ABSTRACT Analysis of Skin Mechanism and an Application for Functional Cosmetic from Red Bean (Phaseolus angularis) Shell Extract. Soo Yeon Lee Department of Cosmetic Science, The graduate School Hoseo University, Asan, Korea (Supervised by professor Jin Young Lee)The extract of Phaseolus angularis s...
ABSTRACT Analysis of Skin Mechanism and an Application for Functional Cosmetic from Red Bean (Phaseolus angularis) Shell Extract. Soo Yeon Lee Department of Cosmetic Science, The graduate School Hoseo University, Asan, Korea (Supervised by professor Jin Young Lee)The extract of Phaseolus angularis shell was investigated for the activities of anti-wrinkle, whitening and anti-inflammatory effects to apply as a functional ingredient for cosmetic products. We isolated four compounds (catechin, rutin, iso-quercitrin, linoleic acid) of extract from Phaseolus angularis shell using INNO column. The electron donating ability of extract from Phaseolus angularis shell was above 67.2% at the concentration of 1,000 μg/ml. The tyrosinase inhibitory effect which is related to skin-whitening, was 76.4% at the concentration of 1,000 μg/ml. For anti-wrinkle effect, the elastase and collagenase inhibition activities of Phaseolus angularis shell were 75% and 64.7% at the concentration of 1,000 μg/ml respectively. Melanoma cell (B16F10), fibroblast cell (CCD-986sk) and macrophage cell (Raw264.7) by measuring the cell toxicity was perfomed to test the cell viability at a concentration which does not toxicity to cells of at least 90% of the extract from Phaseolus angularis shell. The result of measuring the apoptosis and necrosis of the three cell lines according to the extract from Phaseolus angularis shell with Annexin V, it was confirmed that the cells did not affect it for growth when compared with control group and cell population treated extract from Phaseolus angularis shell. In nitric oxide synthesis inhibition effect of extract from Phaseolus angularis shell was shown 67.4% at the concentration of 500 μg/ml. Also, the result of measuring the effect of phaseolus angularis shell extract on intracellular ROS level in LPS stimulated Raw264.7 macrophage cell. it was significantly decreased in treated extract from Phaseolus angularis shell group. The MITF, TRP-1, TRP-2, tyrosinase, MMP-1, MMP-2, MMP-3, iNOS and COX-2 protein expression inhibitory effect by western blot of extract from Phaseolus angularis shell was decreased dependent concentration. Also the MITF, TRP-1, TRP-2, tyrosinase, MMP-1, MMP-2, MMP-3, iNOS and COX-2 mRNA expression inhibitory effect by reverse transcription-PCR of extract from Phaseolus angularis shell was decreased dependent concentration too. We measured chemokines such as regulated on activation normal T-cell expression and screated (RANTES), macrophage-drived chemokine (MDC), interferon-inducible protein-10 (IP-10) for anti-wrinkle and anti-inflammatory effects of extract from Phaseolus angularis Shell in the HaCaT cell by responsed TNF-α+IFN-γ-induced inflammatory. Consequently it these chemokines were decreased more than 50 μg/ml concentration. We investigated anti-wrinkle and anti-pruritus effects of extract from Phaseolus angularis shell in hairless mice (HR-1) dorsal skin. In our experimenral condition, skin thickness of hairless mice was significantly decreased in treated extract from Phaseolus angularis shell group campared with control group. And also decreased cell transformation, hyperkeratinization, pigmentation and infiltration of cornification symptom and mast cells in around skin. IL-31 which is biomarker with pruritus by skin photoaging related quantitative mRNA expression rate by real time-PCR of extract from Phaseolus angularis shell was significantly decreased in treated extract from Phaseolus angularis shell group compared with control grop. All these findings suggested that extract from Phaseolus angularis shell has a great potential as a cosmeceutical ingredients with whitening, anti-wrinkle and anti-inflammatory effect.
ABSTRACT Analysis of Skin Mechanism and an Application for Functional Cosmetic from Red Bean (Phaseolus angularis) Shell Extract. Soo Yeon Lee Department of Cosmetic Science, The graduate School Hoseo University, Asan, Korea (Supervised by professor Jin Young Lee)The extract of Phaseolus angularis shell was investigated for the activities of anti-wrinkle, whitening and anti-inflammatory effects to apply as a functional ingredient for cosmetic products. We isolated four compounds (catechin, rutin, iso-quercitrin, linoleic acid) of extract from Phaseolus angularis shell using INNO column. The electron donating ability of extract from Phaseolus angularis shell was above 67.2% at the concentration of 1,000 μg/ml. The tyrosinase inhibitory effect which is related to skin-whitening, was 76.4% at the concentration of 1,000 μg/ml. For anti-wrinkle effect, the elastase and collagenase inhibition activities of Phaseolus angularis shell were 75% and 64.7% at the concentration of 1,000 μg/ml respectively. Melanoma cell (B16F10), fibroblast cell (CCD-986sk) and macrophage cell (Raw264.7) by measuring the cell toxicity was perfomed to test the cell viability at a concentration which does not toxicity to cells of at least 90% of the extract from Phaseolus angularis shell. The result of measuring the apoptosis and necrosis of the three cell lines according to the extract from Phaseolus angularis shell with Annexin V, it was confirmed that the cells did not affect it for growth when compared with control group and cell population treated extract from Phaseolus angularis shell. In nitric oxide synthesis inhibition effect of extract from Phaseolus angularis shell was shown 67.4% at the concentration of 500 μg/ml. Also, the result of measuring the effect of phaseolus angularis shell extract on intracellular ROS level in LPS stimulated Raw264.7 macrophage cell. it was significantly decreased in treated extract from Phaseolus angularis shell group. The MITF, TRP-1, TRP-2, tyrosinase, MMP-1, MMP-2, MMP-3, iNOS and COX-2 protein expression inhibitory effect by western blot of extract from Phaseolus angularis shell was decreased dependent concentration. Also the MITF, TRP-1, TRP-2, tyrosinase, MMP-1, MMP-2, MMP-3, iNOS and COX-2 mRNA expression inhibitory effect by reverse transcription-PCR of extract from Phaseolus angularis shell was decreased dependent concentration too. We measured chemokines such as regulated on activation normal T-cell expression and screated (RANTES), macrophage-drived chemokine (MDC), interferon-inducible protein-10 (IP-10) for anti-wrinkle and anti-inflammatory effects of extract from Phaseolus angularis Shell in the HaCaT cell by responsed TNF-α+IFN-γ-induced inflammatory. Consequently it these chemokines were decreased more than 50 μg/ml concentration. We investigated anti-wrinkle and anti-pruritus effects of extract from Phaseolus angularis shell in hairless mice (HR-1) dorsal skin. In our experimenral condition, skin thickness of hairless mice was significantly decreased in treated extract from Phaseolus angularis shell group campared with control group. And also decreased cell transformation, hyperkeratinization, pigmentation and infiltration of cornification symptom and mast cells in around skin. IL-31 which is biomarker with pruritus by skin photoaging related quantitative mRNA expression rate by real time-PCR of extract from Phaseolus angularis shell was significantly decreased in treated extract from Phaseolus angularis shell group compared with control grop. All these findings suggested that extract from Phaseolus angularis shell has a great potential as a cosmeceutical ingredients with whitening, anti-wrinkle and anti-inflammatory effect.
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