This study was focused on, investigate the phenolic compound compositions, cosmetic physiological activities, and natural hair dye of R. tinctorum root with hot water and 80% methanol extraction. The results were as follows; Total polyphenol content was found was 340.05 ± 0.52 mg% in hot water extra...
This study was focused on, investigate the phenolic compound compositions, cosmetic physiological activities, and natural hair dye of R. tinctorum root with hot water and 80% methanol extraction. The results were as follows; Total polyphenol content was found was 340.05 ± 0.52 mg% in hot water extract and 485.50 ± 2.39 mg% in 80% methanol extract. It indicated that 80% methanol extract was slightly higher than hot water extract. Similarly, total flavonoid content was found 119.36 ± 1.10 mg% in the hot water extract and 108.64 ± 1.31 mg% in methanol extract. This result indicated that total flavonoid content was higher in hot water extract than methanol extract. Three phenolic compounds namely, protocatechuic acid, chlorogenic acid and caffeic acid, and zero type of flavonoids was found in hot-water extraction where as, four different types of phenolic compounds namely, chlorogenic acid, caffeic acid, t-cinnamic acid and salycilic acid of phenolic acids and one type, quercetin of flavonoids in methanol extracts. The DPPH radical scavenging activity of R. tinctorum root with hot water extract and methanol extract was 61.90 ± 0.03% and 59.46 ± 1.35%, respectively. It was concluded that DPPH radical scavenging activity of R. tinctorum root was slightly higher in hot water extract. When hot water extract of R. tinctorum root was added to the human keratinocyte cell line HaCaT, then cells grew in 20 ㎍/ml, on the other hand, cytotoxicity was increased by about 22% as compare to the control group until 100 ㎍/ml. When hair dyeing with R. tinctorum root pigment dyed at 40℃ and 50℃ in no-mordanting and pre-mordanting respectively for 20 minutes was best conditions. In addition, the color varies depending on the level of mordants(KAl(SO4)2‧l2H2O). If no mordant was used, red colour was appeared while when a pre-mordanting was used, reddish brown was obtained. In post-mordanting, hair color was more dens color than no-mordanting. In terms of color fastness of dyed hair cleansing, no, pre and post-mordanting, and there was almost no change in L*, a*, b* and △E. Therefore, it is confirmed that the hair dyed with R. tinctorum root pigment has very superior color fastness to dyed hair cleansing and light handling as well, it was very stable. In conclusion, R. tinctorum root extracts are functional and effective. More over, they would be very useful as a natural antioxidants and natural hair dye cosmetic material. However, there should be further studies on natural pigments for the development of natural hair colors.
This study was focused on, investigate the phenolic compound compositions, cosmetic physiological activities, and natural hair dye of R. tinctorum root with hot water and 80% methanol extraction. The results were as follows; Total polyphenol content was found was 340.05 ± 0.52 mg% in hot water extract and 485.50 ± 2.39 mg% in 80% methanol extract. It indicated that 80% methanol extract was slightly higher than hot water extract. Similarly, total flavonoid content was found 119.36 ± 1.10 mg% in the hot water extract and 108.64 ± 1.31 mg% in methanol extract. This result indicated that total flavonoid content was higher in hot water extract than methanol extract. Three phenolic compounds namely, protocatechuic acid, chlorogenic acid and caffeic acid, and zero type of flavonoids was found in hot-water extraction where as, four different types of phenolic compounds namely, chlorogenic acid, caffeic acid, t-cinnamic acid and salycilic acid of phenolic acids and one type, quercetin of flavonoids in methanol extracts. The DPPH radical scavenging activity of R. tinctorum root with hot water extract and methanol extract was 61.90 ± 0.03% and 59.46 ± 1.35%, respectively. It was concluded that DPPH radical scavenging activity of R. tinctorum root was slightly higher in hot water extract. When hot water extract of R. tinctorum root was added to the human keratinocyte cell line HaCaT, then cells grew in 20 ㎍/ml, on the other hand, cytotoxicity was increased by about 22% as compare to the control group until 100 ㎍/ml. When hair dyeing with R. tinctorum root pigment dyed at 40℃ and 50℃ in no-mordanting and pre-mordanting respectively for 20 minutes was best conditions. In addition, the color varies depending on the level of mordants(KAl(SO4)2‧l2H2O). If no mordant was used, red colour was appeared while when a pre-mordanting was used, reddish brown was obtained. In post-mordanting, hair color was more dens color than no-mordanting. In terms of color fastness of dyed hair cleansing, no, pre and post-mordanting, and there was almost no change in L*, a*, b* and △E. Therefore, it is confirmed that the hair dyed with R. tinctorum root pigment has very superior color fastness to dyed hair cleansing and light handling as well, it was very stable. In conclusion, R. tinctorum root extracts are functional and effective. More over, they would be very useful as a natural antioxidants and natural hair dye cosmetic material. However, there should be further studies on natural pigments for the development of natural hair colors.
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