강원도 지역에서 자생하는 소나무(Pinus densiflora)의 솔잎(Pini Folium)을 이용하여 항산화, 항균 효능과 NHDF에 대한 세포독성 억제효능을 알아보고 COL1A1의 mRNA 발현과 pro-collagen 1 생성을 측정하였다. Pini Folium의 80% ...
강원도 지역에서 자생하는 소나무(Pinus densiflora)의 솔잎(Pini Folium)을 이용하여 항산화, 항균 효능과 NHDF에 대한 세포독성 억제효능을 알아보고 COL1A1의 mRNA 발현과 pro-collagen 1 생성을 측정하였다. Pini Folium의 80% ethanol 추출물을 PFE, 열수추출물을 PFW라고 명명하였다. DPPH radical scavenging activity를 비교하였고, Pini Folium추출물을 투여한 E. coli, S. aureus, P. aeruginosa, C. albicans, P. acnes에 대해 clear zone을 측정하였다. NHDF에 대한 MTT assay, TNF-α, H2O2의 세포독성 억제효능을 측정하였고, qRT-PCR을 이용하여 COL1AI 발현량과 Western blot을 통해 pro-collagen 1의 생성을 측정하였다. 실험 결과, Pini Folium 추출물의 DPPH radical scavenging activity는 PFE는 유의성이 없었고, PFW는 유의한 결과를 나타내었다. 항균효능 측정에서 PFE는 E. coli, S. aureus, P. acnes에 대한 항균효능을 나타내었고, PFW는 S. aureus, P. acnes에서만 항균효능이 나타났다. MTT assay 는 PFE, PFW 모두 진피세포에 대한 독성은 없었다. TNF-α의 세포사멸 억제 효능에서 PFE는 10 μg/mL의 농도에서 유의한 결과를 나타내었고, PFW는 유의성이 없었다. H2O2의 세포사멸 억제 효능에서 PFE, PFW 모두 유의한 결과를 확인할 수 있었다. COL1A1의 측정에서 PFE는 유의성이 없었고, PFW는 유의한 결과를 나타내었다. pro-collagen 1 생성에서는 PFE, PFW 모두 생성되는 것을 관찰하였다. 결론적으로 PFE와 PFW는 NHDF에 대한 COL1A1의 mRNA 발현과 pro-collagen 1 의 생성으로 Pini Folium추출물이 피부미용에 실용적으로 응용될 수 있고 피부노화에 있어 항노화 가능성을 제시한 결과라 사료된다.
강원도 지역에서 자생하는 소나무(Pinus densiflora)의 솔잎(Pini Folium)을 이용하여 항산화, 항균 효능과 NHDF에 대한 세포독성 억제효능을 알아보고 COL1A1의 mRNA 발현과 pro-collagen 1 생성을 측정하였다. Pini Folium의 80% ethanol 추출물을 PFE, 열수추출물을 PFW라고 명명하였다. DPPH radical scavenging activity를 비교하였고, Pini Folium추출물을 투여한 E. coli, S. aureus, P. aeruginosa, C. albicans, P. acnes에 대해 clear zone을 측정하였다. NHDF에 대한 MTT assay, TNF-α, H2O2의 세포독성 억제효능을 측정하였고, qRT-PCR을 이용하여 COL1AI 발현량과 Western blot을 통해 pro-collagen 1의 생성을 측정하였다. 실험 결과, Pini Folium 추출물의 DPPH radical scavenging activity는 PFE는 유의성이 없었고, PFW는 유의한 결과를 나타내었다. 항균효능 측정에서 PFE는 E. coli, S. aureus, P. acnes에 대한 항균효능을 나타내었고, PFW는 S. aureus, P. acnes에서만 항균효능이 나타났다. MTT assay 는 PFE, PFW 모두 진피세포에 대한 독성은 없었다. TNF-α의 세포사멸 억제 효능에서 PFE는 10 μg/mL의 농도에서 유의한 결과를 나타내었고, PFW는 유의성이 없었다. H2O2의 세포사멸 억제 효능에서 PFE, PFW 모두 유의한 결과를 확인할 수 있었다. COL1A1의 측정에서 PFE는 유의성이 없었고, PFW는 유의한 결과를 나타내었다. pro-collagen 1 생성에서는 PFE, PFW 모두 생성되는 것을 관찰하였다. 결론적으로 PFE와 PFW는 NHDF에 대한 COL1A1의 mRNA 발현과 pro-collagen 1 의 생성으로 Pini Folium추출물이 피부미용에 실용적으로 응용될 수 있고 피부노화에 있어 항노화 가능성을 제시한 결과라 사료된다.
By using Pini Folium of Pinus densiflora Seib. et Zucc. that naturally grow in Gangwon-do area, anti-oxidant, antibacterial effect, and cytotoxicity resistance effect against NHDF were examined and a study on the mRNA expression of COL1A1 and generation effect of pro-collagen 1 was conducted. After ...
By using Pini Folium of Pinus densiflora Seib. et Zucc. that naturally grow in Gangwon-do area, anti-oxidant, antibacterial effect, and cytotoxicity resistance effect against NHDF were examined and a study on the mRNA expression of COL1A1 and generation effect of pro-collagen 1 was conducted. After extracting Pini Folium with 80% ethanol and hot water respectively and they were referred to as PEF and PFW. DPPH radical scavenging activity was compared, clear zone on E. coli, S. aureus, P. aeruginosa, C. albicans, P. acnes treated with Pini Folium extract was measured. The cytotoxicity resistance effect of pine needles was examined by conducting MTT assay, TNF-α, and H2O2 test regarding NHDF. The amount of COL1A1 expression using qRT-PCR and the generation of pro-collagen 1 using Western blot were examined. As a result of the experiment, DPPH radical scavenging activity of Pini Folium extract didn't have non-significance in PFE, it had a significance in PFW. In regards to the paper disc method measuring antibacterial effect, PFE displayed antibacterial effect against E. coli, S. aureus, P. acnes, PFW displayed antibacterial effect against S. aureus, P. acnes.. In MTT assay, PFE, PFW had no cytotoxicity against the hypodermal cell. As a result of cell death resistance effect on TNF-α, PFE displayed a significant result in the concentration of 10 μg/mL and PFW displayed non signifiant result. In the effects on cell death resistance of H2O2, PFE and PFW could be confirmed to have significant results. In regards to the measurement of mRNA expression of COL1A1, PFE displayed non-significant difference, PFW displayed significant difference. In regards to the generation of pro-collagen 1, the protein was generated for both PFE, PFW. In conclusion, PFE and PFW of Pini Folium extract can be applied to skin beauty practically and considered as the results to suggest the anti-aging possibility in aging skin in regards to mRNA expression of COL1A1 against NHDF and generation of pro-collagen 1.
By using Pini Folium of Pinus densiflora Seib. et Zucc. that naturally grow in Gangwon-do area, anti-oxidant, antibacterial effect, and cytotoxicity resistance effect against NHDF were examined and a study on the mRNA expression of COL1A1 and generation effect of pro-collagen 1 was conducted. After extracting Pini Folium with 80% ethanol and hot water respectively and they were referred to as PEF and PFW. DPPH radical scavenging activity was compared, clear zone on E. coli, S. aureus, P. aeruginosa, C. albicans, P. acnes treated with Pini Folium extract was measured. The cytotoxicity resistance effect of pine needles was examined by conducting MTT assay, TNF-α, and H2O2 test regarding NHDF. The amount of COL1A1 expression using qRT-PCR and the generation of pro-collagen 1 using Western blot were examined. As a result of the experiment, DPPH radical scavenging activity of Pini Folium extract didn't have non-significance in PFE, it had a significance in PFW. In regards to the paper disc method measuring antibacterial effect, PFE displayed antibacterial effect against E. coli, S. aureus, P. acnes, PFW displayed antibacterial effect against S. aureus, P. acnes.. In MTT assay, PFE, PFW had no cytotoxicity against the hypodermal cell. As a result of cell death resistance effect on TNF-α, PFE displayed a significant result in the concentration of 10 μg/mL and PFW displayed non signifiant result. In the effects on cell death resistance of H2O2, PFE and PFW could be confirmed to have significant results. In regards to the measurement of mRNA expression of COL1A1, PFE displayed non-significant difference, PFW displayed significant difference. In regards to the generation of pro-collagen 1, the protein was generated for both PFE, PFW. In conclusion, PFE and PFW of Pini Folium extract can be applied to skin beauty practically and considered as the results to suggest the anti-aging possibility in aging skin in regards to mRNA expression of COL1A1 against NHDF and generation of pro-collagen 1.
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