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(초록) 용균성박테리오파지와그산생물이치료제제또는항생제의대체제로의사용이새롭게대두되면서살모넬라를포함한다약제내성을가진병원감염균의또다른치료제로의가능성이고려된다. Salmonella enterica serovar Enteritidis는주요한식품매개병원균중의하나이다. 이균은식품의표면에부착하여균막을형성할수있는능력을가지고있어서잠재적인감염의원인이될수있다. 박테리오파지는살모넬라와캠필로박터등의세균의집락화를감소시키는데유용하다. 본연구에서는Salmonella Enteritidis에특이적인용균성파지 (SE2 파지)를분리하여특성을규명하였다. SE2 파지의염...

(초록) 용균성박테리오파지와그산생물이치료제제또는항생제의대체제로의사용이새롭게대두되면서살모넬라를포함한다약제내성을가진병원감염균의또다른치료제로의가능성이고려된다. Salmonella enterica serovar Enteritidis는주요한식품매개병원균중의하나이다. 이균은식품의표면에부착하여균막을형성할수있는능력을가지고있어서잠재적인감염의원인이될수있다. 박테리오파지는살모넬라와캠필로박터등의세균의집락화를감소시키는데유용하다. 본연구에서는Salmonella Enteritidis에특이적인용균성파지 (SE2 파지)를분리하여특성을규명하였다. SE2 파지의염색체염기서열은용원성유발유전자가결핍되어있어서강력한용균성을보여준다. 이 SE2 파지는항균제내성을가지는Salmonella Enteritidis 부유세균의 99%와균막을형성한세균의 98% 이상을사멸시킬수있다. SE2 파지는극도의 pH와온도에대해서매우안정성을보였다. 이러한결과는 SE2 파지의Salmonella serovar Enteritidis에의한식품오염을차단하고, 살모넬라감염증예방과치료를할수있는생물학적제제로서의활용가능성을시사한다.

Abstract ▼ AI-Helper

Use of lytic bacteriophages as a therapeutic or biocontrol alternative of antibiotics and antibacterial agents have been renewed of interest. They have been considered as a therapeutic possibility against the multi-drug resistant (MDR) bacterial pathogens including Salmonella serovars. Salmonella en...

Use of lytic bacteriophages as a therapeutic or biocontrol alternative of antibiotics and antibacterial agents have been renewed of interest. They have been considered as a therapeutic possibility against the multi-drug resistant (MDR) bacterial pathogens including Salmonella serovars. Salmonella enterica serovar Enteritidis had been remained one of the major food borne pathogens. This organism has ability to form biofilms on food contact surfaces which may be the potential source and cause of infections. Bacteriophages had been found useful to reduce Salmonella and Campylobacter colonization in vivo and in vitro. I isolated and characterized a Salmonella Enteritidis and Gallinarum specific lytic bacteriophage, phageSE2. The morphological and genomic features revealed that phageSE2 belong to family Siphoviridae. Genomic sequence annotations of phageSE2 showed a prominent lytic in nature due to lacking the lysogenic related genes. PhageSE2 was stable with maintaining its virulence for long term storage at ambient and low temperature. It could resist extreme pH and temperature for an hour of exposure. It could lyse more than 5 logs of planktonic cells and 98% of biofilmed cells. It can be a potential biotherapeutic agent to prevent infections and contamination of food.

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