[논문]Protective Effect of Fisetin (3,7,3',4'-Tetrahydroxyflavone) against γ-Irradiation-Induced Oxidative Stress and Cell Damage

Piao, Mei Jing; Kim, Ki Cheon; Chae, Sungwook; Keum, Young Sam; Kim, Hye Sun; Hyun, Jin Won

doi:10.4062/biomolther.2013.017

Protective Effect of Fisetin (3,7,3',4'-Tetrahydroxyflavone) against γ-Irradiation-Induced Oxidative Stress and Cell Damage 원문보기

Biomolecules & therapeutics, v.21 no.3, 2013년, pp.210 - 215

Piao, Mei Jing (School of Medicine and Institute for Nuclear Science and Technology, Jeju National University) , Kim, Ki Cheon (School of Medicine and Institute for Nuclear Science and Technology, Jeju National University) , Chae, Sungwook (Aging Research Center, Korea Institute of Oriental Medicine) , Keum, Young Sam (Department of Biochemistry, College of Pharmacy, Dongguk University) , Kim, Hye Sun (Cancer Research Institute, Seoul National University College of Medicine) , Hyun, Jin Won (School of Medicine and Institute for Nuclear Science and Technology, Jeju National University)

Abstract ▼ AI-Helper

Ionizing radiation can induce cellular oxidative stress through the generation of reactive oxygen species, resulting in cell damage and cell death. The aim of this study was to determine whether the antioxidant effects of the flavonoid fisetin (3,7,3',4'-tetrahydroxyflavone) included the radioprotection of cells exposed to ${\gamma}$-irradiation. Fisetin reduced the levels of intracellular reactive oxygen species generated by ${\gamma}$-irradiation and thereby protected cells against ${\gamma}$-irradiation-induced membrane lipid peroxidation, DNA damage, and protein carbonylation. In addition, fisetin maintained the viability of irradiated cells by partially inhibiting ${\gamma}$-irradiation-induced apoptosis and restoring mitochondrial membrane potential. These effects suggest that the cellular protective effects of fisetin against ${\gamma}$-irradiation are mainly due to its inhibition of reactive oxygen species generation.

주제어

AI 본문요약
AI-Helper

* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.

제안 방법

In this study, fisetin was found to inhibit DNA damage in cells exposed to γ-irradiation and to protect cell membrane lipids and cellular proteins from radiation-induced peroxidation and carbonylation, respectively.
Thus, this study investigated the ability of fisetin to inhibit cell damage in Chinese hamster lung fibroblasts exposed to γ-irradiation, and the underlying mechanisms conferring protection.

데이터처리

The data were subjected to an analysis of variance (ANOVA) using Tukey’s test to analyze differences.

이론/모형

Twenty-four hours later, DCF-DA was added to each well to a final concentration of 100 μM and cells were incubated for an additional 30 min at 37°C. After washing with phosphate-buffered saline (PBS), the stained cells were mounted in mounting medium (DAKO, Carpinteria, CA, USA) on a microscope slide and were then observed by confocal microscopy, and images were collected using the Laser Scanning Microscope 5 PASCAL program (Carl Zeiss, Jena, Germany).
The effect of fisetin on cell viability was determined in a [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium] bromide (MTT) assay, which is based on the reduction of a tetrazolium salt by mitochondrial dehydrogenase in viable cells (Carmichael et al., 1987). Cells were treated (or not) with fisetin and subjected to γ-irradiation as described above.

참고문헌 (34)

1 Arai Y. Watanabe S. Kimira M. Shimoi K. Mochizuki R. Kinae N. Dietary intakes of flavonols, flavones and isofl avones by Japanese women and the inverse correlation between quercetin intake and plasma LDL cholesterol concentration. J. Nutr. (2000) 130 2243 2250 10958819

상세보기
2 Beecher G. R. Overview of dietary flavonoids: nomenclature, occurrence and intake. J. Nutr. (2003) 133 3248S 3254S 14519822

상세보기
3 Cai J. Yang J. Jones D. P. Mitochondrial control of apoptosis: the role of cytochrome c. Biochim. Biophys. Acta (1998) 1366 139 149 10.1016/S0005-2728(98)00109-1 9714780

상세보기
4 Carmichael J. DeGraff W. G. Gazdar A. F. Minna J. D. Mitchell J. B. Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Res. (1987) 47 936 942 3802100

상세보기
5 Cossarizza A. Baccarani-Contri M. Kalashnikova G. Franceschi C. A new method for the cytofl uorimetric analysis of mitochondrial membrane potential using the J-aggregate forming lipophilic cation 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimidaz olcarbocyanine iodide (JC-1). Biochem. Biophys. Res. Commun. (1993) 197 40 45 10.1006/bbrc.1993.2438 8250945

상세보기
6 Dubner D. Gisone P. Jaitavich I. Perez M. Free radicals production and estimation of oxidative stress related to gamma irradiation. Biol. Trace Elem. Res. (1995) 47 265 270 10.1007/BF02790126 7779556

상세보기
7 Fotsis T. Pepper M. S. Montesano R. Aktas E. Breit S. Schweigerer L. Rasku S. Wähälä K. Adlercreutz H. Phytoestrogens and inhibition of angiogenesis. Baillieres Clin. Endocrinol. Metab. (1998) 12 649 666 10.1016/S0950-351X(98)80009-8 10384818

상세보기
8 Gudkov S. V. Shtarkman I. N. Smirnova V. S. Chernikov A. V. Bruskov V. I. Guanosine and inosine display antioxidant activity, protect DNA in vitro from oxidative damage induced by reactive oxygen species, and serve as radioprotectors in mice. Radiat. Res. (2006) 165 538 545 10.1667/RR3552.1 16669708

상세보기
9 Hanneken A. Lin F. F. Johnson J. Maher P. Flavonoids protect human retinal pigment epithelial cells from oxidative-stress induced death. Invest. Ophthalmol. Vis. Sci. (2006) 47 3164 3177 10.1167/iovs.04-1369 16799064

상세보기
10 Halliwell B. Gutteridge J. M. C. Oxidative stress: adaptation, damage, repair and death. In Free Radicals in Biology and Medicine (B. Halliwell and J.M.C. Gutteridge, Eds.) Oxford University Oxford (1999) 3rd ed. 246 350
11 Ishige K. Schubert D. Sagara Y. Flavonoids protect neuronal cells from oxidative stress by three distinct mechanisms. Free Radic. Biol. Med. (2001) 30 433 446 10.1016/S0891-5849(00)00498-6 11182299

상세보기
12 Kim S. Y. Seo M. Oh J. M. Cho E. A. Juhnn Y. S. Inhibition of gamma ray-induced apoptosis by stimulatory heterotrimeric GTP binding protein involves Bcl-xL downregulation in SH-SY5Y human neuroblastoma cells. Exp. Mol. Med. (2007) 39 583 593 10.1038/emm.2007.64 18059134

상세보기
13 Lee J. H. Kim S. Y. Kil I. S. Park J. W. Regulation of ionizing radiation-induced apoptosis by mitochondrial NADP+-dependent isocitrate dehydrogenase. J. Biol. Chem. (2007) 282 13385 13394 10.1074/jbc.M700303200 17350954

상세보기
14 Maher P. Akaishi T. Abe K. Flavonoid fisetin promotes ERK dependent long-term potentiation and enhances memory. Proc. Natl. Acad. Sci. U.S.A. (2006) 103 16568 16573 10.1073/pnas.0607822103 17050681

상세보기
15 Middleton E. Jr Kandaswami C. Effects of flavonoids on immune and inflammatory cell functions. Biochem. Pharmacol. (1992) 43 1167 1179 10.1016/0006-2952(92)90489-6 1562270

상세보기
16 Middleton E. Jr Kandaswami C. Theoharides T. C. The effects of plant flavonoids on mammalian cells: implications for inflammation, heart disease, and cancer. Pharmacol. Rev. (2000) 52 673 751 11121513

상세보기
17 Ohkawa H. Ohishi N. Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem. (1979) 95 351 358 10.1016/0003-2697(79)90738-3 36810

상세보기
18 Okimoto Y. Watanabe A. Niki E. Yamashita T. Noguchi N. A novel fluorescent probe diphenyl-1-pyrenylphosphine to follow lipid peroxidation in cell membranes. FEBS Lett. (2000) 474 137 140 10.1016/S0014-5793(00)01587-8 10838073

상세보기
19 Rajagopalan R. Ranjan S. K. Nair C. K. Effect of vinblastine sulfate on gamma-radiation-induced DNA single-strand breaks in murine tissues. Mutat. Res. (2003) 536 15 25 10.1016/S1383-5718(03)00015-9 12694742

상세보기
20 Reers M. Smith T. W. Chen L. B. J-aggregate formation of a carbocyanine as a quantitative fluorescent indicator of membrane potential. Biochemistry (1991) 30 4480 4486 10.1021/bi00232a015 2021638

상세보기
21 Regula K. M. Ens K. Kirshenbaum L. A. Mitochondriaassisted cell suicide: a license to kill. J. Mol. Cell Cardiol. (2003) 35 559 567 10.1016/S0022-2828(03)00118-4 12788372

상세보기
22 Riley P. A. Free radicals in biology: oxidative stress and the effects of ionizing radiation. Int. J. Radiat. Biol. (1994) 65 27 33 10.1080/09553009414550041 7905906

상세보기
23 Rosenkranz A. R. Schmaldienst S. Stuhlmeier K. M. Chen W. Knapp W. Zlabinger G. J. A microplate assay for the detection of oxidative products using 2′,7′-dichlorofl uorescein-diacetate. J. Immunol. Methods (1992) 156 39 45 10.1016/0022-1759(92)90008-H 1431161

상세보기
24 Sies H. In Oxidative Stress (H. Sies, Ed.). Academic Press New York (1983)
25 Simon H. U. Haj-Yehia A. Levi-Schaffer F. Role of reactive oxygen species (ROS) in apoptosis induction. Apoptosis (2000) 5 415 418 10.1023/A:1009616228304 11256882

상세보기
26 Singh N. P. Microgels for estimation of DNA strand breaks, DNA protein cross links and apoptosis. Mutat. Res. (2000) 455 111 127 10.1016/S0027-5107(00)00075-0 11113471

상세보기
27 Spitz D. R. Azzam E. I. Li J. J. Gius D. Metabolic oxidation/ reduction reactions and cellular responses to ionizing radiation: A unifying concept in stress response biology. Cancer Metastasis Rev. (2004) 23 311 322 10.1023/B:CANC.0000031769.14728.bc 15197331

상세보기
28 Stadtman E. R. Oxidation of free amino acids and amino acid residues in proteins by radiolysis and by metal-catalyzed reactions. Annu. Rev. Biochem. (1993) 62 797 821 10.1146/annurev.bi.62.070193.004053 8352601

상세보기
29 Sung B. Pandey M. K. Aggarwal B. B. Fisetin, an inhibitor of cyclindependent kinase 6, down-regulates nuclear factorkappaB- regulated cell proliferation, antiapoptotic and metastatic gene products through the suppression of TAK-1 and receptor interacting protein-regulated IkappaBalpha kinase activation. Mol. Pharmacol. (2007) 71 1703 1714 10.1124/mol.107.034512 17387141

상세보기
30 Troiano L. Ferraresi R. Lugli E. Nemes E. Roat E. Nasi M. Pinti M. Cossarizza A. Multiparametric analysis of cells with different mitochondrial membrane potential during apoptosis by polychromatic flow cytometry. Nat. Protoc. (2007) 2 2719 2727 10.1038/nprot.2007.405 18007607

상세보기
31 Wang L. Tu Y. C. Lian T. W. Hung J. T. Yen J. H. Wu M. J. Distinctive antioxidant and antiinfl ammatory effects of flavonols. J. Agric. Food Chem. (2006) 54 9798 9804 10.1021/jf0620719 17177504

상세보기
32 Zamzami N. Marchetti P. Castedo M. Zanin C. Vayssiere J. L. Petit P. X. Kroemer G. Reduction in mitochondrial potential constitutes an early irreversible step of programmed lymphocyte death in vivo. J. Exp. Med. (1995) 181 1661 1672 10.1084/jem.181.5.1661 7722446

상세보기
33 Zamzami N. Susin S. A. Marchetti P. Hirsch T. Gomez-Monterrey I. Castedo M. Kroemer G. Mitochondrial control of nuclear apoptosis. J. Exp. Med. (1996) 183 1533 1544 10.1084/jem.183.4.1533 8666911

상세보기
34 Zbarsky V. Datla K. P. Parkar S. Rai D. K. Aruoma O. I. Dexter D. T. Neuroprotective properties of the natural phenolic antioxidants curcumin and naringenin but not quercetin and fisetin in a 6-OHDA model of Parkinson's disease. Free Radic. Res. (2005) 39 1119 1125 10.1080/10715760500233113 16298737

상세보기

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증