애기장대(A. thaliana) NIT1 유전자와 보리 Dhn5 유전자의 abiotic 스트레스 저항성 관련 기능 분석 Functional analysis of Arabidopsis NIT1 and Barley Dhn5 in abiotic stress tolerance원문보기
In order to cope with environmental fluctuations, plants have developed strategies to adapt to stress by a number of physiological and biochemical modifications, reflected at the level of gene expression. We identified the NIT1(Newly Identified Transcript1) which is a novel gene predicted in the ann...
In order to cope with environmental fluctuations, plants have developed strategies to adapt to stress by a number of physiological and biochemical modifications, reflected at the level of gene expression. We identified the NIT1(Newly Identified Transcript1) which is a novel gene predicted in the annotated region of the Arabidopsis genome, chromosome 1. The presence of the putative cis-acting elements, ABRE, DRE and MYC which are involved in abiotic stress expression in promoter indicates that NIT1 is induced or up-regulated by abiotic stress. When the NIT1 was expressed in cold-sensitive E. coli BX04, the transformed bacteria was grown more faster than that of the control under cold growth condition. To research the physiological function of the NIT1 gene, the seeds of Arabidopsis Col-0, the nit1 mutant, the nit1 transgenic plant, the NIT1 transgenic plant were germinated on the MS media or pot and grown to osmotic, salt, drought, cold and freezing stress conditions. The nit1 transgenic plant secured the tolerance of abiotic stresses and the extremely resistant in the other plants. The NIT1 transgenic plant is more tolerant than wild type to abiotic stress conditions. The nit1 mutant is more sensitive than wild type at the germination rate and the growth to osmotic and salt stress. In the case of drought, the survival rate of nit1 mutant is half of wild type. When the germination and seedlings growth of the plants were assessed at low temperature, no differences were observed. But then nit1 mutant is more sensitive than wild type to freezing stress. ontributed theConsequently, the NIT1 is c abiotic stress tolerance to plants. The NIT is localized in the nucleus. To study the physiological function of the Dhn5, the Dhn5 was also introduced into cold-sensitive E. coli BX04 and checked growth rate at cold shock condition. the Dhn5 transformant was grown similar to positive control, the CspA transformant. This result indicates that Dhn5 involved in cold stress tolerance mechanism.
In order to cope with environmental fluctuations, plants have developed strategies to adapt to stress by a number of physiological and biochemical modifications, reflected at the level of gene expression. We identified the NIT1(Newly Identified Transcript1) which is a novel gene predicted in the annotated region of the Arabidopsis genome, chromosome 1. The presence of the putative cis-acting elements, ABRE, DRE and MYC which are involved in abiotic stress expression in promoter indicates that NIT1 is induced or up-regulated by abiotic stress. When the NIT1 was expressed in cold-sensitive E. coli BX04, the transformed bacteria was grown more faster than that of the control under cold growth condition. To research the physiological function of the NIT1 gene, the seeds of Arabidopsis Col-0, the nit1 mutant, the nit1 transgenic plant, the NIT1 transgenic plant were germinated on the MS media or pot and grown to osmotic, salt, drought, cold and freezing stress conditions. The nit1 transgenic plant secured the tolerance of abiotic stresses and the extremely resistant in the other plants. The NIT1 transgenic plant is more tolerant than wild type to abiotic stress conditions. The nit1 mutant is more sensitive than wild type at the germination rate and the growth to osmotic and salt stress. In the case of drought, the survival rate of nit1 mutant is half of wild type. When the germination and seedlings growth of the plants were assessed at low temperature, no differences were observed. But then nit1 mutant is more sensitive than wild type to freezing stress. ontributed theConsequently, the NIT1 is c abiotic stress tolerance to plants. The NIT is localized in the nucleus. To study the physiological function of the Dhn5, the Dhn5 was also introduced into cold-sensitive E. coli BX04 and checked growth rate at cold shock condition. the Dhn5 transformant was grown similar to positive control, the CspA transformant. This result indicates that Dhn5 involved in cold stress tolerance mechanism.
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