Abstract ▼ AI-Helper

Forensic RNA research using microRNA (miRNA) is just implicated in body fluid identification, but to understand of biological process in post-mortem pathology has not been proposed. Therefore, this study has performed animal experiments to compare miRNA expression pattern between fresh and salt wate...

Forensic RNA research using microRNA (miRNA) is just implicated in body fluid identification, but to understand of biological process in post-mortem pathology has not been proposed. Therefore, this study has performed animal experiments to compare miRNA expression pattern between fresh and salt water drowning model, and miRNA expression were analyzed in the brain through a forward bioinformatics screening approach. This study identified 8 new miRNAs which are increased in fresh water and decreased in salt water. Among them, microRNA-706(miR-706) as a potent biomarker for the drowning pattern identification to target HCN1 gene. miR-706 was higher expression of detected in fresh water drowning group than control and salt water drowning group (p<0.05, and p<0.01, respectively). HCN1 mRNA expression, suggested candidate target for miR-706, was decreased in fresh water group (p<0.01), and showed statistical difference between fresh and salt water drowning (p<0.01). And miR-706 was specifically expressed in hippocampal neuron by in situ hybridization. These results suggested that specific miRNA like miR-706 could be used as forensic biomarker for differentiation between fresh and salt water drowning.

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