃증류수추출 조건에서 대조군인 BHT(butylated hydroxytoluene)보다 항산화 효과가 높게 나타났다. 여드름균인 Propionibacteriun acne, Pseudomonas aeruginosa에 대한 항균 효과는 미미하였지만, Staphylococcus epidermidis, Staphylococcus aureus, Pityrosporum ovale은 microbroth dilution의 실험을 통해 항균 효과를 보였다. 추출물의 최소살균농도는 1.0∼1.5%로 합성 방부제인 propyl paraben, methyl paraben과 유사한 살균력을 나타내었다.
비파잎을 건조 후 95% 에탄올과 증류수를 용매로 사용하여 온도별로 추출하고, 추출물의 함유된 폴리페놀과 플라보노이드의 함량을 HPLC로 분석하였으며 여드름균에 대한 항균효과를 측정하였다. 에탄올을 이용한 비파잎의 추출수율이 증류수의 수율보다 높게 나타났으며 polyphenol acid류인 tannin acid, caffeic acid, 3,5-dihydroxybenzoic acid가 95% 에탄올 및 증류수 추출물에서 모두 분석되었다. 프리라디칼 소거효과는 60℃에탄올, 99℃증류수추출 조건에서 대조군인 BHT(butylated hydroxytoluene)보다 항산화 효과가 높게 나타났다. 여드름균인 Propionibacteriun acne, Pseudomonas aeruginosa에 대한 항균 효과는 미미하였지만, Staphylococcus epidermidis, Staphylococcus aureus, Pityrosporum ovale은 microbroth dilution의 실험을 통해 항균 효과를 보였다. 추출물의 최소살균농도는 1.0∼1.5%로 합성 방부제인 propyl paraben, methyl paraben과 유사한 살균력을 나타내었다.
Leaves extracts of Eriobotrya japonica Lindl. were prepared using distilled water and alcohol as a solvent at various temperature. The contents of polyphenols and flavonoids were assayed with HPLC analysis. Antimicrobial effects of leaves extracts of Eriobotrya japonica Lindl. against acne-inducing ...
Leaves extracts of Eriobotrya japonica Lindl. were prepared using distilled water and alcohol as a solvent at various temperature. The contents of polyphenols and flavonoids were assayed with HPLC analysis. Antimicrobial effects of leaves extracts of Eriobotrya japonica Lindl. against acne-inducing bacteria had been tested. The yield of watery extract was larger than that of alcoholic extracts and polyphenolic acids such as tannin acid, caffeic acid, 3,5-dihydroxybenzoic acid were detected on both extracts. The free radical scavenging capacity of alcohol extract(60℃) was bigger than that of BHT(butylated hydroxytoluene) and distilled water extract(90℃) was equivalent to capacity of alcohol extract(60℃). In microbroth dilution method, antimicrobial effect of both extracts against Propionibacteriun acne and Pseudomonas aeruginosa was trivial but antimicrobial effect of both extracts against Staphylococcus epidermidis, Staphylococcus aureus, Pityrosporum ovale had been shown. In the concentration of extracts from 1.0 to 1.5%, the sterilizing power of both extracts was similar to that of synthetic preservative such as methyl paraben, propyl paraben.
Leaves extracts of Eriobotrya japonica Lindl. were prepared using distilled water and alcohol as a solvent at various temperature. The contents of polyphenols and flavonoids were assayed with HPLC analysis. Antimicrobial effects of leaves extracts of Eriobotrya japonica Lindl. against acne-inducing bacteria had been tested. The yield of watery extract was larger than that of alcoholic extracts and polyphenolic acids such as tannin acid, caffeic acid, 3,5-dihydroxybenzoic acid were detected on both extracts. The free radical scavenging capacity of alcohol extract(60℃) was bigger than that of BHT(butylated hydroxytoluene) and distilled water extract(90℃) was equivalent to capacity of alcohol extract(60℃). In microbroth dilution method, antimicrobial effect of both extracts against Propionibacteriun acne and Pseudomonas aeruginosa was trivial but antimicrobial effect of both extracts against Staphylococcus epidermidis, Staphylococcus aureus, Pityrosporum ovale had been shown. In the concentration of extracts from 1.0 to 1.5%, the sterilizing power of both extracts was similar to that of synthetic preservative such as methyl paraben, propyl paraben.
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