모든 GM 작물에는 삽입된 유전자를 발현하기 위한 promoter와 terminator 및 selectable marker가 포함되어 있으며, 이들 구성요소들은 미승인된 유전자 재조합 작물의 동정에 유용하게 사용된다. 그래서 위 논문은 세 종류의 promoters (35S promoter, NOS promoter, and ...
모든 GM 작물에는 삽입된 유전자를 발현하기 위한 promoter와 terminator 및 selectable marker가 포함되어 있으며, 이들 구성요소들은 미승인된 유전자 재조합 작물의 동정에 유용하게 사용된다. 그래서 위 논문은 세 종류의 promoters (35S promoter, NOS promoter, and FMV promoter), 두 종류의 terminator (35S terminator and NOS terminator), 세 종류의 selectable markers (NPTII, AAD, and GUS)에 특이적인 primer 제조 및 이들을 이용하여 multiplex PCR 방법을 개발하였다. Primer의 특이성을 확인하기 위하여 유전자 재조합 콩 (Roundup Ready Soybean and A2704-12), 옥수수 (GA21, LY038, MON810, MON863, MON89034, DAS59122, NK603 and T25), 파파야(55-1)가 사용되었다. Multiplex PCR 산물은 일반적으로 사용되는 agarose gel 전기영동법 외에 Agilent 2100 Bioanalyzer를 사용하여 분석하였다.
모든 GM 작물에는 삽입된 유전자를 발현하기 위한 promoter와 terminator 및 selectable marker가 포함되어 있으며, 이들 구성요소들은 미승인된 유전자 재조합 작물의 동정에 유용하게 사용된다. 그래서 위 논문은 세 종류의 promoters (35S promoter, NOS promoter, and FMV promoter), 두 종류의 terminator (35S terminator and NOS terminator), 세 종류의 selectable markers (NPTII, AAD, and GUS)에 특이적인 primer 제조 및 이들을 이용하여 multiplex PCR 방법을 개발하였다. Primer의 특이성을 확인하기 위하여 유전자 재조합 콩 (Roundup Ready Soybean and A2704-12), 옥수수 (GA21, LY038, MON810, MON863, MON89034, DAS59122, NK603 and T25), 파파야(55-1)가 사용되었다. Multiplex PCR 산물은 일반적으로 사용되는 agarose gel 전기영동법 외에 Agilent 2100 Bioanalyzer를 사용하여 분석하였다.
All genetically modified (GM) crops contain promoter and terminator for gene expression and selectable marker for genetically modified cell selection, thereby detecting these elements are useful for identification of unapproved genetically modified crops. Therefore, the aim of this study is developm...
All genetically modified (GM) crops contain promoter and terminator for gene expression and selectable marker for genetically modified cell selection, thereby detecting these elements are useful for identification of unapproved genetically modified crops. Therefore, the aim of this study is development of multiplex PCR (polymerase chain reaction) using primers for three promoters (35S promoter, NOS promoter, and FMV promoter), two terminators (35S terminator and NOS terminator) and three selectable markers (NPTII, AAD, and GUS). To test specificity, genetically modified soybean (Roundup Ready Soybean and A2704-12), maize (GA21, LY038, MON810, MON863, MON89034, DAS59122, NK603 and T25) and papaya (55-1) were used. Multiplex PCR products were analyzed by using Agilent 2100 Bioanalyzer that is modification of capillary electrophoresis as well as conventional agarose gel electrophoresis. The electrophoresis results showed that results of Agilent 2100 Bioanalyzer were more accurate and the data was easily analyzed compared with agarose gel electrophoresis results.
All genetically modified (GM) crops contain promoter and terminator for gene expression and selectable marker for genetically modified cell selection, thereby detecting these elements are useful for identification of unapproved genetically modified crops. Therefore, the aim of this study is development of multiplex PCR (polymerase chain reaction) using primers for three promoters (35S promoter, NOS promoter, and FMV promoter), two terminators (35S terminator and NOS terminator) and three selectable markers (NPTII, AAD, and GUS). To test specificity, genetically modified soybean (Roundup Ready Soybean and A2704-12), maize (GA21, LY038, MON810, MON863, MON89034, DAS59122, NK603 and T25) and papaya (55-1) were used. Multiplex PCR products were analyzed by using Agilent 2100 Bioanalyzer that is modification of capillary electrophoresis as well as conventional agarose gel electrophoresis. The electrophoresis results showed that results of Agilent 2100 Bioanalyzer were more accurate and the data was easily analyzed compared with agarose gel electrophoresis results.
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