A novel red colorants producing bacteria, Zooshikella rubidus S1-1 was selected to develop new red colorants for fabrics. The red colorants were purified from the novel bacteria and characterized. To determine biological functions and bio-safeties of purified colorants, cell culture and animal trial...
A novel red colorants producing bacteria, Zooshikella rubidus S1-1 was selected to develop new red colorants for fabrics. The red colorants were purified from the novel bacteria and characterized. To determine biological functions and bio-safeties of purified colorants, cell culture and animal trials were carried out. At the same time, the optimum pH and temperature of dyeing conditions for multifiber fabric were determined by dyeing properties. It notified that silk fabric is the best choice for the red colorants dyeing from Zooshikella rubidus S1-1 by the result of washing fastness and mordants test. Thus, optimum dyeing conditions, the concentration of colorants, pH and temperature, were determined by using silk fabric. Here we summarized right from the characterization of the microbial red colorants to dyeing on silk fabric. 1. The red colorants from new bacteria species were partially purified by using ultrasonication and solvent extraction from microbial cell and identified cycloprodigiosin and prodigiosin as the main components and five prodigiosin analogues by Liquid Chromatography-Massspectroscopy /Massspectroscopy (LC-MS/MS). H-NMR and FT-IR, analysis showed that the colorants have functional chemical groups such as N-H, C=C, and O-H. It could be applied as colorants for dyeing fabrics. The maximum spectrophotometric absorbance of the microbial red colorants is 530 nm, and it is chemically stable at 30-70℃ according to DSC analysis with a suitable dyeing temperature. 2. Skin and eye irritation toxicological tests of the red colorants in its usual application (0.3 mg/site/ml) were performed and it revealed that red colorants are a mild- or non-irritant, thus we confirmed its safety for dyeing of fabrics. Moreover, it is not only having an excellent and strong antibacterial activities against Staphylococcus epidermidis, Staphylococcus aureus, Bacillus subtilis, Streptococcus pneumonia among 38 species of pathogenic bacteria, but also having anticancer activities against black cutaneous cancer (SKMEL-28, A375P). 3. The colourimetric analysis of dyed multifiber fabric revealed the maximum K/S value of 13 fabrics dyed with the red colorants from Zooshikella rubidus S1-1 absorbs at 540nm. Silk, nylon, diacetate cotton and wool show the best ability to take up the dye with this absorbance. In the Munsell System of Color Notation, the colorants showed 4RP-8RP which corresponded to red-purple. The dyeability of fabric is dependent on pH and temperature of dyeing condition. AI and Ti as a mordant were increased on the dye uptake of fabric, whereas mordanting effects on the various mordants of the fabrics were diversified. 4. Optimum dyeing conditions for silk fabric were determined. The optimum dyeing concentration, pH, temperature and time were 1 % (o.w.f), pH 4.0, 60℃ and 60 min, respectively, and the optimum concentration of AI and Ti as mordant were shown as 3 % (o.w.f) and 0.7 % (o.w.f). 5. Dyed silk fabric with the microbial red colorants under the optimum conditions were tested for the fastness about water, washing, rubbing, perspiration, and dry cleaning. Most of color fastness using Al, Ti as mordant were increased as 3-5 grades, whereas light fastness was decrease as 1 grade. This study shows that the red colorants from Zooshikella rubidus S1-1 can facilitate natural colorant for fabric dyeing and it can be applied to natural colorants resource with bio-safeties and biological functions such as antibacterial and anticancer activity. Finally, it suggested that textile technology of fabric dye may be synergistic possibilities with the aid or fusion of modern biotechnology.
A novel red colorants producing bacteria, Zooshikella rubidus S1-1 was selected to develop new red colorants for fabrics. The red colorants were purified from the novel bacteria and characterized. To determine biological functions and bio-safeties of purified colorants, cell culture and animal trials were carried out. At the same time, the optimum pH and temperature of dyeing conditions for multifiber fabric were determined by dyeing properties. It notified that silk fabric is the best choice for the red colorants dyeing from Zooshikella rubidus S1-1 by the result of washing fastness and mordants test. Thus, optimum dyeing conditions, the concentration of colorants, pH and temperature, were determined by using silk fabric. Here we summarized right from the characterization of the microbial red colorants to dyeing on silk fabric. 1. The red colorants from new bacteria species were partially purified by using ultrasonication and solvent extraction from microbial cell and identified cycloprodigiosin and prodigiosin as the main components and five prodigiosin analogues by Liquid Chromatography-Massspectroscopy /Massspectroscopy (LC-MS/MS). H-NMR and FT-IR, analysis showed that the colorants have functional chemical groups such as N-H, C=C, and O-H. It could be applied as colorants for dyeing fabrics. The maximum spectrophotometric absorbance of the microbial red colorants is 530 nm, and it is chemically stable at 30-70℃ according to DSC analysis with a suitable dyeing temperature. 2. Skin and eye irritation toxicological tests of the red colorants in its usual application (0.3 mg/site/ml) were performed and it revealed that red colorants are a mild- or non-irritant, thus we confirmed its safety for dyeing of fabrics. Moreover, it is not only having an excellent and strong antibacterial activities against Staphylococcus epidermidis, Staphylococcus aureus, Bacillus subtilis, Streptococcus pneumonia among 38 species of pathogenic bacteria, but also having anticancer activities against black cutaneous cancer (SKMEL-28, A375P). 3. The colourimetric analysis of dyed multifiber fabric revealed the maximum K/S value of 13 fabrics dyed with the red colorants from Zooshikella rubidus S1-1 absorbs at 540nm. Silk, nylon, diacetate cotton and wool show the best ability to take up the dye with this absorbance. In the Munsell System of Color Notation, the colorants showed 4RP-8RP which corresponded to red-purple. The dyeability of fabric is dependent on pH and temperature of dyeing condition. AI and Ti as a mordant were increased on the dye uptake of fabric, whereas mordanting effects on the various mordants of the fabrics were diversified. 4. Optimum dyeing conditions for silk fabric were determined. The optimum dyeing concentration, pH, temperature and time were 1 % (o.w.f), pH 4.0, 60℃ and 60 min, respectively, and the optimum concentration of AI and Ti as mordant were shown as 3 % (o.w.f) and 0.7 % (o.w.f). 5. Dyed silk fabric with the microbial red colorants under the optimum conditions were tested for the fastness about water, washing, rubbing, perspiration, and dry cleaning. Most of color fastness using Al, Ti as mordant were increased as 3-5 grades, whereas light fastness was decrease as 1 grade. This study shows that the red colorants from Zooshikella rubidus S1-1 can facilitate natural colorant for fabric dyeing and it can be applied to natural colorants resource with bio-safeties and biological functions such as antibacterial and anticancer activity. Finally, it suggested that textile technology of fabric dye may be synergistic possibilities with the aid or fusion of modern biotechnology.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.