Abstract ▼ AI-Helper

This study is carried out to investigate the production of γ-Aminobutyric acid(GABA) by Lactobacillus brevis CMF20 and to manufacture optimum medium of using rice bran extract. When the Lactobacillus brevis CMF20 isolated from Kimchi was cultured in MRS broth, it produced GABA to the concentration o...

This study is carried out to investigate the production of γ-Aminobutyric acid(GABA) by Lactobacillus brevis CMF20 and to manufacture optimum medium of using rice bran extract. When the Lactobacillus brevis CMF20 isolated from Kimchi was cultured in MRS broth, it produced GABA to the concentration of 276.42㎍/㎖ at optimum conditions of 25℃ and pH 6.5. In order to determine the optimum conditions for GABA production, Lactobacillus brevis CMF20 was cultivated in MRS broth and rice bran extract containing 0.2, 0.4, 0.6, 0.8, 1.0% (w/v) monosodium glutamate(MSG), respectively. In the medium containing 0.8% MSG, Lactobacillus brevis CMF20 showed the highest GABA production up to the concentration of 1011.86㎍/㎖ in MRS broth and 447.40㎍/㎖ in rice bran extract. The production level of GABA was enhanced by adding MSG and 2% of sucrose and soytone, respectively in rice bran extract compared to the control. These data suggest that rice bran extract can be a economic commercial medium for GABA production as a substitute for MRS broth.