Purslane (Protulaca oleracea) is a kind of annual plant which belongs to Portulacaceae and commonly called "Shebirum" in Korea. It can be frequently seen in such places as vegetable gardens and empty spaces as well as any roadside. In Korea, people used to eat the parts of the plant after blanching ...
Purslane (Protulaca oleracea) is a kind of annual plant which belongs to Portulacaceae and commonly called "Shebirum" in Korea. It can be frequently seen in such places as vegetable gardens and empty spaces as well as any roadside. In Korea, people used to eat the parts of the plant after blanching them. In the Wsetern style, it can be mainly used with lettuce for salads. Also, it can be dried and used as a material for various dishes as well as tea or soup. Especially, purslane shows relatively higher amounts biological components and nutrients of including the ω-3 fatty acid and γ-linolenic acid than those of other plants, and has been reported to suppress cancer or be effective muscle relaxation and antimicrobial activity. This study was performed to the nutritional properties and biological activities of hot water and 80% ethanol extracts from purslane. Also, the biological activities such as antioxidant, antimicrobial and anticancer were measured from fractions from 80% methanol extract of purslane. In vivo experiment, in order to verify the biological effect of purslane for hyperlipidemia, this study focuses on the effects of lyophilized purslane powder on the change of the lipid components in serum and liver tissues of hypercholesterolemic rat. 1. The proximate composition was analyzed of purslane. Based on the dry matter, the moisture content was 5.14%, while the contnet of crude fiber was 31.35%, being the greatest. Total mineral contents was 6025.80 mg/100 g, potassium was the highest by 3846.99 mg/100 g. 2. We investigate to the antioxidant activity of purslane for biological properties. Yields of the water and 80% ethanol extracts was 14.84% and 24.93%, respectively. The contents of phenolic and flavonoids in ethanol extract were 58.16 mg/g and 20.08 mg/g, their contents were ignificantly higher in ethanol extracts than water extracts, respectively. Activities of antioxidant and nitrite scavenging significantly elevated in a dose-dependent manner, and those activities were higher in ethanol extract than the water extract. Activity of α-Glucosidase inhibition was significantly higher in ethanol extract than the water extract, also. 3. After dried purslane was extracted with 80% methanol, and then it was further fractionated into five subfractions by using solvent partition method, affording hexane, chloroform, ethyl acetate, butanol and water. The content of total polyphenol from purslane extract showed the highest value in ethyl acetate fraction (303.61 mg/g). Antioxidant activities of different solvent fractions by DPPH, ABTS radical scavenging and FRAP assay were increased by higher in ethyl acetate, water and chloroform fractions. Whereas, Fe2+ chelating activity was higher in 80%methanol extract, butanol and hexane fractions. 4. The solvent fractions of purslane were tested for their antimicrobial activity against several food spoilage microorganism including Escherichia coli and Staphylococcus aureus. It was analyzed in 50, 100, 200 and 300 mg/mL concentrations. The antimicrobial activities were increased with increasing concentration of the solvent fractions of purslane. Among the various solvent fractions, water fraction was shown the strongest antimicrobial activities. Besides ethyl actate fraction and 80% methanol extract showed significantly higher activity. It is believed that solvent fractions of the purslane would be useful as natural antimicrobial substances. 5. The growth inhibition activity in different solvent fractions from 80% methanol extract of purslane on the two human cancer cell lines such as human breast adenocarcinoma cell line MCF-7 and human stomach ademocarcinoma cell line AGS was measured. Growth inhibition activity of solvent fractions was assayed in 10, 30, 50, 100 and 200 μg/mL concentrations. All fractions of purslane increased significantly in dependent on the concentration. Especially, the rates of growth inhibition in hexane and chloroform fractions of purslane were more than 80% inhibition activity on MCF-7 and 70% inhibition activity on AGS cell, at the concentration of 200 μg/mL, and which were higher than another fractions. 6. It was tested to compare the effect of purslane powder on lipid improvements in serum and liver tissue of 1% cholesterol-induced hypercholesterolemic rats. The urslane powder was supplemented by 5% and 10% with a high cholesterol diet. The concentration of total lipids, total cholesterol and LDL-cholesterol in serum were significantly decreased in 10% than 5% fed group of purslane powder. GOT, GPT and ALP activities in serum were significantly decreased in groups fed from purslane powder compared with control group. Levels of total lipids, total cholesterol and triglyceride in liver tissue tended to decrease in groups fed from purslane powder compared with control group. Lipid peroxidation level in serum and liver tissue was significantly decreased in group fed from purslane powder compared with control group. Fecal lipid concentration in feces was significantly higher in group fed from purslane powder, moreover fecal total cholesterol and triglyceride levels were increased in purslane powder 10% fed groups compared with control group.
Purslane (Protulaca oleracea) is a kind of annual plant which belongs to Portulacaceae and commonly called "Shebirum" in Korea. It can be frequently seen in such places as vegetable gardens and empty spaces as well as any roadside. In Korea, people used to eat the parts of the plant after blanching them. In the Wsetern style, it can be mainly used with lettuce for salads. Also, it can be dried and used as a material for various dishes as well as tea or soup. Especially, purslane shows relatively higher amounts biological components and nutrients of including the ω-3 fatty acid and γ-linolenic acid than those of other plants, and has been reported to suppress cancer or be effective muscle relaxation and antimicrobial activity. This study was performed to the nutritional properties and biological activities of hot water and 80% ethanol extracts from purslane. Also, the biological activities such as antioxidant, antimicrobial and anticancer were measured from fractions from 80% methanol extract of purslane. In vivo experiment, in order to verify the biological effect of purslane for hyperlipidemia, this study focuses on the effects of lyophilized purslane powder on the change of the lipid components in serum and liver tissues of hypercholesterolemic rat. 1. The proximate composition was analyzed of purslane. Based on the dry matter, the moisture content was 5.14%, while the contnet of crude fiber was 31.35%, being the greatest. Total mineral contents was 6025.80 mg/100 g, potassium was the highest by 3846.99 mg/100 g. 2. We investigate to the antioxidant activity of purslane for biological properties. Yields of the water and 80% ethanol extracts was 14.84% and 24.93%, respectively. The contents of phenolic and flavonoids in ethanol extract were 58.16 mg/g and 20.08 mg/g, their contents were ignificantly higher in ethanol extracts than water extracts, respectively. Activities of antioxidant and nitrite scavenging significantly elevated in a dose-dependent manner, and those activities were higher in ethanol extract than the water extract. Activity of α-Glucosidase inhibition was significantly higher in ethanol extract than the water extract, also. 3. After dried purslane was extracted with 80% methanol, and then it was further fractionated into five subfractions by using solvent partition method, affording hexane, chloroform, ethyl acetate, butanol and water. The content of total polyphenol from purslane extract showed the highest value in ethyl acetate fraction (303.61 mg/g). Antioxidant activities of different solvent fractions by DPPH, ABTS radical scavenging and FRAP assay were increased by higher in ethyl acetate, water and chloroform fractions. Whereas, Fe2+ chelating activity was higher in 80%methanol extract, butanol and hexane fractions. 4. The solvent fractions of purslane were tested for their antimicrobial activity against several food spoilage microorganism including Escherichia coli and Staphylococcus aureus. It was analyzed in 50, 100, 200 and 300 mg/mL concentrations. The antimicrobial activities were increased with increasing concentration of the solvent fractions of purslane. Among the various solvent fractions, water fraction was shown the strongest antimicrobial activities. Besides ethyl actate fraction and 80% methanol extract showed significantly higher activity. It is believed that solvent fractions of the purslane would be useful as natural antimicrobial substances. 5. The growth inhibition activity in different solvent fractions from 80% methanol extract of purslane on the two human cancer cell lines such as human breast adenocarcinoma cell line MCF-7 and human stomach ademocarcinoma cell line AGS was measured. Growth inhibition activity of solvent fractions was assayed in 10, 30, 50, 100 and 200 μg/mL concentrations. All fractions of purslane increased significantly in dependent on the concentration. Especially, the rates of growth inhibition in hexane and chloroform fractions of purslane were more than 80% inhibition activity on MCF-7 and 70% inhibition activity on AGS cell, at the concentration of 200 μg/mL, and which were higher than another fractions. 6. It was tested to compare the effect of purslane powder on lipid improvements in serum and liver tissue of 1% cholesterol-induced hypercholesterolemic rats. The urslane powder was supplemented by 5% and 10% with a high cholesterol diet. The concentration of total lipids, total cholesterol and LDL-cholesterol in serum were significantly decreased in 10% than 5% fed group of purslane powder. GOT, GPT and ALP activities in serum were significantly decreased in groups fed from purslane powder compared with control group. Levels of total lipids, total cholesterol and triglyceride in liver tissue tended to decrease in groups fed from purslane powder compared with control group. Lipid peroxidation level in serum and liver tissue was significantly decreased in group fed from purslane powder compared with control group. Fecal lipid concentration in feces was significantly higher in group fed from purslane powder, moreover fecal total cholesterol and triglyceride levels were increased in purslane powder 10% fed groups compared with control group.
주제어
#쇠비름 항산화 고콜레스테롤 혈증 항균 항암효과
※ AI-Helper는 부적절한 답변을 할 수 있습니다.