Abstract ▼ AI-Helper

The study was indicated that dietary natural phytoncide feed additive alter either immune-related serum substances or serum metabolites in Hanwoo bulls. Cypress (CYP) and mugwort (MUG) was treated with 0.5% of concentrate diet for 90 days. A total of 15 bulls with initial BW of control 196.00±5.33kg...

The study was indicated that dietary natural phytoncide feed additive alter either immune-related serum substances or serum metabolites in Hanwoo bulls. Cypress (CYP) and mugwort (MUG) was treated with 0.5% of concentrate diet for 90 days. A total of 15 bulls with initial BW of control 196.00±5.33kg, treatment 1 (CYP) 196.00±3.91kg, treatment 2 (MUG) 196.00±3.31kg were used 90 days on feed experiment. We are analyzed for changes of immunological substances in the serum by processing LPS (Lipopolysaccharide) challenge. To reduce stress of individual animal the jugular vein catheter was installed to each cattle and domesticated for 24 hours. Blood was performed a total of 13 times sampling every 30 minutes after the third blood was injection to the LPS (1㎍/㎏ of BW). Serum Albumin (ALB), Glucose (GLU), Total protein (TP), Triglyceride (TG), Phosphorus (IP), Non-esterified fatty acid (NEFA), cortisol, Interleukin-6 (IL-6), Interleukin-1β (IL-1ß), Tumor Necrosis Factor ⍺ (TNF⍺) were analyzed with standard procedures. After LPS injection, serum levels of ALB and NEFA tended to increase (P>0.05) and serum GLU, TG, IP and TP were not effected by the treatment of phytoncide. Proinflammatory cytokine such as IL-1β, IL-6 and TNFα increased during time courses. However, the level of serum TNFα tends to be decreased the treatment of phytoncide (p0.05). In conclusion, dietary supplementation of natural phytoncide not only increased serum metabolites, but also decreased proinflammatory cytokines in Hanwoo bulls under LPS challenge.

Keyword