This study was carried out to investigate the antimocrobial activities of organic extracts obtained from the fruit of Thuja orientalis L. The fruits native in korea were collected and extracted by 80% ethanol, and the extract was sequentially fractionated with n-hexane, chloroform, ethylacetate, but...
This study was carried out to investigate the antimocrobial activities of organic extracts obtained from the fruit of Thuja orientalis L. The fruits native in korea were collected and extracted by 80% ethanol, and the extract was sequentially fractionated with n-hexane, chloroform, ethylacetate, butanol and aqueous layer. The fraction yields of n-hexane, chloroform, ethylacetate, butanol and water of ethanol extract were 10.15%, 10.05%, 1or5%, r5.35% and 5%.55%, respectively. n-Hexane-soluble fraction showed the highest antibacterial activity against gram positive bacteria, while the chloroform, ethylacetate, butanol and aqueous fractions did not show any antibacterial actvity. Minimum inhibitory concentration(MICs) on Staphyloylccus aureus, Bacillus subtilis and Sateptoylccus pneumoniae, n-hexane-soluble fraction were 100 ㎍, 500 ㎍ and 50 ㎍/disc, respectively. The antibacterial activity was not destroyed by heating at 80, 100, 120℃ for 30 min and was not affected by pH. In the inhibition test of the Staphylococcus aureus, Bacillus subtilis and Sateptococcus pneumoniae, n-hexane-soluble fraction showed potent growth inhibition at concentration of 0.1, 0.5 ㎍/mL for 12～24 hours. However, n-hexane-soluble fraction did not show any mutagenic activity.
This study was carried out to investigate the antimocrobial activities of organic extracts obtained from the fruit of Thuja orientalis L. The fruits native in korea were collected and extracted by 80% ethanol, and the extract was sequentially fractionated with n-hexane, chloroform, ethylacetate, butanol and aqueous layer. The fraction yields of n-hexane, chloroform, ethylacetate, butanol and water of ethanol extract were 10.15%, 10.05%, 1or5%, r5.35% and 5%.55%, respectively. n-Hexane-soluble fraction showed the highest antibacterial activity against gram positive bacteria, while the chloroform, ethylacetate, butanol and aqueous fractions did not show any antibacterial actvity. Minimum inhibitory concentration(MICs) on Staphyloylccus aureus, Bacillus subtilis and Sateptoylccus pneumoniae, n-hexane-soluble fraction were 100 ㎍, 500 ㎍ and 50 ㎍/disc, respectively. The antibacterial activity was not destroyed by heating at 80, 100, 120℃ for 30 min and was not affected by pH. In the inhibition test of the Staphylococcus aureus, Bacillus subtilis and Sateptococcus pneumoniae, n-hexane-soluble fraction showed potent growth inhibition at concentration of 0.1, 0.5 ㎍/mL for 12～24 hours. However, n-hexane-soluble fraction did not show any mutagenic activity.
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