Contact dermatitis is a term for a skin reaction resulting from exposure to allergens. Irritant dermatitis is either acute or chronic, which is usually associated with strong and weak irritants respectively. In an acute irritant dermatitis model, mice were treated by topical application of 1% 2,4-di...
Contact dermatitis is a term for a skin reaction resulting from exposure to allergens. Irritant dermatitis is either acute or chronic, which is usually associated with strong and weak irritants respectively. In an acute irritant dermatitis model, mice were treated by topical application of 1% 2,4-dinitrochlorobenzene (DNCB) on the dorsum of both ears on day 0. Sophoricoside was injected intraperitoneally at doses of 3, 10, and 30 mg/kg on day 0. In chronic irritant dermatitis model, mice were treated by topical application of 1% DNCB on the dorsum of both ears daily for three consecutive days from day 0. Sophoricoside was injected intraperitoneally at doses of 3, 10, and 30 mg/kg daily for three consecutive days from day 0. Allergic contact dermatitis (ACD), one form of the delayed-type hypersensitivities, is a common occupational and environmental health issue. In an ACD model, mice were sensitized by topical painting on the abdomen with 1% DNCB on day 0 and day 5. Mice were induced by the topical application of 1% DNCB on the dorsum of both ears. Sophoricoside was injected intraperitoneally at doses of 3, 10, and 30 mg/kg daily for four consecutive days from day 10. It was founded that this compound was effective in prevention of sensitization or elicitation skin inflammation induced by DNCB. Sophoricoside prevented DNCB-induced enlargement of auricular lymph nodes and inflammatory cytokine production of lymph node cells, suggesting that sophoricoside inhibited skin lesion via inhibiting DNCB-induced abnormal immune responses. By using in vitro cellular assays, it was proved that sophoricoside strongly inhibited proliferation of B cells, modelately T cell proliferation, but not immune functions of macrophages and dendritic cells. Furthermore, sophoricoside interfered nuclear translocation of NF-κB p65 and phosphorylation/degradation of IκBα/β in LPS-activated B cells. Sophoricoside did not affect phosphorylation of p38, ERK, and JNK in LPS-activated B cells. In summary, the present study shows that sophoricoside ameliorates ACD via inhibiting NF-κB signaling pathway in B cells, and suggest that this compound may be useful for the treatment of patients with ACD.
Contact dermatitis is a term for a skin reaction resulting from exposure to allergens. Irritant dermatitis is either acute or chronic, which is usually associated with strong and weak irritants respectively. In an acute irritant dermatitis model, mice were treated by topical application of 1% 2,4-dinitrochlorobenzene (DNCB) on the dorsum of both ears on day 0. Sophoricoside was injected intraperitoneally at doses of 3, 10, and 30 mg/kg on day 0. In chronic irritant dermatitis model, mice were treated by topical application of 1% DNCB on the dorsum of both ears daily for three consecutive days from day 0. Sophoricoside was injected intraperitoneally at doses of 3, 10, and 30 mg/kg daily for three consecutive days from day 0. Allergic contact dermatitis (ACD), one form of the delayed-type hypersensitivities, is a common occupational and environmental health issue. In an ACD model, mice were sensitized by topical painting on the abdomen with 1% DNCB on day 0 and day 5. Mice were induced by the topical application of 1% DNCB on the dorsum of both ears. Sophoricoside was injected intraperitoneally at doses of 3, 10, and 30 mg/kg daily for four consecutive days from day 10. It was founded that this compound was effective in prevention of sensitization or elicitation skin inflammation induced by DNCB. Sophoricoside prevented DNCB-induced enlargement of auricular lymph nodes and inflammatory cytokine production of lymph node cells, suggesting that sophoricoside inhibited skin lesion via inhibiting DNCB-induced abnormal immune responses. By using in vitro cellular assays, it was proved that sophoricoside strongly inhibited proliferation of B cells, modelately T cell proliferation, but not immune functions of macrophages and dendritic cells. Furthermore, sophoricoside interfered nuclear translocation of NF-κB p65 and phosphorylation/degradation of IκBα/β in LPS-activated B cells. Sophoricoside did not affect phosphorylation of p38, ERK, and JNK in LPS-activated B cells. In summary, the present study shows that sophoricoside ameliorates ACD via inhibiting NF-κB signaling pathway in B cells, and suggest that this compound may be useful for the treatment of patients with ACD.
주제어
#Sophoricoside Allergic contact dermatitis B cells NF-κB
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