Objectives : This study was performed to investigate the anti-fibrogenic effect of Angelica Sinens Radix on cultured rat hepatic stellate cells.
Materials and Methods : Hepatic stellate cells(HSC-T6) were treated with various concentrations of Angelica Sinens Radix extract for both 24 and 4...
Objectives : This study was performed to investigate the anti-fibrogenic effect of Angelica Sinens Radix on cultured rat hepatic stellate cells.
Materials and Methods : Hepatic stellate cells(HSC-T6) were treated with various concentrations of Angelica Sinens Radix extract for both 24 and 48hours. The extraction was done either with distilled water or 80% EtOH. After the treatment, cell viability, proliferation, procollagen levels and the mRNA of the ASMA, TIMP1, TIMP2, procollagen Type 1a2, Cytokine IL-6 were measured by using MTT assay, BrdU assay, RT-PCR, and procollagen Type I C-peptide EIA Kit, IL-6 ELISA assay. Results : The viability of the hepatic stellate cells in the extraction made with distilled water was significantly increased, but there were no significant changes in 80% EtOH extraction. The proliferation of the hepatic stellate cells in the extraction made with distilled water was decreased only in the 24 hours group, while there were significant decreases both in the 24 and 48 hours group in 80% EtOH extraction ; Especially it was more decreased in 48 hours group. The mRNA expressions of the ASMA, TIMP2 and collagen1a2 decreased in a concentration dependent manner in the 48 hours group. Procollagen Production decreased in a concentration dependent manner in the both 24, 48 hours group. The production of the Cytokine increased in a concentration dependent manner in the both 24, 48 hours group.
Conclusion : These results suggest that Angelica Sinens Radix is beneficial in the treatment of cirrhotic patients as well as for the patients with chronic hepatitis.
Objectives : This study was performed to investigate the anti-fibrogenic effect of Angelica Sinens Radix on cultured rat hepatic stellate cells.
Materials and Methods : Hepatic stellate cells(HSC-T6) were treated with various concentrations of Angelica Sinens Radix extract for both 24 and 48hours. The extraction was done either with distilled water or 80% EtOH. After the treatment, cell viability, proliferation, procollagen levels and the mRNA of the ASMA, TIMP1, TIMP2, procollagen Type 1a2, Cytokine IL-6 were measured by using MTT assay, BrdU assay, RT-PCR, and procollagen Type I C-peptide EIA Kit, IL-6 ELISA assay. Results : The viability of the hepatic stellate cells in the extraction made with distilled water was significantly increased, but there were no significant changes in 80% EtOH extraction. The proliferation of the hepatic stellate cells in the extraction made with distilled water was decreased only in the 24 hours group, while there were significant decreases both in the 24 and 48 hours group in 80% EtOH extraction ; Especially it was more decreased in 48 hours group. The mRNA expressions of the ASMA, TIMP2 and collagen1a2 decreased in a concentration dependent manner in the 48 hours group. Procollagen Production decreased in a concentration dependent manner in the both 24, 48 hours group. The production of the Cytokine increased in a concentration dependent manner in the both 24, 48 hours group.
Conclusion : These results suggest that Angelica Sinens Radix is beneficial in the treatment of cirrhotic patients as well as for the patients with chronic hepatitis.
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