To establish the effective in vitro propagation system for Lycoris sanguinea Maxim. var. koreana (Nakai) Koyama, various organs including seeds, anther, filament, ovary, petal, pedicel and bulbscales were cultured on the MS medium with 2,4-D, NAA, IBA and BA. Among those, seeds and bulbscales were t...
To establish the effective in vitro propagation system for Lycoris sanguinea Maxim. var. koreana (Nakai) Koyama, various organs including seeds, anther, filament, ovary, petal, pedicel and bulbscales were cultured on the MS medium with 2,4-D, NAA, IBA and BA. Among those, seeds and bulbscales were the most suitable for in vitro multiplication. The seeds collected 30 days or 50 days after anthesis showed the germination rate of 92 and 98%, respectively, on the MS medium with no plant hormone. The formation of seed-derived callus and subsequent regeneration of multiple bulblets were best on the MS medium with 0.1 mg·L-1 2,4-D and 1.0 mg·L-1 BA. The explants such as ovaries, pedicels and petals in the floral bud produced just a small quantity of callus, as explants were collected at intervals from 10 days before anthesis to the day of flower. However, bulblet regeneration from the callus was not observed. For the bulbscale culture, the bulbs treated at 5℃ for 60 days were dissected into three parts according to bulb height(upper, middle and bottom), and allowed for a culture on the MS medium with 0.1 mg·L-1 2,4-D plus 1.0 mg·L-1 BA, 0.5 mg·L-1 NAA plus 1.0 mg·L-1 BA, and 1.0 mg·L-1 IBA plus 1.0 mg·L-1 BA. Regardless of plant hormone treatments, the bottom part of the bulb including the disk proved to be most effective in forming the callus and bulblets. The MS medium with 3.0 mg·L-1 IBA plus 3.0 mg·L-1 BA proved to be best effective in inducing bublets from the callus, where about 7～8 bulblets per explant were formed. Bulblet enlargement was best when 6% sucrose was added to the MS medium with 3.0 mg·L-1 IBA plus 3.0 mg·L-1 BA, compared to 3% or 9% sucrose.
To establish the effective in vitro propagation system for Lycoris sanguinea Maxim. var. koreana (Nakai) Koyama, various organs including seeds, anther, filament, ovary, petal, pedicel and bulbscales were cultured on the MS medium with 2,4-D, NAA, IBA and BA. Among those, seeds and bulbscales were the most suitable for in vitro multiplication. The seeds collected 30 days or 50 days after anthesis showed the germination rate of 92 and 98%, respectively, on the MS medium with no plant hormone. The formation of seed-derived callus and subsequent regeneration of multiple bulblets were best on the MS medium with 0.1 mg·L-1 2,4-D and 1.0 mg·L-1 BA. The explants such as ovaries, pedicels and petals in the floral bud produced just a small quantity of callus, as explants were collected at intervals from 10 days before anthesis to the day of flower. However, bulblet regeneration from the callus was not observed. For the bulbscale culture, the bulbs treated at 5℃ for 60 days were dissected into three parts according to bulb height(upper, middle and bottom), and allowed for a culture on the MS medium with 0.1 mg·L-1 2,4-D plus 1.0 mg·L-1 BA, 0.5 mg·L-1 NAA plus 1.0 mg·L-1 BA, and 1.0 mg·L-1 IBA plus 1.0 mg·L-1 BA. Regardless of plant hormone treatments, the bottom part of the bulb including the disk proved to be most effective in forming the callus and bulblets. The MS medium with 3.0 mg·L-1 IBA plus 3.0 mg·L-1 BA proved to be best effective in inducing bublets from the callus, where about 7～8 bulblets per explant were formed. Bulblet enlargement was best when 6% sucrose was added to the MS medium with 3.0 mg·L-1 IBA plus 3.0 mg·L-1 BA, compared to 3% or 9% sucrose.
Keyword
#explants 2,4-D NAA IBA BA
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