콩 육종포장에서 바이러스병 발생실태와 콩에서 Bean common mosaic virus의 최초 발생보고 Incidence of virus diseases on soybean breeding fields and first report of Bean common mosaic virus on soybean in Korea원문보기
콩에 발생하는 바이러스의 종류, 감염율 등을 조사하기 위해 2012년 7월 중순부터 8월 중순까지 전국 8개도 19개 시∙군 원종장, 원원종장, 기본식물 포장, 기능성작물부 대구시험지를 비롯한 콩 육종포장에서 바이러스 병징으로 생각되는 시료들을 채집하였다. 수집된 시료들은 14종 바이러스 AMV(Alfalfa mosaic virus), BBWV2(Broad bean wilt virus 2), BCMV(Bean common ...
콩에 발생하는 바이러스의 종류, 감염율 등을 조사하기 위해 2012년 7월 중순부터 8월 중순까지 전국 8개도 19개 시∙군 원종장, 원원종장, 기본식물 포장, 기능성작물부 대구시험지를 비롯한 콩 육종포장에서 바이러스 병징으로 생각되는 시료들을 채집하였다. 수집된 시료들은 14종 바이러스 AMV(Alfalfa mosaic virus), BBWV2(Broad bean wilt virus 2), BCMV(Bean common mosaic virus), BYMV(Bean yellow mosaic virus), CCMV(Cowpea chlorotic mottle virus), CMV(Cucumber mosaic virus), CPMV(Cowpea mosaic virus), PEMV(Peanut mottle virus), PSV(Peanut stunt virus), SbDV(Soybean dwarf virus), SMV (Soybean mosaic virus), SYMMV(Soybean yellow mottle mosaic virus), SYCMV(Soybean yellow common mosaic virus), TSV(Tobacco streak virus)에 대한 RT-PCR으로 검출하였다. 검출결과 SMV, PSV, SYCMV, SYMMV와 국내 미 보고종인 BCMV가 검출되었다. AMV, CMV, SbDV, CPMV, PEMV, CCMV, TSV, BBWV2 그리고 BYMV는 검출되지 않았다. 채집된 시료의 94.2%가 바이러스에 감염된 것으로 확인되고 5.8%의 시료에서 바이러스가 검출되지 않았다, 검출된 바이러스종들은 SMV 73.1%, PSV 6.5%, SYMMV 4.6%, SYCMV 1.9%로 확인 되었으며 단 한점의 시료에서만 BCMV가 검출되었다. 6.2% 시료에서 SMV와 PSV가 동시 검출되었으며, 0.8% 시료에서 SYMMV와 SYCMV가 동시 검출되었다. 국내 미보고 종인 BCMV의 전 염기서열 결정과 생물 검정을 실시하였다.
콩에 발생하는 바이러스의 종류, 감염율 등을 조사하기 위해 2012년 7월 중순부터 8월 중순까지 전국 8개도 19개 시∙군 원종장, 원원종장, 기본식물 포장, 기능성작물부 대구시험지를 비롯한 콩 육종포장에서 바이러스 병징으로 생각되는 시료들을 채집하였다. 수집된 시료들은 14종 바이러스 AMV(Alfalfa mosaic virus), BBWV2(Broad bean wilt virus 2), BCMV(Bean common mosaic virus), BYMV(Bean yellow mosaic virus), CCMV(Cowpea chlorotic mottle virus), CMV(Cucumber mosaic virus), CPMV(Cowpea mosaic virus), PEMV(Peanut mottle virus), PSV(Peanut stunt virus), SbDV(Soybean dwarf virus), SMV (Soybean mosaic virus), SYMMV(Soybean yellow mottle mosaic virus), SYCMV(Soybean yellow common mosaic virus), TSV(Tobacco streak virus)에 대한 RT-PCR으로 검출하였다. 검출결과 SMV, PSV, SYCMV, SYMMV와 국내 미 보고종인 BCMV가 검출되었다. AMV, CMV, SbDV, CPMV, PEMV, CCMV, TSV, BBWV2 그리고 BYMV는 검출되지 않았다. 채집된 시료의 94.2%가 바이러스에 감염된 것으로 확인되고 5.8%의 시료에서 바이러스가 검출되지 않았다, 검출된 바이러스종들은 SMV 73.1%, PSV 6.5%, SYMMV 4.6%, SYCMV 1.9%로 확인 되었으며 단 한점의 시료에서만 BCMV가 검출되었다. 6.2% 시료에서 SMV와 PSV가 동시 검출되었으며, 0.8% 시료에서 SYMMV와 SYCMV가 동시 검출되었다. 국내 미보고 종인 BCMV의 전 염기서열 결정과 생물 검정을 실시하였다.
To study incidence of virus diseases on soybean breeding fields in Korea, the 260 samples with viral symptoms were collected at 19 regions of 8 provinces in 2012. The RT-PCR assay was conducted to detect fourteen different viruses such as SMV (Soybean mosaic virus), SYMMV (Soybean yellow mottle mosa...
To study incidence of virus diseases on soybean breeding fields in Korea, the 260 samples with viral symptoms were collected at 19 regions of 8 provinces in 2012. The RT-PCR assay was conducted to detect fourteen different viruses such as SMV (Soybean mosaic virus), SYMMV (Soybean yellow mottle mosaic virus), SYCMV (Soybean yellow common mosaic virus), SbDV (Soybean dwarf virus), PSV (Peanut stunt virus), AMV (Alfalfa mosaic virus), CPMV (Cowpea mosaic virus), CMV (Cucumber mosaic virus), BCMV (Bean common mosaic virus), PEMV (Peanut mottle virus), CCMV (Cowpea chlorotic mottle virus), TSV (Tobacco streak virus), BBWV2 (Broad bean wilt virus 2) and BYMV (Bean yellow mosaic virus). Only five viruses such as SMV, PSV, SYMMV, and BCMV were shown to infect soybean. The infection rates of SMV, PSV, SYMMV, SYCMV and BCMV were 73.1, 6.5, 4.6, 1.9 and 0.4%, respectively. And three samples were co-infected by SYMMV and SYCMV but their symptoms were not different with other sample infected by SYCMV or SYMMV. This study could be the first report of the occurrence of the BCMV on soybean in Korea. To further characterize the virus, the complete nucleotide sequence was determined. On the basis of the phylogenetic analysis with complete genome.
To study incidence of virus diseases on soybean breeding fields in Korea, the 260 samples with viral symptoms were collected at 19 regions of 8 provinces in 2012. The RT-PCR assay was conducted to detect fourteen different viruses such as SMV (Soybean mosaic virus), SYMMV (Soybean yellow mottle mosaic virus), SYCMV (Soybean yellow common mosaic virus), SbDV (Soybean dwarf virus), PSV (Peanut stunt virus), AMV (Alfalfa mosaic virus), CPMV (Cowpea mosaic virus), CMV (Cucumber mosaic virus), BCMV (Bean common mosaic virus), PEMV (Peanut mottle virus), CCMV (Cowpea chlorotic mottle virus), TSV (Tobacco streak virus), BBWV2 (Broad bean wilt virus 2) and BYMV (Bean yellow mosaic virus). Only five viruses such as SMV, PSV, SYMMV, and BCMV were shown to infect soybean. The infection rates of SMV, PSV, SYMMV, SYCMV and BCMV were 73.1, 6.5, 4.6, 1.9 and 0.4%, respectively. And three samples were co-infected by SYMMV and SYCMV but their symptoms were not different with other sample infected by SYCMV or SYMMV. This study could be the first report of the occurrence of the BCMV on soybean in Korea. To further characterize the virus, the complete nucleotide sequence was determined. On the basis of the phylogenetic analysis with complete genome.
주제어
#virus diseases BCMV RACE PCR Bean common mosaic virus
학위논문 정보
저자
임승택
학위수여기관
경북대학교 대학원
학위구분
국내석사
학과
응용생명과학부 농생물학 전공
지도교수
이기운
발행연도
2013
총페이지
66 p.
키워드
virus diseases BCMV RACE PCR Bean common mosaic virus
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