This study was investigated antioxidant effects and physiological activities of Pumpkin (Cucurbita moschata Duch.) extract from various parts such as leaf, flesh, skin, seed. Dried different parts of C. moschata were boiled in ethanol for three hours, evaporated and lyophilized with a freeze-dryer. ...
This study was investigated antioxidant effects and physiological activities of Pumpkin (Cucurbita moschata Duch.) extract from various parts such as leaf, flesh, skin, seed. Dried different parts of C. moschata were boiled in ethanol for three hours, evaporated and lyophilized with a freeze-dryer. Extraction yield and general component of each ethanolic extract from various parts of C. mo-schata were investigated. The ethanolic extracts of C. moschata were tested its antioxidant activities using 1,1-duogebyk-2-picrylhydrazyl free radical-scavenging activity (DPPH) assay, ABTS assay assay and ferric-reducing anti-oxidant power (FRAP) assay and measuring total polyphenol content, flavonoid content. And ethanolic extracts of C. moschata were tested in physiological ac-tivities such as anti-tyrosinase, anti-glycation and anti-alpha-glucosidase.
Extraction yield of flesh part was the highest. The moisture contents of leaf, flesh, skin and seed were 88.25%, 93.55%, 94.16%, 51.85% respectively. The contents of protein, lipid, ash of seed part were higher than other parts. Total polyphenol contents were the highest in leaf (29.62 ± 0.88 mg GAE/g DM), fol-lowed by skin (7.08 ± 0.84 mg GAE/g DM), flesh (12.08 ± 0.54 mg GAE/g DM), seed (1.52 ± 0.53 mg GAE/g DM). The flavonoid contents followed the same results. The leaf extract of C. moschata had stronger DPPH SC50 (the es-timated amounts of samples that could reduce initial absorbance by 50%) than any other part extract (leaf; 1.27 mg DM/mL, flesh; 3.85 mg DM/mL, skin; 2.83mg DM/mL and seed; 7.33 mg DM/mL).
Based on the results of the ABTS radical inhibiting assay, the leaf showed a value of 2.28 mg DM/mL. Reducing power was determined by FRAP method. FeSO4•7H2O was used as a spectrometric standard. The leaf extract of Cucur-bita moschata Duch. was found to be the highest reducing power. Meanwhile, 0.1 mg/mL of all extract showed higher degree of anti-tyrosinase effects than kojic acid at the same concentration (leaf; 49.58%, flesh; 40.20%, skin; 41.30%, seed; 36.79% and kojic acid; 20.70%). The glycation inhibitory activity was reduced compared to control followed by leaf (62.85%), skin (50.14%), flesh (13.72%) and seed (11.18%). The anti-alpha glucosidase ability of seed extract was the highest with a value of 63.58% at a concentration of 1 mg/mL.
In summary, the leaf extract of C. moschata had more effect of antioxidant and physiological activities than other parts, however, different parts showed some effective activities such as alpha-glucisedase inhibition. These results may suggest that further in vivo studies of C. moschata are remained to investigate.
This study was investigated antioxidant effects and physiological activities of Pumpkin (Cucurbita moschata Duch.) extract from various parts such as leaf, flesh, skin, seed. Dried different parts of C. moschata were boiled in ethanol for three hours, evaporated and lyophilized with a freeze-dryer. Extraction yield and general component of each ethanolic extract from various parts of C. mo-schata were investigated. The ethanolic extracts of C. moschata were tested its antioxidant activities using 1,1-duogebyk-2-picrylhydrazyl free radical-scavenging activity (DPPH) assay, ABTS assay assay and ferric-reducing anti-oxidant power (FRAP) assay and measuring total polyphenol content, flavonoid content. And ethanolic extracts of C. moschata were tested in physiological ac-tivities such as anti-tyrosinase, anti-glycation and anti-alpha-glucosidase.
Extraction yield of flesh part was the highest. The moisture contents of leaf, flesh, skin and seed were 88.25%, 93.55%, 94.16%, 51.85% respectively. The contents of protein, lipid, ash of seed part were higher than other parts. Total polyphenol contents were the highest in leaf (29.62 ± 0.88 mg GAE/g DM), fol-lowed by skin (7.08 ± 0.84 mg GAE/g DM), flesh (12.08 ± 0.54 mg GAE/g DM), seed (1.52 ± 0.53 mg GAE/g DM). The flavonoid contents followed the same results. The leaf extract of C. moschata had stronger DPPH SC50 (the es-timated amounts of samples that could reduce initial absorbance by 50%) than any other part extract (leaf; 1.27 mg DM/mL, flesh; 3.85 mg DM/mL, skin; 2.83mg DM/mL and seed; 7.33 mg DM/mL).
Based on the results of the ABTS radical inhibiting assay, the leaf showed a value of 2.28 mg DM/mL. Reducing power was determined by FRAP method. FeSO4•7H2O was used as a spectrometric standard. The leaf extract of Cucur-bita moschata Duch. was found to be the highest reducing power. Meanwhile, 0.1 mg/mL of all extract showed higher degree of anti-tyrosinase effects than kojic acid at the same concentration (leaf; 49.58%, flesh; 40.20%, skin; 41.30%, seed; 36.79% and kojic acid; 20.70%). The glycation inhibitory activity was reduced compared to control followed by leaf (62.85%), skin (50.14%), flesh (13.72%) and seed (11.18%). The anti-alpha glucosidase ability of seed extract was the highest with a value of 63.58% at a concentration of 1 mg/mL.
In summary, the leaf extract of C. moschata had more effect of antioxidant and physiological activities than other parts, however, different parts showed some effective activities such as alpha-glucisedase inhibition. These results may suggest that further in vivo studies of C. moschata are remained to investigate.
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