인삼으로부터 진세노사이드 성분을 분리 동정하고 함량을 분석 하기 위하여 에탄올로 열수 추출하여 극성별로 분획하여 open silica gel 컬럼과 재컬럼 및 semiprep-HPLC 장비를 이용해 화합물을 분리 하였다. 이 화합물들을 핵자기공명분석법과 질량분석법을 이용해 ...
인삼으로부터 진세노사이드 성분을 분리 동정하고 함량을 분석 하기 위하여 에탄올로 열수 추출하여 극성별로 분획하여 open silica gel 컬럼과 재컬럼 및 semiprep-HPLC 장비를 이용해 화합물을 분리 하였다. 이 화합물들을 핵자기공명분석법과 질량분석법을 이용해 β-sitosterol (stigmast-5-en-3-ol) (1), daucosterol (2), adenosine (3), gypenoside V (4), ginsenoside Rb1 (5), ginsenoside Rb2 (6), ginsenoside Rb3 (7), ginsenoside Rc (8), ginsenoside Rd (9), ginsenoside Rs11 (10), 20(S)-notoginsenoside R2 (11), notoginsenosiede Rt (12), 20(S)-O-glucoginsenoside Rf (13), pseudoginsenoside Rt3 (14), ginsenoside Re (15), ginsenoside Re5 (16), ginsenoside Rf (17), ginsenoside Rg1 (18), ginsenoside Rg2 (19), ginsenoside Rh1 (20), ginsenoside Rg18 (21), 6-acetyl ginsenoside Rg3 (22), 6-O-[α-L-Rhamnopyranoside(1→2)-β-D-glucopyranoside]-20-O-β-D-glucopyranoside-3β,12β, 20(S)-dihydroxy-dammar-25-en-24-one (23), majotoside F6 (24), vinaginsenoside R15 (25), ginsenoside Re7 (26), ginsenoside Ro (27), 및 calenduloside B (28)로 동정하였다. 이 중 gypenoside V (4), notoginsenosiede-Rt (12), pseudoginsenoside Rt3 (14), 6-O-[α-L-rhamnopyranoside (1→2)-β-D-glucopyranoside]-20-O-β-D-gluco-pyranoside-3β,12β,20(S)-dihydroxy-dammar-25-en-24-one (23), majotoside F6 (24), vinaginsenoside R15 (25), 및calenduloside B (28)는 인삼에서 처음 분리 동정된 화합물이었으며, ginsenoside Rs11 (10), ginsenoside Rg18 (21), 6-acetyl ginsenoside Rg3 (22), 및 ginsenoside Re7 (26)는 자연계에서 처음 분리한 화합물이었다. 총 4종의 신규 진세노사이드 ginsenoside Rs11 (10), ginsenoside Rg18 (21), 6-acetyl ginsenoside Rg3 (22), 및 ginsenoside Re7 (26) 를 비교 분석했을 때, 금산 0.304 진안 0.199, 그리고 영주 인삼에서 0.192 mg/g으로 나타났으며 금산인삼에서 신규 진세노사이드에 대한 함량이 가장 많이 나타났다. 이와 같은 결과를 봤을 때 금산에서 재배된 인삼이 새로운 자원으로서의 가능성이 있는 것으로 생각된다. 또한 한국, 중국, 미국에서 재배되는 인삼에 대해서 신규 진세노사이드 함량을 비교 분석 하였다. 총 4종의 총 신규 진세노사이드에 함량에 대하여 한국 0.349, 중국 0.549, 그리고 미국 0.859 mg/g으로 나타났으며, 미국인삼에서 신규 진세노사이드에 대한 함량이 가장 많이 나타났다. 총 4종의 신규 진세노사이드에 대하여 홍삼, 직삼, 인삼의 연근별 비교분석을 하였다. 홍삼 4년근 0.500 mg/g, 5년근 0.377 mg/g, 6년근 0.410 mg/g, 직삼 4년근 0.162 mg/g, 직삼 5년근 0.220 mg/g, 직삼 6년근 0.170 mg/g, 인삼 4년근 0.379 mg/g, 5년근 0.349 mg/g, 인삼 6년근 0.33491 mg/g 과 같은 결과가 나타났다. 이와 같은 결과를 봤을때 재배 지역과 재배연도 따른 선택을 통해 선택적인 재배가 이루어 질수 있을 것이며, 이러한 결과들은 진세노사이드 화합물을 이용하는 기능성 식품, 제약 및 기능성 화장품 등의 분야에 정보로 사용 될 수 있을 것으로 생각된다.
인삼으로부터 진세노사이드 성분을 분리 동정하고 함량을 분석 하기 위하여 에탄올로 열수 추출하여 극성별로 분획하여 open silica gel 컬럼과 재컬럼 및 semiprep-HPLC 장비를 이용해 화합물을 분리 하였다. 이 화합물들을 핵자기공명분석법과 질량분석법을 이용해 β-sitosterol (stigmast-5-en-3-ol) (1), daucosterol (2), adenosine (3), gypenoside V (4), ginsenoside Rb1 (5), ginsenoside Rb2 (6), ginsenoside Rb3 (7), ginsenoside Rc (8), ginsenoside Rd (9), ginsenoside Rs11 (10), 20(S)-notoginsenoside R2 (11), notoginsenosiede Rt (12), 20(S)-O-glucoginsenoside Rf (13), pseudoginsenoside Rt3 (14), ginsenoside Re (15), ginsenoside Re5 (16), ginsenoside Rf (17), ginsenoside Rg1 (18), ginsenoside Rg2 (19), ginsenoside Rh1 (20), ginsenoside Rg18 (21), 6-acetyl ginsenoside Rg3 (22), 6-O-[α-L-Rhamnopyranoside(1→2)-β-D-glucopyranoside]-20-O-β-D-glucopyranoside-3β,12β, 20(S)-dihydroxy-dammar-25-en-24-one (23), majotoside F6 (24), vinaginsenoside R15 (25), ginsenoside Re7 (26), ginsenoside Ro (27), 및 calenduloside B (28)로 동정하였다. 이 중 gypenoside V (4), notoginsenosiede-Rt (12), pseudoginsenoside Rt3 (14), 6-O-[α-L-rhamnopyranoside (1→2)-β-D-glucopyranoside]-20-O-β-D-gluco-pyranoside-3β,12β,20(S)-dihydroxy-dammar-25-en-24-one (23), majotoside F6 (24), vinaginsenoside R15 (25), 및calenduloside B (28)는 인삼에서 처음 분리 동정된 화합물이었으며, ginsenoside Rs11 (10), ginsenoside Rg18 (21), 6-acetyl ginsenoside Rg3 (22), 및 ginsenoside Re7 (26)는 자연계에서 처음 분리한 화합물이었다. 총 4종의 신규 진세노사이드 ginsenoside Rs11 (10), ginsenoside Rg18 (21), 6-acetyl ginsenoside Rg3 (22), 및 ginsenoside Re7 (26) 를 비교 분석했을 때, 금산 0.304 진안 0.199, 그리고 영주 인삼에서 0.192 mg/g으로 나타났으며 금산인삼에서 신규 진세노사이드에 대한 함량이 가장 많이 나타났다. 이와 같은 결과를 봤을 때 금산에서 재배된 인삼이 새로운 자원으로서의 가능성이 있는 것으로 생각된다. 또한 한국, 중국, 미국에서 재배되는 인삼에 대해서 신규 진세노사이드 함량을 비교 분석 하였다. 총 4종의 총 신규 진세노사이드에 함량에 대하여 한국 0.349, 중국 0.549, 그리고 미국 0.859 mg/g으로 나타났으며, 미국인삼에서 신규 진세노사이드에 대한 함량이 가장 많이 나타났다. 총 4종의 신규 진세노사이드에 대하여 홍삼, 직삼, 인삼의 연근별 비교분석을 하였다. 홍삼 4년근 0.500 mg/g, 5년근 0.377 mg/g, 6년근 0.410 mg/g, 직삼 4년근 0.162 mg/g, 직삼 5년근 0.220 mg/g, 직삼 6년근 0.170 mg/g, 인삼 4년근 0.379 mg/g, 5년근 0.349 mg/g, 인삼 6년근 0.33491 mg/g 과 같은 결과가 나타났다. 이와 같은 결과를 봤을때 재배 지역과 재배연도 따른 선택을 통해 선택적인 재배가 이루어 질수 있을 것이며, 이러한 결과들은 진세노사이드 화합물을 이용하는 기능성 식품, 제약 및 기능성 화장품 등의 분야에 정보로 사용 될 수 있을 것으로 생각된다.
Phytochemical constituents were isolated from the Panax ginseng roots by repeated chromatography, HSCCC, and semiprep-HPLC. Their structures were elucidated as β-sitosterol (1), daucosterol (2), adenosine (3), gypenoside V (4), ginsenoside Rb1 (5), ginsenoside Rb2 (6), ginsenoside Rb3 (7), ginsenosi...
Phytochemical constituents were isolated from the Panax ginseng roots by repeated chromatography, HSCCC, and semiprep-HPLC. Their structures were elucidated as β-sitosterol (1), daucosterol (2), adenosine (3), gypenoside V (4), ginsenoside Rb1 (5), ginsenoside Rb2 (6), ginsenoside Rb3 (7), ginsenoside Rc (8), ginsenoside Rd (9), ginsenoside Rs11 (10), 20(S)-notoginsenoside R2 (11), notoginsenosiede Rt (12), 20(S)-O-glucoginsenoside Rf (13), pseudoginsenoside Rt3 (14), ginsenoside Re (15), ginsenoside Re5 (16), ginsenoside Rf (17), ginsenoside Rg1 (18), ginsenoside Rg2 (19), ginsenoside Rh1 (20), ginsenoside Rg18 (21), 6-acetyl ginsenoside Rg3 (22), 6-O-[α-L-rhamnopyranoside (1→2)-β-D-glucopyranosside]-20-O-β-D-gluco-pyranoside-3β,12β,20(S)-dihydroxydammar-25-en-24-one (23), majoroside F6 (24), vinaginsenoside R15 (25), ginsenoside Re7 (26), calenduloside-B (27), and ginsenoside Ro (28). Among them gypenoside-V (4), notoginsenosiede Rt (12), pseudoginsenoside-Rt3 (14), 6-O-[α-L-rhamnopyranoside (1→2)-β-D-glucopyranoside]-20-O-β-D-gluco-pyranoside-3β,12β,20(S)-dihydroxydammar-25-en-24-one (23), majoroside F6 (24), vinaginsenoside R15 (25), and calenduloside B (27) were isolated for the first time from P. ginseng roots. Also, ginsenoside Rs11 (10), ginsenoside Rg18 (21), 6-acetyl ginsenoside Rg3 (22), and ginsenoside Re7 (26) were isolated for the first time from nature. The new ginsenosides in ginseng species (different areas; Geumsan, Yeongju and Jinan, countries; P. ginseng (Korea), P. notoginseng (China), P. quinquefolium (America), production process; straight-, red- and white-ginseng, cultivation years; 4-year-old, 5-year-old and 6-year-old were determined by HPLC/UV. The content of new ginsenosides in ginseng species was investigated in different production process and cultivative years; the contents of compounds 10, 21, 22, and 26 were highest in red-4 yr (0.018 mg/g), white-5 yr (0.024 mg/g), red-6 yr (0.022 mg/g), and white-6 yr (0.106 mg/g), respectively. And, the content of new ginsenosides in ginseng species cultivated in different area in Korea, the contents of compound 10, 21, 22, and 26 were highest in Jinan (0.007 mg/g), Jinan (0.020 mg/g), Jinan (0.008 mg/g), and Geumsan (0.161 mg/g), respectively. Also, the content of new ginsenosides in ginseng species cultivated in different countries; the contents of compounds 10, 21, 22, and 26 were highest in P. quinquefolium (0.023 mg/g), P. ginseng (0.025 mg/g), P. quinquefolium (0.031 mg/g), and P. quinquefolium (0.097 mg/g), respectively. In the total content of compounds 10, 21, 22, and 26 in ginseng species cultivated in different area in Korea that of Geumsan was higher than those of Yeongju and Jinan. And the total content of compounds 10, 21, 22, and 26 in ginseng species in cultivation in different countries, P. quinquefolium (1.773 mg/g) was higher than those of P. notoginseng and P. ginseng. The total content of compounds 10, 21, 22, and 26 in ginseng species in different production process and cultivation years that of red-4 yr was higher than those cultivated for longer periods (red-5 yr and red-6 yr). And white ginseng also showed the same tendency. These results will be useful information in the application of these ginseng in the nutraceutical, pharmaceutical, and cosmeceutical industries.
Phytochemical constituents were isolated from the Panax ginseng roots by repeated chromatography, HSCCC, and semiprep-HPLC. Their structures were elucidated as β-sitosterol (1), daucosterol (2), adenosine (3), gypenoside V (4), ginsenoside Rb1 (5), ginsenoside Rb2 (6), ginsenoside Rb3 (7), ginsenoside Rc (8), ginsenoside Rd (9), ginsenoside Rs11 (10), 20(S)-notoginsenoside R2 (11), notoginsenosiede Rt (12), 20(S)-O-glucoginsenoside Rf (13), pseudoginsenoside Rt3 (14), ginsenoside Re (15), ginsenoside Re5 (16), ginsenoside Rf (17), ginsenoside Rg1 (18), ginsenoside Rg2 (19), ginsenoside Rh1 (20), ginsenoside Rg18 (21), 6-acetyl ginsenoside Rg3 (22), 6-O-[α-L-rhamnopyranoside (1→2)-β-D-glucopyranosside]-20-O-β-D-gluco-pyranoside-3β,12β,20(S)-dihydroxydammar-25-en-24-one (23), majoroside F6 (24), vinaginsenoside R15 (25), ginsenoside Re7 (26), calenduloside-B (27), and ginsenoside Ro (28). Among them gypenoside-V (4), notoginsenosiede Rt (12), pseudoginsenoside-Rt3 (14), 6-O-[α-L-rhamnopyranoside (1→2)-β-D-glucopyranoside]-20-O-β-D-gluco-pyranoside-3β,12β,20(S)-dihydroxydammar-25-en-24-one (23), majoroside F6 (24), vinaginsenoside R15 (25), and calenduloside B (27) were isolated for the first time from P. ginseng roots. Also, ginsenoside Rs11 (10), ginsenoside Rg18 (21), 6-acetyl ginsenoside Rg3 (22), and ginsenoside Re7 (26) were isolated for the first time from nature. The new ginsenosides in ginseng species (different areas; Geumsan, Yeongju and Jinan, countries; P. ginseng (Korea), P. notoginseng (China), P. quinquefolium (America), production process; straight-, red- and white-ginseng, cultivation years; 4-year-old, 5-year-old and 6-year-old were determined by HPLC/UV. The content of new ginsenosides in ginseng species was investigated in different production process and cultivative years; the contents of compounds 10, 21, 22, and 26 were highest in red-4 yr (0.018 mg/g), white-5 yr (0.024 mg/g), red-6 yr (0.022 mg/g), and white-6 yr (0.106 mg/g), respectively. And, the content of new ginsenosides in ginseng species cultivated in different area in Korea, the contents of compound 10, 21, 22, and 26 were highest in Jinan (0.007 mg/g), Jinan (0.020 mg/g), Jinan (0.008 mg/g), and Geumsan (0.161 mg/g), respectively. Also, the content of new ginsenosides in ginseng species cultivated in different countries; the contents of compounds 10, 21, 22, and 26 were highest in P. quinquefolium (0.023 mg/g), P. ginseng (0.025 mg/g), P. quinquefolium (0.031 mg/g), and P. quinquefolium (0.097 mg/g), respectively. In the total content of compounds 10, 21, 22, and 26 in ginseng species cultivated in different area in Korea that of Geumsan was higher than those of Yeongju and Jinan. And the total content of compounds 10, 21, 22, and 26 in ginseng species in cultivation in different countries, P. quinquefolium (1.773 mg/g) was higher than those of P. notoginseng and P. ginseng. The total content of compounds 10, 21, 22, and 26 in ginseng species in different production process and cultivation years that of red-4 yr was higher than those cultivated for longer periods (red-5 yr and red-6 yr). And white ginseng also showed the same tendency. These results will be useful information in the application of these ginseng in the nutraceutical, pharmaceutical, and cosmeceutical industries.
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