Purpose: The purpose of this study was to investigate the effect of mixed mineral oil (MO) and hyaluronic acid (HA) eye drops on the tear film and ocular surface in a mouse model of experimental dry eye (EDE).
Methods: Eye drops consisting of mixed 0.1% HA and 0.1%, 0.5%, or 5.0% MO, or 0.1% HA were...
Purpose: The purpose of this study was to investigate the effect of mixed mineral oil (MO) and hyaluronic acid (HA) eye drops on the tear film and ocular surface in a mouse model of experimental dry eye (EDE).
Methods: Eye drops consisting of mixed 0.1% HA and 0.1%, 0.5%, or 5.0% MO, or 0.1% HA were applied to desiccating stress-induced murine dry eye. Tear volume, corneal irregularity score, tear film break-up time (TBUT), and corneal fluorescein staining scores were measured at 5 and 10 days after treatment. Levels of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, IL-17, interferon gamma-induced protein 10 (IP-10), and monokine induced by gamma-interferon (MIG) were measured in the conjunctiva using a multiplex immunobead assay at 10 days. Periodic acid-Schiff (PAS) staining was also performed.
Results: There was no significant difference in the tear volume between groups. The corneal irregularity score was lower in the 0.5% MO-treated group compared with the EDE group and HA-treated group. The 0.5% and 5.0% MO-treated groups showed a significant improvement in TBUT compared with the EDE group. Mice treated with 0.1% and 0.5% MO showed a significant improvement in corneal fluorescein staining score compared with the EDE and HA-treated groups. A significant decrease in the levels of TNF-α, IL-1β, IL-6, IL-17, IP-10, and MIG was observed in the HA- and 0.5% MO-treated groups compared with the EDE group. However, there were no significant differences in the cytokine and chemokine levels between the HA and MO groups. The conjunctival goblet cell count was higher in the 0.5% MO-treated group compared with the EDE group and HA-treated group.
Conclusions: The application of 0.5% MO and 0.1% HA mixture eye drops could lead to a better improvement in corneal irregularity, corneal fluorescein staining scores, and conjunctival goblet cell count compared with 0.1% HA eye drops in the treatment of experimental dry eye. These results suggest that the MO and HA mixture is more effective than the 0.1% HA eye drops for treating dry eye associated with ocular surface damage.
Purpose: The purpose of this study was to investigate the effect of mixed mineral oil (MO) and hyaluronic acid (HA) eye drops on the tear film and ocular surface in a mouse model of experimental dry eye (EDE).
Methods: Eye drops consisting of mixed 0.1% HA and 0.1%, 0.5%, or 5.0% MO, or 0.1% HA were applied to desiccating stress-induced murine dry eye. Tear volume, corneal irregularity score, tear film break-up time (TBUT), and corneal fluorescein staining scores were measured at 5 and 10 days after treatment. Levels of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, IL-17, interferon gamma-induced protein 10 (IP-10), and monokine induced by gamma-interferon (MIG) were measured in the conjunctiva using a multiplex immunobead assay at 10 days. Periodic acid-Schiff (PAS) staining was also performed.
Results: There was no significant difference in the tear volume between groups. The corneal irregularity score was lower in the 0.5% MO-treated group compared with the EDE group and HA-treated group. The 0.5% and 5.0% MO-treated groups showed a significant improvement in TBUT compared with the EDE group. Mice treated with 0.1% and 0.5% MO showed a significant improvement in corneal fluorescein staining score compared with the EDE and HA-treated groups. A significant decrease in the levels of TNF-α, IL-1β, IL-6, IL-17, IP-10, and MIG was observed in the HA- and 0.5% MO-treated groups compared with the EDE group. However, there were no significant differences in the cytokine and chemokine levels between the HA and MO groups. The conjunctival goblet cell count was higher in the 0.5% MO-treated group compared with the EDE group and HA-treated group.
Conclusions: The application of 0.5% MO and 0.1% HA mixture eye drops could lead to a better improvement in corneal irregularity, corneal fluorescein staining scores, and conjunctival goblet cell count compared with 0.1% HA eye drops in the treatment of experimental dry eye. These results suggest that the MO and HA mixture is more effective than the 0.1% HA eye drops for treating dry eye associated with ocular surface damage.
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