Clostridium botulinum type A toxin purified through DEAE-Sepharose, Heparin-Sepharose, superose-6, and SP Sepharose was repeatedly administered to pregnant rats. Intramuscular administration was done at the early organogenesis period (5 to 16 days of pregnancy). Pregnant rats administered with more ...
Clostridium botulinum type A toxin purified through DEAE-Sepharose, Heparin-Sepharose, superose-6, and SP Sepharose was repeatedly administered to pregnant rats. Intramuscular administration was done at the early organogenesis period (5 to 16 days of pregnancy). Pregnant rats administered with more than 1 U/kg/day resulted in paralytic gait, consistent inhibition of weight increase, and decrease in corrected body weight and weight gain. In addition, administration of more than 4 U/kg/day resulted in decreased feed consumption and decreased weights of thymus, spleen, kidneys, heart, lung, and liver. Meanwhile , the decreased embryo weights (both male and female embryos) and the increased number of dwarf embryos were observed when more than 1 U/kg/day was administered. On administration of more than 4 U/kg/day, the decreased placental weights and the decreased number of metatarsi were found. Accordingly, it is considered that NOAEL of Clostridium botulinum type A toxin for pregnant rats and embryos might be not more than 1 U/kg/day. Further, This toxin may not be teratogenic, but delayed ossification characterized by the decreased number of metatarsi was observed at high dose group, which is a secondary consequence of maternal toxicity. Moreover, it is considered that changes to organ weights observed in ICR mice administered with test article might not be resulted from the toxin itself. Rather, such changes are considered to naturally occur during pregnancy. Further studies on resistance to toxin after neurotomy are required.
Clostridium botulinum type A toxin purified through DEAE-Sepharose, Heparin-Sepharose, superose-6, and SP Sepharose was repeatedly administered to pregnant rats. Intramuscular administration was done at the early organogenesis period (5 to 16 days of pregnancy). Pregnant rats administered with more than 1 U/kg/day resulted in paralytic gait, consistent inhibition of weight increase, and decrease in corrected body weight and weight gain. In addition, administration of more than 4 U/kg/day resulted in decreased feed consumption and decreased weights of thymus, spleen, kidneys, heart, lung, and liver. Meanwhile , the decreased embryo weights (both male and female embryos) and the increased number of dwarf embryos were observed when more than 1 U/kg/day was administered. On administration of more than 4 U/kg/day, the decreased placental weights and the decreased number of metatarsi were found. Accordingly, it is considered that NOAEL of Clostridium botulinum type A toxin for pregnant rats and embryos might be not more than 1 U/kg/day. Further, This toxin may not be teratogenic, but delayed ossification characterized by the decreased number of metatarsi was observed at high dose group, which is a secondary consequence of maternal toxicity. Moreover, it is considered that changes to organ weights observed in ICR mice administered with test article might not be resulted from the toxin itself. Rather, such changes are considered to naturally occur during pregnancy. Further studies on resistance to toxin after neurotomy are required.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.