In this study, we firstly investigated the distribution of L. pneumophila in water systems of public facilities in Busan. L. pneumophila was isolated from 254 samples (8.3%) among 3,055 samples analyzed in this study. The highest isolation rates according to facilities and sample type were 49.0% (24...
In this study, we firstly investigated the distribution of L. pneumophila in water systems of public facilities in Busan. L. pneumophila was isolated from 254 samples (8.3%) among 3,055 samples analyzed in this study. The highest isolation rates according to facilities and sample type were 49.0% (24/49) in ships and 10.4% (120/1,156) in cooling tower water, respectively, while the lowest isolation rates were 4.2% (4/94) in the fountains and 6.8% (48/701) in cold water, respectively. The serological distribution of L. pneumophila isolates was surveyed according to the time period, facilities, and the sample type. The serogroups isolated in 2007 were distributed to sg 1-7 except for sg 4, and serogroups of sg 8-15 were not found at all. In comparison, much more diverse serotypes with the predominant strain sg 1 (27.3%) were isolated during 2013-2014. Among the 254 isolates, L. pneumophila sg 1 predominated in facilities such as buildings (43.9%), public baths (36.6%), hospitals (37.7%), hotels (60.0%), and fountains (75.0%) but not in ships and factories. L. pneumophila sg 3 and sg 7 were prevalent in ships (45.8%) and in factories (40.0%), respectively. The predominated serogroup of isolates in hot and cooling tower water was L. pneumophila sg 1 (34.9% and 45.8%, respectively), while sg 3 (29.2%) in cold water. And then we investigated the distribution of three virulence loci (dot/icm, lvh, and rtxA) in isolates from Busan by PCR. A high frequency of environmental and clinical isolates were positive for all three loci (50% and 75%, respectively), whereas the percentages of environmental and clinical isolates harboring dot/icm and rtxA were 20.9% and 25%, respectively. These results indicate that there is a difference in the distribution of virulence genes between clinical and environment isolates, although the distribution patterns are similar. Among the 254 environmental isolates investigated, 23 isolates (9.1%) carried lvh and rtxA, and 29 isolates (11.4%) harbored dot/icm and lvh, whereas only 0.4–4.3% of isolates had one gene. In addition, 67.7% of isolates from serogroup 1, the main cause of legionellosis, possessed all three loci, whereas 39.8% of isolates from serogroups 2 to 15 had all three loci. The positive rates for isolates with two loci were 5.4–14% in serogroup 1 and 6.2–28.6% in serogroups 2 to 15, whereas those for one loci were 1.1–2.2% in serogroup 1 and 0–5.6% in serogroups 2 to 15. These results may be useful for epidemiological survey to pursuit the sources of outbreaks of legionellosis in Busan.
In this study, we firstly investigated the distribution of L. pneumophila in water systems of public facilities in Busan. L. pneumophila was isolated from 254 samples (8.3%) among 3,055 samples analyzed in this study. The highest isolation rates according to facilities and sample type were 49.0% (24/49) in ships and 10.4% (120/1,156) in cooling tower water, respectively, while the lowest isolation rates were 4.2% (4/94) in the fountains and 6.8% (48/701) in cold water, respectively. The serological distribution of L. pneumophila isolates was surveyed according to the time period, facilities, and the sample type. The serogroups isolated in 2007 were distributed to sg 1-7 except for sg 4, and serogroups of sg 8-15 were not found at all. In comparison, much more diverse serotypes with the predominant strain sg 1 (27.3%) were isolated during 2013-2014. Among the 254 isolates, L. pneumophila sg 1 predominated in facilities such as buildings (43.9%), public baths (36.6%), hospitals (37.7%), hotels (60.0%), and fountains (75.0%) but not in ships and factories. L. pneumophila sg 3 and sg 7 were prevalent in ships (45.8%) and in factories (40.0%), respectively. The predominated serogroup of isolates in hot and cooling tower water was L. pneumophila sg 1 (34.9% and 45.8%, respectively), while sg 3 (29.2%) in cold water. And then we investigated the distribution of three virulence loci (dot/icm, lvh, and rtxA) in isolates from Busan by PCR. A high frequency of environmental and clinical isolates were positive for all three loci (50% and 75%, respectively), whereas the percentages of environmental and clinical isolates harboring dot/icm and rtxA were 20.9% and 25%, respectively. These results indicate that there is a difference in the distribution of virulence genes between clinical and environment isolates, although the distribution patterns are similar. Among the 254 environmental isolates investigated, 23 isolates (9.1%) carried lvh and rtxA, and 29 isolates (11.4%) harbored dot/icm and lvh, whereas only 0.4–4.3% of isolates had one gene. In addition, 67.7% of isolates from serogroup 1, the main cause of legionellosis, possessed all three loci, whereas 39.8% of isolates from serogroups 2 to 15 had all three loci. The positive rates for isolates with two loci were 5.4–14% in serogroup 1 and 6.2–28.6% in serogroups 2 to 15, whereas those for one loci were 1.1–2.2% in serogroup 1 and 0–5.6% in serogroups 2 to 15. These results may be useful for epidemiological survey to pursuit the sources of outbreaks of legionellosis in Busan.
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#Legionella pneumophila 분리율 혈청형 레지오넬라증
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