Abstract ▼ AI-Helper

This study was aimed to determine the effective conditions for bulblet regeneration from scale tissue culture and use as basic data for the horticultural use and new cultivar breeding of Lycoris koreana by investigating the types, combinations, and concentrations of plant growth regulators and the o...

This study was aimed to determine the effective conditions for bulblet regeneration from scale tissue culture and use as basic data for the horticultural use and new cultivar breeding of Lycoris koreana by investigating the types, combinations, and concentrations of plant growth regulators and the organic contents of scale at different scale digging time in Lycoris koreana. Bulblet regeneration was observed from the bottom inside of bulb scales at 6~8 weeks after culture began. For bulblet regeneration, 1.0 mg·L-1 NAA + 1.0mg·L-1 BA and 0.5mg·L-1 NAA +0.5mg·L-1 TDZ were more effective. Bulblet macropagation was observed from subculture on the MS medium with 1.0 mg·L-1 NAA +1.0mg·L-1 BA. In the analysis of organic contents in bulb scales at different growth stages, total sugar content, starch content, amino acids and protein contents were the highest in the basal part at flowering, the middle part of bulb scales at leaf emergence time, and the basal part of bulb scales before flowering, respectively. The results of this study are expected to use as important basic data for native flower development and new cultivar breeding by determining the bulblet regeneration condition of Lycoris koreana, and help to quicken the macropropagation and new cultivar breeding period.

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